Ghent University Academic Bibliography

Advanced

Diagnostic accuracy of urinary prostate protein glycosylation profiling in prostatitis diagnosis

Tijl Vermassen, Charles Van Praet UGent, Filip Poelaert UGent, Nicolaas Lumen UGent, Karel Decaestecker UGent, Piet Hoebeke UGent, Simon Van Belle UGent, Sylvie Rottey UGent and Joris Delanghe UGent (2015) BIOCHEMIA MEDICA. 25(3). p.439-449
abstract
Introduction: Although prostatitis is a common male urinary tract infection, clinical diagnosis of prostatitis is difficult. The developmental mechanism of prostatitis is not yet unraveled which led to the elaboration of various biomarkers. As changes in asparagine-linked-(N-)-glycosylation were observed between healthy volunteers (HV), patients with benign prostate hyperplasia and prostate cancer patients, a difference could exist in biochemical parameters and urinary N-glycosylation between HV and prostatitis patients. We therefore investigated if prostatic protein glycosylation could improve the diagnosis of prostatitis. Materials and methods: Differences in serum and urine biochemical markers and in total urine N-glycosylation profile of prostatic proteins were determined between HV (N = 66) and prostatitis patients (N = 36). Additionally, diagnostic accuracy of significant biochemical markers and changes in N-glycosylation was assessed. Results: Urinary white blood cell (WBC) count enabled discrimination of HV from prostatitis patients (P < 0.001). Urinary bacteria count allowed for discriminating prostatitis patients from HV (P < 0.001). Total amount of biantennary structures (urinary 2A/MA marker) was significantly lower in prostatitis patients compared to HV (P < 0.001). Combining the urinary 2A/MA marker and urinary WBC count resulted in an AUC of 0.79, 95% confidence interval (CI) = (0.70-0.89) which was significantly better than urinary WBC count (AUC = 0.70, 95% CI = [0.59-0.82], P = 0.042) as isolated test. Conclusions: We have demonstrated the diagnostic value of urinary N-glycosylation profiling, which shows great potential as biomarker for prostatitis. Further research is required to unravel the developmental course of prostatic inflammation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ACUTE KIDNEY INJURY, EXPRESSION, INFLAMMATION, ANTIGEN, HYPERPLASIA, UF-1000I, INFECTION, CANCER, DISEASE, TISSUE, diagnostic marker, urinary asparagine-linked glycosylation, prostatitis
journal title
BIOCHEMIA MEDICA
Biochem. Medica.
volume
25
issue
3
pages
439 - 449
Web of Science type
Article
Web of Science id
000362855400014
JCR category
MEDICAL LABORATORY TECHNOLOGY
JCR impact factor
3.051 (2015)
JCR rank
4/30 (2015)
JCR quartile
1 (2015)
ISSN
1330-0962
DOI
10.11613/BM.2015.045
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
7038649
handle
http://hdl.handle.net/1854/LU-7038649
date created
2016-01-15 10:41:20
date last changed
2016-12-19 15:45:43
@article{7038649,
  abstract     = {Introduction: Although prostatitis is a common male urinary tract infection, clinical diagnosis of prostatitis is difficult. The developmental mechanism of prostatitis is not yet unraveled which led to the elaboration of various biomarkers. As changes in asparagine-linked-(N-)-glycosylation were observed between healthy volunteers (HV), patients with benign prostate hyperplasia and prostate cancer patients, a difference could exist in biochemical parameters and urinary N-glycosylation between HV and prostatitis patients. We therefore investigated if prostatic protein glycosylation could improve the diagnosis of prostatitis. 
Materials and methods: Differences in serum and urine biochemical markers and in total urine N-glycosylation profile of prostatic proteins were determined between HV (N = 66) and prostatitis patients (N = 36). Additionally, diagnostic accuracy of significant biochemical markers and changes in N-glycosylation was assessed. 
Results: Urinary white blood cell (WBC) count enabled discrimination of HV from prostatitis patients (P {\textlangle} 0.001). Urinary bacteria count allowed for discriminating prostatitis patients from HV (P {\textlangle} 0.001). Total amount of biantennary structures (urinary 2A/MA marker) was significantly lower in prostatitis patients compared to HV (P {\textlangle} 0.001). Combining the urinary 2A/MA marker and urinary WBC count resulted in an AUC of 0.79, 95\% confidence interval (CI) = (0.70-0.89) which was significantly better than urinary WBC count (AUC = 0.70, 95\% CI = [0.59-0.82], P = 0.042) as isolated test. 
Conclusions: We have demonstrated the diagnostic value of urinary N-glycosylation profiling, which shows great potential as biomarker for prostatitis. Further research is required to unravel the developmental course of prostatic inflammation.},
  author       = {Vermassen, Tijl and Van Praet, Charles and Poelaert, Filip and Lumen, Nicolaas and Decaestecker, Karel and Hoebeke, Piet and Van Belle, Simon and Rottey, Sylvie and Delanghe, Joris},
  issn         = {1330-0962},
  journal      = {BIOCHEMIA MEDICA},
  keyword      = {ACUTE KIDNEY INJURY,EXPRESSION,INFLAMMATION,ANTIGEN,HYPERPLASIA,UF-1000I,INFECTION,CANCER,DISEASE,TISSUE,diagnostic marker,urinary asparagine-linked glycosylation,prostatitis},
  language     = {eng},
  number       = {3},
  pages        = {439--449},
  title        = {Diagnostic accuracy of urinary prostate protein glycosylation profiling in prostatitis diagnosis},
  url          = {http://dx.doi.org/10.11613/BM.2015.045},
  volume       = {25},
  year         = {2015},
}

Chicago
VERMASSEN, TIJL, Charles Van Praet, Filip Poelaert, Nicolaas Lumen, Karel Decaestecker, Piet Hoebeke, Simon Van Belle, Sylvie Rottey, and Joris Delanghe. 2015. “Diagnostic Accuracy of Urinary Prostate Protein Glycosylation Profiling in Prostatitis Diagnosis.” Biochemia Medica 25 (3): 439–449.
APA
VERMASSEN, T., Van Praet, C., Poelaert, F., Lumen, N., Decaestecker, K., Hoebeke, P., Van Belle, S., et al. (2015). Diagnostic accuracy of urinary prostate protein glycosylation profiling in prostatitis diagnosis. BIOCHEMIA MEDICA, 25(3), 439–449.
Vancouver
1.
VERMASSEN T, Van Praet C, Poelaert F, Lumen N, Decaestecker K, Hoebeke P, et al. Diagnostic accuracy of urinary prostate protein glycosylation profiling in prostatitis diagnosis. BIOCHEMIA MEDICA. 2015;25(3):439–49.
MLA
VERMASSEN, TIJL, Charles Van Praet, Filip Poelaert, et al. “Diagnostic Accuracy of Urinary Prostate Protein Glycosylation Profiling in Prostatitis Diagnosis.” BIOCHEMIA MEDICA 25.3 (2015): 439–449. Print.