GAP activity, but not subcellular targeting, is required for Arabidopsis RanGAP cellular and developmental functions
- Author
- Joanna Boruc (UGent) , Anna HN Griffis, Thushani Rodrigo-Peiris, Xiao Zhou, Bailey Tilford, Daniël Van Damme (UGent) and Iris Meier
- Organization
- Abstract
- The Ran GTPase activating protein (RanGAP) is important to Ran signaling involved in nucleocytoplasmic transport, spindle organization, and postmitotic nuclear assembly. Unlike vertebrate and yeast RanGAP, plant RanGAP has an N-terminal WPP domain, required for nuclear envelope association and several mitotic locations of Arabidopsis thaliana RanGAP1. A double null mutant of the two Arabidopsis RanGAP homologs is gametophyte lethal. Here, we created a series of mutants with various reductions in RanGAP levels by combining a RanGAP1 null allele with different RanGAP2 alleles. As RanGAP level decreases, the severity of developmental phenotypes increases, but nuclear import is unaffected. To dissect whether the GAP activity and/or the subcellular localization of RanGAP are responsible for the observed phenotypes, this series of rangap mutants were transformed with RanGAP1 variants carrying point mutations abolishing the GAP activity and/or the WPP-dependent subcellular localization. The data show that plant development is differentially affected by RanGAP mutant allele combinations of increasing severity and requires the GAP activity of RanGAP, while the subcellular positioning of RanGAP is dispensable. In addition, our results indicate that nucleocytoplasmic trafficking can tolerate both partial depletion of RanGAP and delocalization of RanGAP from the nuclear envelope.
- Keywords
- I-TASSER, GTPASE-ACTIVATING PROTEIN, LEUCINE-RICH REPEAT, PREPROPHASE BAND FORMATION, NUCLEAR-PORE COMPLEX, SPATIAL CONTROL, BINDING PROTEIN, PLANT RANGAP, ROOT, THALIANA
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-7034508
- MLA
- Boruc, Joanna, et al. “GAP Activity, but Not Subcellular Targeting, Is Required for Arabidopsis RanGAP Cellular and Developmental Functions.” PLANT CELL, vol. 27, no. 7, 2015, pp. 1985–98, doi:10.1105/tpc.114.135780.
- APA
- Boruc, J., Griffis, A. H., Rodrigo-Peiris, T., Zhou, X., Tilford, B., Van Damme, D., & Meier, I. (2015). GAP activity, but not subcellular targeting, is required for Arabidopsis RanGAP cellular and developmental functions. PLANT CELL, 27(7), 1985–1998. https://doi.org/10.1105/tpc.114.135780
- Chicago author-date
- Boruc, Joanna, Anna HN Griffis, Thushani Rodrigo-Peiris, Xiao Zhou, Bailey Tilford, Daniël Van Damme, and Iris Meier. 2015. “GAP Activity, but Not Subcellular Targeting, Is Required for Arabidopsis RanGAP Cellular and Developmental Functions.” PLANT CELL 27 (7): 1985–98. https://doi.org/10.1105/tpc.114.135780.
- Chicago author-date (all authors)
- Boruc, Joanna, Anna HN Griffis, Thushani Rodrigo-Peiris, Xiao Zhou, Bailey Tilford, Daniël Van Damme, and Iris Meier. 2015. “GAP Activity, but Not Subcellular Targeting, Is Required for Arabidopsis RanGAP Cellular and Developmental Functions.” PLANT CELL 27 (7): 1985–1998. doi:10.1105/tpc.114.135780.
- Vancouver
- 1.Boruc J, Griffis AH, Rodrigo-Peiris T, Zhou X, Tilford B, Van Damme D, et al. GAP activity, but not subcellular targeting, is required for Arabidopsis RanGAP cellular and developmental functions. PLANT CELL. 2015;27(7):1985–98.
- IEEE
- [1]J. Boruc et al., “GAP activity, but not subcellular targeting, is required for Arabidopsis RanGAP cellular and developmental functions,” PLANT CELL, vol. 27, no. 7, pp. 1985–1998, 2015.
@article{7034508,
abstract = {{The Ran GTPase activating protein (RanGAP) is important to Ran signaling involved in nucleocytoplasmic transport, spindle organization, and postmitotic nuclear assembly. Unlike vertebrate and yeast RanGAP, plant RanGAP has an N-terminal WPP domain, required for nuclear envelope association and several mitotic locations of Arabidopsis thaliana RanGAP1. A double null mutant of the two Arabidopsis RanGAP homologs is gametophyte lethal. Here, we created a series of mutants with various reductions in RanGAP levels by combining a RanGAP1 null allele with different RanGAP2 alleles. As RanGAP level decreases, the severity of developmental phenotypes increases, but nuclear import is unaffected. To dissect whether the GAP activity and/or the subcellular localization of RanGAP are responsible for the observed phenotypes, this series of rangap mutants were transformed with RanGAP1 variants carrying point mutations abolishing the GAP activity and/or the WPP-dependent subcellular localization. The data show that plant development is differentially affected by RanGAP mutant allele combinations of increasing severity and requires the GAP activity of RanGAP, while the subcellular positioning of RanGAP is dispensable. In addition, our results indicate that nucleocytoplasmic trafficking can tolerate both partial depletion of RanGAP and delocalization of RanGAP from the nuclear envelope.}},
author = {{Boruc, Joanna and Griffis, Anna HN and Rodrigo-Peiris, Thushani and Zhou, Xiao and Tilford, Bailey and Van Damme, Daniël and Meier, Iris}},
issn = {{1040-4651}},
journal = {{PLANT CELL}},
keywords = {{I-TASSER,GTPASE-ACTIVATING PROTEIN,LEUCINE-RICH REPEAT,PREPROPHASE BAND FORMATION,NUCLEAR-PORE COMPLEX,SPATIAL CONTROL,BINDING PROTEIN,PLANT RANGAP,ROOT,THALIANA}},
language = {{eng}},
number = {{7}},
pages = {{1985--1998}},
title = {{GAP activity, but not subcellular targeting, is required for Arabidopsis RanGAP cellular and developmental functions}},
url = {{http://dx.doi.org/10.1105/tpc.114.135780}},
volume = {{27}},
year = {{2015}},
}
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