Advanced search
1 file | 3.78 MB Add to list

Viral small-RNA analysis of Bombyx mori larval midgut during persistent and pathogenic cytoplasmic polyhedrosis virus infection

(2015) JOURNAL OF VIROLOGY. 89(22). p.11473-11486
Author
Organization
Abstract
The lepidopteran innate immune response against RNA viruses remains poorly understood, while in other insects several studies have highlighted an essential role for the exo-RNAi pathway in combating viral infection. Here, by using deep-sequencing technology for viral small-RNA (vsRNA) assessment, we provide evidence that exo-RNAi is operative in the silkworm Bombyx mori against both persistent and pathogenic infection of B. mori cytoplasmic polyhedrosis virus (BmCPV) which is characterized by a segmented double-stranded RNA (dsRNA) genome. Further, we show that Dicer-2 predominantly targets viral dsRNA and produces 20-nucleotide (nt) vsRNAs, whereas an additional pathway is responsive to viral mRNA derived from segment 10. Importantly, vsRNA distributions, which define specific hot and cold spot profiles for each viral segment, to a considerable degree overlap between Dicer-2-related (19 to 21 nt) and Dicer-2-unrelated vsRNAs, suggesting a common origin for these profiles. We found a degenerate motif significantly enriched at the cut sites of vsRNAs of various lengths which link an unknown RNase to the origins of vsRNAs biogenesis and distribution. Accordingly, the indicated RNase activity may be an important early factor for the host's antiviral defense in Lepidoptera. IMPORTANCE : This work contributes to the elucidation of the lepidopteran antiviral response against infection of segmented double-stranded RNA (dsRNA) virus (CPV; Reoviridae) and highlights the importance of viral small-RNA (vsRNA) analysis for getting insights into host-pathogen interactions. Three vsRNA pathways are implicated in antiviral defense. For dsRNA, two pathways are proposed, either based on Dicer-2 cleavage to generate 20-nucleotide vsRNAs or based on the activity of an uncharacterized endo-RNase that cleaves the viral RNA substrate at a degenerate motif. The analysis also indicates the existence of a degradation pathway that targets the positive strand of segment 10.
Keywords
INTERFERENCE, MOSQUITO CELLS, REPLICATION, DROSOPHILA, EXPRESSION, IMMUNITY, POPULATIONS, BACULOVIRUS, DISCOVERY, SEQUENCES

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.78 MB

Citation

Please use this url to cite or link to this publication:

MLA
Zografidis, Aris et al. “Viral small-RNA Analysis of Bombyx Mori Larval Midgut During Persistent and Pathogenic Cytoplasmic Polyhedrosis Virus Infection.” JOURNAL OF VIROLOGY 89.22 (2015): 11473–11486. Print.
APA
Zografidis, A., Van Nieuwerburgh, F., Kolliopoulou, A., Apostolou-Karampelis, K., Head, S. R., Deforce, D., Smagghe, G., et al. (2015). Viral small-RNA analysis of Bombyx mori larval midgut during persistent and pathogenic cytoplasmic polyhedrosis virus infection. JOURNAL OF VIROLOGY, 89(22), 11473–11486.
Chicago author-date
Zografidis, Aris, Filip Van Nieuwerburgh, Anna Kolliopoulou, Konstantinos Apostolou-Karampelis, Steven R Head, Dieter Deforce, Guy Smagghe, and Luc Swevers. 2015. “Viral small-RNA Analysis of Bombyx Mori Larval Midgut During Persistent and Pathogenic Cytoplasmic Polyhedrosis Virus Infection.” Journal of Virology 89 (22): 11473–11486.
Chicago author-date (all authors)
Zografidis, Aris, Filip Van Nieuwerburgh, Anna Kolliopoulou, Konstantinos Apostolou-Karampelis, Steven R Head, Dieter Deforce, Guy Smagghe, and Luc Swevers. 2015. “Viral small-RNA Analysis of Bombyx Mori Larval Midgut During Persistent and Pathogenic Cytoplasmic Polyhedrosis Virus Infection.” Journal of Virology 89 (22): 11473–11486.
Vancouver
1.
Zografidis A, Van Nieuwerburgh F, Kolliopoulou A, Apostolou-Karampelis K, Head SR, Deforce D, et al. Viral small-RNA analysis of Bombyx mori larval midgut during persistent and pathogenic cytoplasmic polyhedrosis virus infection. JOURNAL OF VIROLOGY. 2015;89(22):11473–86.
IEEE
[1]
A. Zografidis et al., “Viral small-RNA analysis of Bombyx mori larval midgut during persistent and pathogenic cytoplasmic polyhedrosis virus infection,” JOURNAL OF VIROLOGY, vol. 89, no. 22, pp. 11473–11486, 2015.
@article{7001191,
  abstract     = {The lepidopteran innate immune response against RNA viruses remains poorly understood, while in other insects several studies have highlighted an essential role for the exo-RNAi pathway in combating viral infection. Here, by using deep-sequencing technology for viral small-RNA (vsRNA) assessment, we provide evidence that exo-RNAi is operative in the silkworm Bombyx mori against both persistent and pathogenic infection of B. mori cytoplasmic polyhedrosis virus (BmCPV) which is characterized by a segmented double-stranded RNA (dsRNA) genome. Further, we show that Dicer-2 predominantly targets viral dsRNA and produces 20-nucleotide (nt) vsRNAs, whereas an additional pathway is responsive to viral mRNA derived from segment 10. Importantly, vsRNA distributions, which define specific hot and cold spot profiles for each viral segment, to a considerable degree overlap between Dicer-2-related (19 to 21 nt) and Dicer-2-unrelated vsRNAs, suggesting a common origin for these profiles. We found a degenerate motif significantly enriched at the cut sites of vsRNAs of various lengths which link an unknown RNase to the origins of vsRNAs biogenesis and distribution. Accordingly, the indicated RNase activity may be an important early factor for the host's antiviral defense in Lepidoptera. 
IMPORTANCE : This work contributes to the elucidation of the lepidopteran antiviral response against infection of segmented double-stranded RNA (dsRNA) virus (CPV; Reoviridae) and highlights the importance of viral small-RNA (vsRNA) analysis for getting insights into host-pathogen interactions. Three vsRNA pathways are implicated in antiviral defense. For dsRNA, two pathways are proposed, either based on Dicer-2 cleavage to generate 20-nucleotide vsRNAs or based on the activity of an uncharacterized endo-RNase that cleaves the viral RNA substrate at a degenerate motif. The analysis also indicates the existence of a degradation pathway that targets the positive strand of segment 10.},
  author       = {Zografidis, Aris and Van Nieuwerburgh, Filip and Kolliopoulou, Anna and Apostolou-Karampelis, Konstantinos and Head, Steven R and Deforce, Dieter and Smagghe, Guy and Swevers, Luc},
  issn         = {0022-538X},
  journal      = {JOURNAL OF VIROLOGY},
  keywords     = {INTERFERENCE,MOSQUITO CELLS,REPLICATION,DROSOPHILA,EXPRESSION,IMMUNITY,POPULATIONS,BACULOVIRUS,DISCOVERY,SEQUENCES},
  language     = {eng},
  number       = {22},
  pages        = {11473--11486},
  title        = {Viral small-RNA analysis of Bombyx mori larval midgut during persistent and pathogenic cytoplasmic polyhedrosis virus infection},
  url          = {http://dx.doi.org/10.1128/JVI.01695-15},
  volume       = {89},
  year         = {2015},
}

Altmetric
View in Altmetric
Web of Science
Times cited: