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Quantitative and functional requirements for bioluminescent cancer models

(2016) IN VIVO. 30(1). p.1-11
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Abstract
Background: Bioluminescent cancer models are widely used but detailed quantification of the luciferase signal and functional comparison with a non-transfected control cell line are generally lacking. In the present study, we provide quantitative and functional tests for luciferase-transfected cells. Materials and Methods: We quantified the luciferase expression in BLM and HCT8/E11 transfected cancer cells, and examined the effect of long-term luciferin exposure. The present study also investigated functional differences between parental and transfected cancer cells. Results: Our results showed that quantification of different single-cell-derived populations are superior with droplet digital polymerase chain reaction. Quantification of luciferase protein level and luciferase bioluminescent activity is only useful when there is a significant difference in copy number. Continuous exposure of cell cultures to luciferin leads to inhibitory effects on mitochondrial activity, cell growth and bioluminescence. These inhibitory effects correlate with luciferase copy number. Cell culture and mouse xenograft assays showed no significant functional differences between luciferase-transfected and parental cells. Conclusion: Luciferase-transfected cells should be validated by quantitative and functional assays before starting large-scale experiments.
Keywords
BLM, Bioluminescence, mouse xenograft, cancer model, in vivo imaging, IN-VIVO, TUMOR-GROWTH, LUCIFERASE EXPRESSION, CELLS, METASTASIS, TIME, PROGRESSION, RESISTANCE, EMISSION, KINETICS

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Chicago
Feys, Lynn, Benedicte Descamps, Christian Vanhove, Stefan Vermeulen, Jo Vandesompele, Katrien Vanderheyden, Kathy Messens, Marc Bracke, and Olivier De Wever. 2016. “Quantitative and Functional Requirements for Bioluminescent Cancer Models.” In Vivo 30 (1): 1–11.
APA
Feys, L., Descamps, B., Vanhove, C., Vermeulen, S., Vandesompele, J., Vanderheyden, K., Messens, K., et al. (2016). Quantitative and functional requirements for bioluminescent cancer models. IN VIVO, 30(1), 1–11.
Vancouver
1.
Feys L, Descamps B, Vanhove C, Vermeulen S, Vandesompele J, Vanderheyden K, et al. Quantitative and functional requirements for bioluminescent cancer models. IN VIVO. 2016;30(1):1–11.
MLA
Feys, Lynn, Benedicte Descamps, Christian Vanhove, et al. “Quantitative and Functional Requirements for Bioluminescent Cancer Models.” IN VIVO 30.1 (2016): 1–11. Print.
@article{7025920,
  abstract     = {Background: Bioluminescent cancer models are widely used but detailed quantification of the luciferase signal and functional comparison with a non-transfected control cell line are generally lacking. In the present study, we provide quantitative and functional tests for luciferase-transfected cells.
Materials and Methods: We quantified the luciferase expression in BLM and HCT8/E11 transfected cancer cells, and examined the effect of long-term luciferin exposure. The present study also investigated functional differences between parental and transfected cancer cells.
Results: Our results showed that quantification of different single-cell-derived populations are superior with droplet digital polymerase chain reaction. Quantification of luciferase protein level and luciferase bioluminescent activity is only useful when there is a significant difference in copy number. Continuous exposure of cell cultures to luciferin leads to inhibitory effects on mitochondrial activity, cell growth and bioluminescence. These inhibitory effects correlate with luciferase copy number. Cell culture and mouse xenograft assays showed no significant functional differences between luciferase-transfected and parental cells.
Conclusion: Luciferase-transfected cells should be validated by quantitative and functional assays before starting large-scale experiments.},
  author       = {Feys, Lynn and Descamps, Benedicte and Vanhove, Christian and Vermeulen, Stefan and Vandesompele, Jo and Vanderheyden, Katrien and Messens, Kathy and Bracke, Marc and De Wever, Olivier},
  issn         = {0258-851X},
  journal      = {IN VIVO},
  keyword      = {BLM,Bioluminescence,mouse xenograft,cancer model,in vivo imaging,IN-VIVO,TUMOR-GROWTH,LUCIFERASE EXPRESSION,CELLS,METASTASIS,TIME,PROGRESSION,RESISTANCE,EMISSION,KINETICS},
  language     = {eng},
  number       = {1},
  pages        = {1--11},
  title        = {Quantitative and functional requirements for bioluminescent cancer models},
  url          = {http://iv.iiarjournals.org/content/30/1/1},
  volume       = {30},
  year         = {2016},
}

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