Advanced search
1 file | 3.19 MB

Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange

Author
Organization
Abstract
The sheep scab mite, Psoroptes ovis, is a major problem in the beef cattle industry, especially in Belgian Blue (BB) cattle. This breed is naturally more predisposed to psoroptic mange but reasons for this high susceptibility remain unknown. Different immune responses could be a potential cause; thus in this study, the cutaneous immune response and in vitro cellular immune response after antigen re-stimulation were examined in naturally infested BB. Cytokine production in the skin and in circulating re-stimulated peripheral blood mononuclear cells (PBMC) demonstrated a mixed pro-inflammatory Th2/Th17 profile, with transcription of IL-4, IL-13, IL-6 and IL-17. Strong IL-17 up-regulation in the skin of BB was associated with an influx of eosinophils and other immune cells, potentially leading towards more severe symptoms. Virtually no changes in cutaneous IFN-gamma transcription were detected, while there was substantial IFN-gamma up-regulation in re-stimulated PBMC from infested and uninfested animals, potentially indicating a role of this pro-inflammatory cytokine in the innate immune response. In Holstein-Friesian (HF) cattle, generally more resistant to P.ovis infection, a largely similar immunologic response was observed. Differences between HF and BB were the lack of cutaneous IL-17 response in infested HF and low transcription levels of IFN-gamma and high IL-10 transcription in re-stimulated PBMC from both infested and uninfested animals. Further research is needed to identify potential cell sources and biological functions for these cytokines and to fully unravel the basis of this different breed susceptibility to P. ovis.
Keywords
CALVES, RESISTANCE, PATHOGENESIS, IMMUNOLOGY, INDUCTION, INFESTATION, DENDRITIC CELLS, STANCHIONED CATTLE, SARCOPTES-SCABIEI, SHEEP SCAB MITE

Downloads

  • naamloos document.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 3.19 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Sarre, Charlotte, Ana González Hernández, Stefanie Van Coppernolle, Grietje Grit, Korneel Grauwet, Frederik Van Meulder, Koen Chiers, Wim Van Den Broeck, Peter Geldhof, and Edwin Claerebout. 2015. “Comparative Immune Responses Against Psoroptes Ovis in Two Cattle Breeds with Different Susceptibility to Mange.” Veterinary Research 46.
APA
Sarre, C., González Hernández, A., Van Coppernolle, S., Grit, G., Grauwet, K., Van Meulder, F., Chiers, K., et al. (2015). Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange. VETERINARY RESEARCH, 46.
Vancouver
1.
Sarre C, González Hernández A, Van Coppernolle S, Grit G, Grauwet K, Van Meulder F, et al. Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange. VETERINARY RESEARCH. 2015;46.
MLA
Sarre, Charlotte, Ana González Hernández, Stefanie Van Coppernolle, et al. “Comparative Immune Responses Against Psoroptes Ovis in Two Cattle Breeds with Different Susceptibility to Mange.” VETERINARY RESEARCH 46 (2015): n. pag. Print.
@article{7037658,
  abstract     = {The sheep scab mite, Psoroptes ovis, is a major problem in the beef cattle industry, especially in Belgian Blue (BB) cattle. This breed is naturally more predisposed to psoroptic mange but reasons for this high susceptibility remain unknown. Different immune responses could be a potential cause; thus in this study, the cutaneous immune response and in vitro cellular immune response after antigen re-stimulation were examined in naturally infested BB. Cytokine production in the skin and in circulating re-stimulated peripheral blood mononuclear cells (PBMC) demonstrated a mixed pro-inflammatory Th2/Th17 profile, with transcription of IL-4, IL-13, IL-6 and IL-17. Strong IL-17 up-regulation in the skin of BB was associated with an influx of eosinophils and other immune cells, potentially leading towards more severe symptoms. Virtually no changes in cutaneous IFN-gamma transcription were detected, while there was substantial IFN-gamma up-regulation in re-stimulated PBMC from infested and uninfested animals, potentially indicating a role of this pro-inflammatory cytokine in the innate immune response. In Holstein-Friesian (HF) cattle, generally more resistant to P.ovis infection, a largely similar immunologic response was observed. Differences between HF and BB were the lack of cutaneous IL-17 response in infested HF and low transcription levels of IFN-gamma and high IL-10 transcription in re-stimulated PBMC from both infested and uninfested animals. Further research is needed to identify potential cell sources and biological functions for these cytokines and to fully unravel the basis of this different breed susceptibility to P. ovis.},
  articleno    = {131},
  author       = {Sarre, Charlotte and Gonz{\'a}lez Hern{\'a}ndez, Ana and Van Coppernolle, Stefanie and Grit, Grietje and Grauwet, Korneel and Van Meulder, Frederik and Chiers, Koen and Van Den Broeck, Wim and Geldhof, Peter and Claerebout, Edwin},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {CALVES,RESISTANCE,PATHOGENESIS,IMMUNOLOGY,INDUCTION,INFESTATION,DENDRITIC CELLS,STANCHIONED CATTLE,SARCOPTES-SCABIEI,SHEEP SCAB MITE},
  language     = {eng},
  pages        = {10},
  title        = {Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange},
  url          = {http://dx.doi.org/10.1186/s13567-015-0277-x},
  volume       = {46},
  year         = {2015},
}

Altmetric
View in Altmetric
Web of Science
Times cited: