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In vitro prediction of human intestinal absorption and blood-brain barrier partitioning: development of a lipid analog for micellar liquid chromatography

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Abstract
Over the past decades, several in vitro methods have been tested for their ability to predict either human intestinal absorption (HIA) or penetration across the blood-brain barrier (BBB) of drugs. Micellar liquid chromatography (MLC) has been a successful approach for retention time measurements of drugs to establish models together with other molecular descriptors. Thus far, MLC approaches have only made use of commercial surfactants such as sodium dodecyl sulfate (SDS) and polyoxyethylene (23) lauryl ether (Brij35), which are not representative for the phospholipids present in human membranes. Miltefosine, a phosphocholine-based lipid, is presented here as an alternative surfactant for MLC measurements. By using the obtained retention factors and several computed descriptors for a set of 48 compounds, two models were constructed: one for the prediction of HIA and another for the prediction of penetration across the BBB expressed as log BB. All data were correlated to experimental HIA and log BB values, and the performance of the models was evaluated. Log BB prediction performed better than HIA prediction, although HIA prediction was also improved a lot (from 0.5530 to 0.7175) compared to in silico predicted HIA values.
Keywords
Micellar liquid chromatography, Human intestinal absorption, Blood-brain barrier, SILICO PREDICTION, DRUG DISCOVERY, PERMEABILITY, HEXADECYLPHOSPHOCHOLINE, TRANSPORT, MODELS, QUANTITATIVE RETENTION, ADME EVALUATION, MOLECULAR-WEIGHT, CENTRAL-NERVOUS-SYSTEM, Modeling, Miltefosine

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Chicago
De Vrieze, Mike, Pieter Janssens, Roman Szucs, Johan Van der Eycken, and Frederic Lynen. 2015. “In Vitro Prediction of Human Intestinal Absorption and Blood-brain Barrier Partitioning: Development of a Lipid Analog for Micellar Liquid Chromatography.” Analytical and Bioanalytical Chemistry 407 (24): 7453–7466.
APA
De Vrieze, M., Janssens, P., Szucs, R., Van der Eycken, J., & Lynen, F. (2015). In vitro prediction of human intestinal absorption and blood-brain barrier partitioning: development of a lipid analog for micellar liquid chromatography. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 407(24), 7453–7466.
Vancouver
1.
De Vrieze M, Janssens P, Szucs R, Van der Eycken J, Lynen F. In vitro prediction of human intestinal absorption and blood-brain barrier partitioning: development of a lipid analog for micellar liquid chromatography. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2015;407(24):7453–66.
MLA
De Vrieze, Mike et al. “In Vitro Prediction of Human Intestinal Absorption and Blood-brain Barrier Partitioning: Development of a Lipid Analog for Micellar Liquid Chromatography.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 407.24 (2015): 7453–7466. Print.
@article{7020015,
  abstract     = {Over the past decades, several in vitro methods have been tested for their ability to predict either human intestinal absorption (HIA) or penetration across the blood-brain barrier (BBB) of drugs. Micellar liquid chromatography (MLC) has been a successful approach for retention time measurements of drugs to establish models together with other molecular descriptors. Thus far, MLC approaches have only made use of commercial surfactants such as sodium dodecyl sulfate (SDS) and polyoxyethylene (23) lauryl ether (Brij35), which are not representative for the phospholipids present in human membranes. Miltefosine, a phosphocholine-based lipid, is presented here as an alternative surfactant for MLC measurements. By using the obtained retention factors and several computed descriptors for a set of 48 compounds, two models were constructed: one for the prediction of HIA and another for the prediction of penetration across the BBB expressed as log BB. All data were correlated to experimental HIA and log BB values, and the performance of the models was evaluated. Log BB prediction performed better than HIA prediction, although HIA prediction was also improved a lot (from 0.5530 to 0.7175) compared to in silico predicted HIA values.},
  author       = {De Vrieze, Mike and Janssens, Pieter and Szucs, Roman and Van der Eycken, Johan and Lynen, Frederic},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keywords     = {Micellar liquid chromatography,Human intestinal absorption,Blood-brain barrier,SILICO PREDICTION,DRUG DISCOVERY,PERMEABILITY,HEXADECYLPHOSPHOCHOLINE,TRANSPORT,MODELS,QUANTITATIVE RETENTION,ADME EVALUATION,MOLECULAR-WEIGHT,CENTRAL-NERVOUS-SYSTEM,Modeling,Miltefosine},
  language     = {eng},
  number       = {24},
  pages        = {7453--7466},
  title        = {In vitro prediction of human intestinal absorption and blood-brain barrier partitioning: development of a lipid analog for micellar liquid chromatography},
  url          = {http://dx.doi.org/10.1007/s00216-015-8911-z},
  volume       = {407},
  year         = {2015},
}

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