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Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation

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Abstract
Adhesion to the intestinal epithelium could constitute an essential mechanism of Bacillus cereus pathogenesis. However, the enterocytes are protected by mucus, a secretion composed mainly of mucin glycoproteins. These may serve as nutrients and sites of adhesion for intestinal bacteria. In this study, the food poisoning bacterium B. cereus NVH 0500/00 was exposed in vitro to gastrointestinal hurdles prior to evaluation of its attachment to mucin microcosms and its ability to produce nonhemolytic enterotoxin (Nhe). The persistence of mucin-adherent B. cereus after simulated gut emptying was determined using a mucin adhesion assay. The stability of Nhe toward bile and pancreatin (intestinal components) in the presence of mucin agar was also investigated. B. cereus could grow and simultaneously adhere to mucin during in vitro ileal incubation, despite the adverse effect of prior exposure to a low pH or intestinal components. The final concentration of B. cereus in the simulated lumen at 8 h of incubation was 6.62 +/- 0.87 log CFU ml(-1). At that point, the percentage of adhesion was approximately 6%. No enterotoxin was detected in the ileum, due to either insufficient bacterial concentrations or Nhe degradation. Nevertheless, mucin appears to retain B. cereus and to supply it to the small intestine after simulated gut emptying. Additionally, mucin may play a role in the protection of enterotoxins from degradation by intestinal components.
Keywords
IN-VITRO SIMULATION, ACID TOLERANCE RESPONSE, VEGETATIVE CELLS, BIOFILM FORMATION, EPITHELIAL-CELLS, SMALL-INTESTINE, HEMOLYSIN BL, SPORES, SURVIVAL, STRAINS

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Chicago
Tsilia, Varvara, Frederiek-Maarten Kerckhof, Andreja Rajkovic, Marc Heyndrickx, and Tom Van de Wiele. 2016. “Bacillus Cereus NVH 0500/00 Can Adhere to Mucin but Cannot Produce Enterotoxins During Gastrointestinal Simulation.” Applied and Environmental Microbiology 82 (1): 289–296.
APA
Tsilia, V., Kerckhof, F.-M., Rajkovic, A., Heyndrickx, M., & Van de Wiele, T. (2016). Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(1), 289–296.
Vancouver
1.
Tsilia V, Kerckhof F-M, Rajkovic A, Heyndrickx M, Van de Wiele T. Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2016;82(1):289–96.
MLA
Tsilia, Varvara, Frederiek-Maarten Kerckhof, Andreja Rajkovic, et al. “Bacillus Cereus NVH 0500/00 Can Adhere to Mucin but Cannot Produce Enterotoxins During Gastrointestinal Simulation.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 82.1 (2016): 289–296. Print.
@article{7039769,
  abstract     = {Adhesion to the intestinal epithelium could constitute an essential mechanism of Bacillus cereus pathogenesis. However, the enterocytes are protected by mucus, a secretion composed mainly of mucin glycoproteins. These may serve as nutrients and sites of adhesion for intestinal bacteria. In this study, the food poisoning bacterium B. cereus NVH 0500/00 was exposed in vitro to gastrointestinal hurdles prior to evaluation of its attachment to mucin microcosms and its ability to produce nonhemolytic enterotoxin (Nhe). The persistence of mucin-adherent B. cereus after simulated gut emptying was determined using a mucin adhesion assay. The stability of Nhe toward bile and pancreatin (intestinal components) in the presence of mucin agar was also investigated. B. cereus could grow and simultaneously adhere to mucin during in vitro ileal incubation, despite the adverse effect of prior exposure to a low pH or intestinal components. The final concentration of B. cereus in the simulated lumen at 8 h of incubation was 6.62 +/- 0.87 log CFU ml(-1). At that point, the percentage of adhesion was approximately 6\%. No enterotoxin was detected in the ileum, due to either insufficient bacterial concentrations or Nhe degradation. Nevertheless, mucin appears to retain B. cereus and to supply it to the small intestine after simulated gut emptying. Additionally, mucin may play a role in the protection of enterotoxins from degradation by intestinal components.},
  author       = {Tsilia, Varvara and Kerckhof, Frederiek-Maarten and Rajkovic, Andreja and Heyndrickx, Marc and Van de Wiele, Tom},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {289--296},
  title        = {Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation},
  url          = {http://dx.doi.org/10.1128/AEM.02940-15},
  volume       = {82},
  year         = {2016},
}

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