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Lessons from the first international proficiency test for the detection of spores from the honey bee pathogen Paenibacillus larvae

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Abstract
We report on the first proficiency test in the context of honey bee disease testing on a broad international scale. Honey samples were distributed to 12 participating laboratories for isolation and identification of spores of the etiological agent of American foulbrood, Paenibacillus larvae. Of the 11 laboratories responding to this proficiency testing event, only 6 (54%) provided data that were in full agreement with the previously determined status of the honey samples; eight (72%) laboratories fell within the threshold for qualification as defined a priori in this proficiency test (=level of agreement of at least 90%). Some lessons can now be drawn from the organizational point of view and will certainly improve new initiatives to be taken.
Keywords
AMERICAN FOULBROOD DISEASE, GENOTYPES, VIRULENCE, PNEUMONIAE, DIAGNOSIS, RESISTANCE, LABORATORIES, SUBSP LARVAE, BACILLUS-LARVAE, APIS-MELLIFERA

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Citation

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MLA
de Graaf, Dirk et al. “Lessons from the First International Proficiency Test for the Detection of Spores from the Honey Bee Pathogen Paenibacillus Larvae.” ACCREDITATION AND QUALITY ASSURANCE 14.5 (2009): 273–276. Print.
APA
de Graaf, D., Ritter, W., Jacobs, F., & Brunain, M. (2009). Lessons from the first international proficiency test for the detection of spores from the honey bee pathogen Paenibacillus larvae. ACCREDITATION AND QUALITY ASSURANCE, 14(5), 273–276.
Chicago author-date
de Graaf, Dirk, Wolfgang Ritter, Franciscus Jacobs, and Marleen Brunain. 2009. “Lessons from the First International Proficiency Test for the Detection of Spores from the Honey Bee Pathogen Paenibacillus Larvae.” Accreditation and Quality Assurance 14 (5): 273–276.
Chicago author-date (all authors)
de Graaf, Dirk, Wolfgang Ritter, Franciscus Jacobs, and Marleen Brunain. 2009. “Lessons from the First International Proficiency Test for the Detection of Spores from the Honey Bee Pathogen Paenibacillus Larvae.” Accreditation and Quality Assurance 14 (5): 273–276.
Vancouver
1.
de Graaf D, Ritter W, Jacobs F, Brunain M. Lessons from the first international proficiency test for the detection of spores from the honey bee pathogen Paenibacillus larvae. ACCREDITATION AND QUALITY ASSURANCE. NEW YORK, USA: Springer; 2009;14(5):273–6.
IEEE
[1]
D. de Graaf, W. Ritter, F. Jacobs, and M. Brunain, “Lessons from the first international proficiency test for the detection of spores from the honey bee pathogen Paenibacillus larvae,” ACCREDITATION AND QUALITY ASSURANCE, vol. 14, no. 5, pp. 273–276, 2009.
@article{700025,
  abstract     = {{We report on the first proficiency test in the context of honey bee disease testing on a broad international scale. Honey samples were distributed to 12 participating laboratories for isolation and identification of spores of the etiological agent of American foulbrood, Paenibacillus larvae. Of the 11 laboratories responding to this proficiency testing event, only 6 (54%) provided data that were in full agreement with the previously determined status of the honey samples; eight (72%) laboratories fell within the threshold for qualification as defined a priori in this proficiency test (=level of agreement of at least 90%). Some lessons can now be drawn from the organizational point of view and will certainly improve new initiatives to be taken.}},
  author       = {{de Graaf, Dirk and Ritter, Wolfgang and Jacobs, Franciscus and Brunain, Marleen}},
  issn         = {{0949-1775}},
  journal      = {{ACCREDITATION AND QUALITY ASSURANCE}},
  keywords     = {{AMERICAN FOULBROOD DISEASE,GENOTYPES,VIRULENCE,PNEUMONIAE,DIAGNOSIS,RESISTANCE,LABORATORIES,SUBSP LARVAE,BACILLUS-LARVAE,APIS-MELLIFERA}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{273--276}},
  publisher    = {{Springer}},
  title        = {{Lessons from the first international proficiency test for the detection of spores from the honey bee pathogen Paenibacillus larvae}},
  url          = {{http://dx.doi.org/10.1007/s00769-009-0495-x}},
  volume       = {{14}},
  year         = {{2009}},
}

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