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The equine embryo influences immune related gene expression in the oviduct

Katrien Smits (UGent) , Dieter De Coninck (UGent) , Filip Van Nieuwerburgh (UGent) , Jan Govaere (UGent) , Mario Van Poucke (UGent) , Luc Peelman (UGent) , Dieter Deforce (UGent) and Ann Van Soom (UGent)
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Abstract
Although the equine oviduct clearly affects early embryo development and the selective transport of equine embryos through the oviduct indicates a reciprocal interaction, the influence of the embryo on gene expression in the oviduct remains to be determined in the horse. The aim of this study was to examine this by means of RNA sequencing. Four days after ovulation, epithelial cells ipsilateral and contralateral to the ovulation side from five cyclic and five pregnant mares were collected from the oviduct. RNA was extracted, samples were sequenced, and data analysis was performed to determine differentially expressed genes (DEGs) (P value <= 0.05 and absolute fold change >= 2) and to provide functional interpretation. A total of 10 743 transcripts were identified and 253 genes were found to be upregulated and 108 to be downregulated in the pregnant ipsilateral oviduct when compared to the cyclic ipsilateral oviduct. Comparison of the ipsilateral and the contralateral oviduct indicated 164 DEGs in pregnant mares and 77 DEGs in cyclic mares. Enriched functional categories were detected only in the comparison of pregnant and cyclic ipsilateral oviducts and showed that the equine embryo affects the expression of immune response-related genes in the oviduct, with marked upregulation of interferon-associated genes. This research represents the foundation for further assessment of the role of specific genes in the early embryo-maternal dialogue of the horse.
Keywords
horse, RNA sequencing, gene expression, oviduct, embryo, MESSENGER-RNA EXPRESSION, BOVINE EMBRYOS, MATERNAL RECOGNITION, UTERINE ENVIRONMENT, IN-VIVO, PREGNANCY, HORSE, TRANSCRIPTOME, PORCINE, CULTURE

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MLA
Smits, Katrien et al. “The Equine Embryo Influences Immune Related Gene Expression in the Oviduct.” BIOLOGY OF REPRODUCTION 94.2 (2016): n. pag. Print.
APA
Smits, K., De Coninck, D., Van Nieuwerburgh, F., Govaere, J., Van Poucke, M., Peelman, L., Deforce, D., et al. (2016). The equine embryo influences immune related gene expression in the oviduct. BIOLOGY OF REPRODUCTION, 94(2).
Chicago author-date
Smits, Katrien, Dieter De Coninck, Filip Van Nieuwerburgh, Jan Govaere, Mario Van Poucke, Luc Peelman, Dieter Deforce, and Ann Van Soom. 2016. “The Equine Embryo Influences Immune Related Gene Expression in the Oviduct.” Biology of Reproduction 94 (2).
Chicago author-date (all authors)
Smits, Katrien, Dieter De Coninck, Filip Van Nieuwerburgh, Jan Govaere, Mario Van Poucke, Luc Peelman, Dieter Deforce, and Ann Van Soom. 2016. “The Equine Embryo Influences Immune Related Gene Expression in the Oviduct.” Biology of Reproduction 94 (2).
Vancouver
1.
Smits K, De Coninck D, Van Nieuwerburgh F, Govaere J, Van Poucke M, Peelman L, et al. The equine embryo influences immune related gene expression in the oviduct. BIOLOGY OF REPRODUCTION. 2016;94(2).
IEEE
[1]
K. Smits et al., “The equine embryo influences immune related gene expression in the oviduct,” BIOLOGY OF REPRODUCTION, vol. 94, no. 2, 2016.
@article{7024767,
  abstract     = {Although the equine oviduct clearly affects early embryo development and the selective transport of equine embryos through the oviduct indicates a reciprocal interaction, the influence of the embryo on gene expression in the oviduct remains to be determined in the horse. The aim of this study was to examine this by means of RNA sequencing. Four days after ovulation, epithelial cells ipsilateral and contralateral to the ovulation side from five cyclic and five pregnant mares were collected from the oviduct. RNA was extracted, samples were sequenced, and data analysis was performed to determine differentially expressed genes (DEGs) (P value <= 0.05 and absolute fold change >= 2) and to provide functional interpretation. A total of 10 743 transcripts were identified and 253 genes were found to be upregulated and 108 to be downregulated in the pregnant ipsilateral oviduct when compared to the cyclic ipsilateral oviduct. Comparison of the ipsilateral and the contralateral oviduct indicated 164 DEGs in pregnant mares and 77 DEGs in cyclic mares. Enriched functional categories were detected only in the comparison of pregnant and cyclic ipsilateral oviducts and showed that the equine embryo affects the expression of immune response-related genes in the oviduct, with marked upregulation of interferon-associated genes. This research represents the foundation for further assessment of the role of specific genes in the early embryo-maternal dialogue of the horse.},
  articleno    = {36},
  author       = {Smits, Katrien and De Coninck, Dieter and Van Nieuwerburgh, Filip and Govaere, Jan and Van Poucke, Mario and Peelman, Luc and Deforce, Dieter and Van Soom, Ann},
  issn         = {0006-3363},
  journal      = {BIOLOGY OF REPRODUCTION},
  keywords     = {horse,RNA sequencing,gene expression,oviduct,embryo,MESSENGER-RNA EXPRESSION,BOVINE EMBRYOS,MATERNAL RECOGNITION,UTERINE ENVIRONMENT,IN-VIVO,PREGNANCY,HORSE,TRANSCRIPTOME,PORCINE,CULTURE},
  language     = {eng},
  number       = {2},
  pages        = {8},
  title        = {The equine embryo influences immune related gene expression in the oviduct},
  url          = {http://dx.doi.org/10.1095/biolreprod.115.136432},
  volume       = {94},
  year         = {2016},
}

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