Advanced search
1 file | 825.93 KB

Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-philPLA7 as an anti-biofilm agent in Staphylococcal species

Author
Organization
Abstract
Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The EPS depolymerase (Dpo7) derived from bacteriophage vB_SepiS-philPLA7, was overexpressed in Escherichia coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (>90%) of biofilm-attached cells was obtained with 0.15 mu M of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pretreatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded EPS depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials.
Keywords
EPIDERMIDIS BIOFILMS, WALL TEICHOIC-ACIDS, S. aureus, S. epidermidis, biofilm matrix, biofilm, exopolysaccharide depolymerase, ESCHERICHIA-COLI, IN-VITRO, ACTIVE-SITE, AUREUS, BACTERIOPHAGE, INFECTIONS, VIRULENCE, SURFACE

Downloads

  • fmicb-06-01315.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 825.93 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Gutiérrez, Diana, Yves Briers, Lorena Rodríguez-Rubio, Beatriz Martínez, Ana Rodríguez, Rob Lavigne, and Pilar García. 2015. “Role of the Pre-neck Appendage Protein (Dpo7) from Phage vB_SepiS-philPLA7 as an Anti-biofilm Agent in Staphylococcal Species.” Frontiers in Microbiology 6.
APA
Gutiérrez, D., Briers, Y., Rodríguez-Rubio, L., Martínez, B., Rodríguez, A., Lavigne, R., & García, P. (2015). Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-philPLA7 as an anti-biofilm agent in Staphylococcal species. FRONTIERS IN MICROBIOLOGY, 6.
Vancouver
1.
Gutiérrez D, Briers Y, Rodríguez-Rubio L, Martínez B, Rodríguez A, Lavigne R, et al. Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-philPLA7 as an anti-biofilm agent in Staphylococcal species. FRONTIERS IN MICROBIOLOGY. 2015;6.
MLA
Gutiérrez, Diana, Yves Briers, Lorena Rodríguez-Rubio, et al. “Role of the Pre-neck Appendage Protein (Dpo7) from Phage vB_SepiS-philPLA7 as an Anti-biofilm Agent in Staphylococcal Species.” FRONTIERS IN MICROBIOLOGY 6 (2015): n. pag. Print.
@article{7025098,
  abstract     = {Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The EPS depolymerase (Dpo7) derived from bacteriophage vB\_SepiS-philPLA7, was overexpressed in Escherichia coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal ({\textrangle}90\%) of biofilm-attached cells was obtained with 0.15 mu M of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pretreatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85\% in the 67\% of the tested strains. This study supports the use of phage-encoded EPS depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials.},
  articleno    = {1315},
  author       = {Guti{\'e}rrez, Diana and Briers, Yves and Rodr{\'i}guez-Rubio, Lorena and Mart{\'i}nez, Beatriz and Rodr{\'i}guez, Ana and Lavigne, Rob and Garc{\'i}a, Pilar},
  issn         = {1664-302X},
  journal      = {FRONTIERS IN MICROBIOLOGY},
  keyword      = {EPIDERMIDIS BIOFILMS,WALL TEICHOIC-ACIDS,S. aureus,S. epidermidis,biofilm matrix,biofilm,exopolysaccharide depolymerase,ESCHERICHIA-COLI,IN-VITRO,ACTIVE-SITE,AUREUS,BACTERIOPHAGE,INFECTIONS,VIRULENCE,SURFACE},
  language     = {eng},
  pages        = {10},
  title        = {Role of the pre-neck appendage protein (Dpo7) from phage vB\_SepiS-philPLA7 as an anti-biofilm agent in Staphylococcal species},
  url          = {http://dx.doi.org/10.3389/fmicb.2015.01315},
  volume       = {6},
  year         = {2015},
}

Altmetric
View in Altmetric
Web of Science
Times cited: