Ghent University Academic Bibliography

Advanced

PLCζ disruption with complete fertilization failure in normozoospermia

Mercè Durban, Montserrat Barragan, Marta Colodron, Minerva Ferrer Buitrago UGent, Petra De Sutter UGent, Björn Heindryckx UGent, Valérie Vernaeve and Rita Vassena (2015) JOURNAL OF ASSISTED REPRODUCTION AND GENETICS. 32(6). p.879-886
abstract
Purpose: Intracytoplasmic sperm injection (ICSI) is widely used to achieve fertilization in the presence of severe male factor, resulting in high fertilization rates. Nevertheless, 1–3 % of couples experience complete fertilization failure after ICSI.When a male factor is identified, assisted oocyte activation (AOA) can help overcome fertilization failures. The objective of this study is to describe a case of repeated complete fertilization failures after ICSI with donor oocytes, and to investigate the molecular and functional aspects of phospholipase C zeta (PLCζ) protein in the patient semen. Methods: The patient was a normozoospermic male who had previously fathered, through natural conception, four children by a different partner. Molecular and functional analysis of sperm-specific PLCζ in the patient and control samples by means of gene sequencing, immunocytochemistry, Western blot, mouse oocyte activation test (MOAT), and mouse oocyte calcium analysis (MOCA) were used. Results: PLCζ expression levels and distribution were significantly disrupted, although MOAT and MOCA did not indicate a decrease in activation ability. Conclusions: Normozoospermic males can have disrupted expression and distribution of PLCζ, and reduced activation ability after ICSI in human oocytes, despite their normal activation potential in functional testing using mouse oocytes. Discrepancy among molecular and functional data might exist, as mutations in the gene sequence may not be the only cause of alteration in PLCζ protein related to activation failures.
Please use this url to cite or link to this publication:
author
organization
alternative title
PLC zeta disruption with complete fertilization failure in normozoospermia
year
type
journalArticle (original)
publication status
published
subject
keyword
Assisted oocyte activation, PHOSPHOLIPASE-C-ZETA, Fertilization failure, INTRACYTOPLASMIC SPERM INJECTION, PLC zeta, Normozoospermia, ICSI, ASSISTED OOCYTE ACTIVATION, MALE FACTOR INFERTILITY, FAILED FERTILIZATION, SUCCESSFUL PREGNANCY, CALCIUM IONOPHORE, BINDING-PROTEIN, ICSI, SPERMATOZOA
journal title
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
J. Assist. Reprod. Genet.
volume
32
issue
6
pages
879 - 886
Web of Science type
Article
Web of Science id
000357451500005
JCR category
OBSTETRICS & GYNECOLOGY
JCR impact factor
1.858 (2015)
JCR rank
40/80 (2015)
JCR quartile
2 (2015)
ISSN
1058-0468
DOI
10.1007/s10815-015-0496-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
7000749
handle
http://hdl.handle.net/1854/LU-7000749
date created
2015-12-03 14:03:32
date last changed
2016-12-19 15:39:50
@article{7000749,
  abstract     = {Purpose: Intracytoplasmic sperm injection (ICSI) is widely used to achieve fertilization in the presence of severe male factor, resulting in high fertilization rates. Nevertheless, 1--3 \% of couples experience complete fertilization failure after ICSI.When a male factor is identified, assisted oocyte activation (AOA) can help overcome fertilization failures. The objective of this study is to describe a case of repeated complete fertilization failures after ICSI with donor oocytes, and to investigate the molecular and functional aspects of phospholipase C zeta (PLC\ensuremath{\zeta}) protein in the patient semen.
Methods: The patient was a normozoospermic male who had previously fathered, through natural conception, four children by a different partner. Molecular and functional analysis of sperm-specific PLC\ensuremath{\zeta} in the patient and control samples by means of gene sequencing, immunocytochemistry, Western blot, mouse oocyte activation test (MOAT), and mouse oocyte calcium analysis (MOCA) were used.
Results: PLC\ensuremath{\zeta} expression levels and distribution were significantly disrupted, although MOAT and MOCA did not indicate a decrease in activation ability.
Conclusions: Normozoospermic males can have disrupted expression and distribution of PLC\ensuremath{\zeta}, and reduced activation ability after ICSI in human oocytes, despite their normal activation potential in functional testing using mouse oocytes. Discrepancy among molecular and functional data might exist, as mutations in the gene sequence may not be the only cause of alteration in PLC\ensuremath{\zeta} protein related to activation failures.},
  author       = {Durban, Merc{\`e} and Barragan, Montserrat and Colodron, Marta and Ferrer Buitrago, Minerva and De Sutter, Petra and Heindryckx, Bj{\"o}rn and Vernaeve, Val{\'e}rie and Vassena, Rita},
  issn         = {1058-0468},
  journal      = {JOURNAL OF ASSISTED REPRODUCTION AND GENETICS},
  keyword      = {Assisted oocyte activation,PHOSPHOLIPASE-C-ZETA,Fertilization failure,INTRACYTOPLASMIC SPERM INJECTION,PLC zeta,Normozoospermia,ICSI,ASSISTED OOCYTE ACTIVATION,MALE FACTOR INFERTILITY,FAILED FERTILIZATION,SUCCESSFUL PREGNANCY,CALCIUM IONOPHORE,BINDING-PROTEIN,ICSI,SPERMATOZOA},
  language     = {eng},
  number       = {6},
  pages        = {879--886},
  title        = {PLC\ensuremath{\zeta} disruption with complete fertilization failure in normozoospermia},
  url          = {http://dx.doi.org/10.1007/s10815-015-0496-0},
  volume       = {32},
  year         = {2015},
}

Chicago
Durban, Mercè, Montserrat Barragan, Marta Colodron, Minerva Ferrer Buitrago, Petra De Sutter, Björn Heindryckx, Valérie Vernaeve, and Rita Vassena. 2015. “PLCζ Disruption with Complete Fertilization Failure in Normozoospermia.” Journal of Assisted Reproduction and Genetics 32 (6): 879–886.
APA
Durban, M., Barragan, M., Colodron, M., Ferrer Buitrago, M., De Sutter, P., Heindryckx, B., Vernaeve, V., et al. (2015). PLCζ disruption with complete fertilization failure in normozoospermia. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 32(6), 879–886.
Vancouver
1.
Durban M, Barragan M, Colodron M, Ferrer Buitrago M, De Sutter P, Heindryckx B, et al. PLCζ disruption with complete fertilization failure in normozoospermia. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS. 2015;32(6):879–86.
MLA
Durban, Mercè, Montserrat Barragan, Marta Colodron, et al. “PLCζ Disruption with Complete Fertilization Failure in Normozoospermia.” JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 32.6 (2015): 879–886. Print.