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Short communication: Subtyping of Staphylococcus haemolyticus isolates from milk and corresponding teat apices to verify the potential teat-skin origin of intramammary infections in dairy cows

(2015) JOURNAL OF DAIRY SCIENCE. 98(11). p.7893-7898
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Abstract
Coagulase-negative staphylococci (CNS) are a major cause of intramammary infections (IMI) in dairy cows and they colonize the teat skin. Staphylococcus haemolyticus, one of the more common CNS, has been identified as a highly versatile opportunistic species. The aim of the present study was to gain better insight into the adaptation of S. haemolyticus subtypes to the udder ecosystem with respect to IMI development. During a longitudinal observational study conducted over 13 mo on 6 Flemish dairy herds, S. haemolyticus isolates were recovered from milk and teat apices. A total of 44 S. haemolyticus isolates originating from milk (24 isolates) and teat apices (20 isolates) of 6 selected udder quarters were singled out and analyzed using a combined methodology of (GTG)(5)-PCR and amplified fragment length polymorphism (AFLP) fingerprinting to determine intraspecies differences. Combining both fingerprinting methods, 4 S. haemolyticus subtypes were obtained (I to IV). Subtypes I, II, and IV were recovered from both milk and teat apex samples and were found to be associated with persisting IMI. Subtype III, not apparently related to IMI, was isolated solely from teat apices and not from milk. In general, S. haemolyticus subtypes found in milk from infected quarters could be recovered from the corresponding teat apices, although the latter could be colonized with up to 3 different subtypes. Comparing subtypes from milk and teat apices indicates that the IMI-causing agent likely originates from the teat skin.
Keywords
bovine mastitis, teat apex, SOMATIC-CELL COUNT, COAGULASE-NEGATIVE STAPHYLOCOCCI, CATTLE, Staphylococcus haemolyticus, (GTG)(5)-PCR fingerprinting, amplified fragment length polymorphism (AFLP) fingerprinting

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Citation

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MLA
Leroy, Frédéric et al. “Short Communication: Subtyping of Staphylococcus Haemolyticus Isolates from Milk and Corresponding Teat Apices to Verify the Potential Teat-skin Origin of Intramammary Infections in Dairy Cows.” JOURNAL OF DAIRY SCIENCE 98.11 (2015): 7893–7898. Print.
APA
Leroy, Frédéric, Van Coillie, E., Braem, G., Piessens, V., Verbist, B., De Vuyst, L., & De Vliegher, S. (2015). Short communication: Subtyping of Staphylococcus haemolyticus isolates from milk and corresponding teat apices to verify the potential teat-skin origin of intramammary infections in dairy cows. JOURNAL OF DAIRY SCIENCE, 98(11), 7893–7898.
Chicago author-date
Leroy, Frédéric, Els Van Coillie, Gorik Braem, Veerle Piessens, Bert Verbist, Luc De Vuyst, and Sarne De Vliegher. 2015. “Short Communication: Subtyping of Staphylococcus Haemolyticus Isolates from Milk and Corresponding Teat Apices to Verify the Potential Teat-skin Origin of Intramammary Infections in Dairy Cows.” Journal of Dairy Science 98 (11): 7893–7898.
Chicago author-date (all authors)
Leroy, Frédéric, Els Van Coillie, Gorik Braem, Veerle Piessens, Bert Verbist, Luc De Vuyst, and Sarne De Vliegher. 2015. “Short Communication: Subtyping of Staphylococcus Haemolyticus Isolates from Milk and Corresponding Teat Apices to Verify the Potential Teat-skin Origin of Intramammary Infections in Dairy Cows.” Journal of Dairy Science 98 (11): 7893–7898.
Vancouver
1.
Leroy F, Van Coillie E, Braem G, Piessens V, Verbist B, De Vuyst L, et al. Short communication: Subtyping of Staphylococcus haemolyticus isolates from milk and corresponding teat apices to verify the potential teat-skin origin of intramammary infections in dairy cows. JOURNAL OF DAIRY SCIENCE. 2015;98(11):7893–8.
IEEE
[1]
F. Leroy et al., “Short communication: Subtyping of Staphylococcus haemolyticus isolates from milk and corresponding teat apices to verify the potential teat-skin origin of intramammary infections in dairy cows,” JOURNAL OF DAIRY SCIENCE, vol. 98, no. 11, pp. 7893–7898, 2015.
@article{7022307,
  abstract     = {Coagulase-negative staphylococci (CNS) are a major cause of intramammary infections (IMI) in dairy cows and they colonize the teat skin. Staphylococcus haemolyticus, one of the more common CNS, has been identified as a highly versatile opportunistic species. The aim of the present study was to gain better insight into the adaptation of S. haemolyticus subtypes to the udder ecosystem with respect to IMI development. During a longitudinal observational study conducted over 13 mo on 6 Flemish dairy herds, S. haemolyticus isolates were recovered from milk and teat apices. A total of 44 S. haemolyticus isolates originating from milk (24 isolates) and teat apices (20 isolates) of 6 selected udder quarters were singled out and analyzed using a combined methodology of (GTG)(5)-PCR and amplified fragment length polymorphism (AFLP) fingerprinting to determine intraspecies differences. Combining both fingerprinting methods, 4 S. haemolyticus subtypes were obtained (I to IV). Subtypes I, II, and IV were recovered from both milk and teat apex samples and were found to be associated with persisting IMI. Subtype III, not apparently related to IMI, was isolated solely from teat apices and not from milk. In general, S. haemolyticus subtypes found in milk from infected quarters could be recovered from the corresponding teat apices, although the latter could be colonized with up to 3 different subtypes. Comparing subtypes from milk and teat apices indicates that the IMI-causing agent likely originates from the teat skin.},
  author       = {Leroy, Frédéric and Van Coillie, Els and Braem, Gorik and Piessens, Veerle and Verbist, Bert and De Vuyst, Luc and De Vliegher, Sarne},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keywords     = {bovine mastitis,teat apex,SOMATIC-CELL COUNT,COAGULASE-NEGATIVE STAPHYLOCOCCI,CATTLE,Staphylococcus haemolyticus,(GTG)(5)-PCR fingerprinting,amplified fragment length polymorphism (AFLP) fingerprinting},
  language     = {eng},
  number       = {11},
  pages        = {7893--7898},
  title        = {Short communication: Subtyping of Staphylococcus haemolyticus isolates from milk and corresponding teat apices to verify the potential teat-skin origin of intramammary infections in dairy cows},
  url          = {http://dx.doi.org/10.3168/jds.2015-9415},
  volume       = {98},
  year         = {2015},
}

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