Advanced search
1 file | 422.98 KB

Enhancement of neoangiogenesis and follicle survival by sphingosine-1-phosphate in human ovarian tissue xenotransplants

(2011) PLOS ONE. 6(4).
Author
Organization
Abstract
Ovarian transplantation is one of the key approaches to restoring fertility in women who became menopausal as a result of cancer treatments. A major limitation of human ovarian transplants is massive follicular loss during revascularization. Here we investigated whether sphingosine-1-phosphate or its receptor agonists could enhance neoangiogenesis and follicle survival in ovarian transplants in a xenograft model. Human ovarian tissue xenografts in severe-combined-immunodeficient mice were treated with sphingosine-1-phosphate, its analogs, or vehicle for 1-10 days. We found that sphingosine-1-phosphate treatment increased vascular density in ovarian transplants significantly whereas FTY720 and SEW2871 had the opposite effect. In addition, sphingosine-1-phosphate accelerated the angiogenic process compared to vehicle-treated controls. Furthermore, sphingosine-1-phosphate treatment was associated with a significant proliferation of ovarian stromal cell as well as reduced necrosis and tissue hypoxia compared to the vehicle-treated controls. This resulted in a significantly lower percentage of apoptotic follicles in sphingosine-1-phosphate-treated transplants. We conclude that while sphingosine-1-phosphate promotes neoangiogenesis in ovarian transplants and reduces ischemic reperfusion injury, sphingosine-1-phosphate receptor agonists appear to functionally antagonize this process. Sphingosine-1-phosphate holds great promise to clinically enhance the survival and longevity of human autologous ovarian transplants.
Keywords
ENDOTHELIAL-CELLS, EXTRACELLULAR-MATRIX, RECEPTOR EDG-1, TRANSPLANTATION, FTY720, ANGIOGENESIS, ACTIVIN-A, LYSOPHOSPHATIDIC ACID, SPHINGOSINE 1-PHOSPHATE, ISCHEMIA-REPERFUSION INJURY

Downloads

  • Enhancement of Neoangiogenesis.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 422.98 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Soleimani, Reza, Elke Heytens, and Kutluk Oktay. 2011. “Enhancement of Neoangiogenesis and Follicle Survival by Sphingosine-1-phosphate in Human Ovarian Tissue Xenotransplants.” Plos One 6 (4).
APA
Soleimani, R., Heytens, E., & Oktay, K. (2011). Enhancement of neoangiogenesis and follicle survival by sphingosine-1-phosphate in human ovarian tissue xenotransplants. PLOS ONE, 6(4).
Vancouver
1.
Soleimani R, Heytens E, Oktay K. Enhancement of neoangiogenesis and follicle survival by sphingosine-1-phosphate in human ovarian tissue xenotransplants. PLOS ONE. 2011;6(4).
MLA
Soleimani, Reza, Elke Heytens, and Kutluk Oktay. “Enhancement of Neoangiogenesis and Follicle Survival by Sphingosine-1-phosphate in Human Ovarian Tissue Xenotransplants.” PLOS ONE 6.4 (2011): n. pag. Print.
@article{6999490,
  abstract     = {Ovarian transplantation is one of the key approaches to restoring fertility in women who became menopausal as a result of cancer treatments. A major limitation of human ovarian transplants is massive follicular loss during revascularization. Here we investigated whether sphingosine-1-phosphate or its receptor agonists could enhance neoangiogenesis and follicle survival in ovarian transplants in a xenograft model. Human ovarian tissue xenografts in severe-combined-immunodeficient mice were treated with sphingosine-1-phosphate, its analogs, or vehicle for 1-10 days. We found that sphingosine-1-phosphate treatment increased vascular density in ovarian transplants significantly whereas FTY720 and SEW2871 had the opposite effect. In addition, sphingosine-1-phosphate accelerated the angiogenic process compared to vehicle-treated controls. Furthermore, sphingosine-1-phosphate treatment was associated with a significant proliferation of ovarian stromal cell as well as reduced necrosis and tissue hypoxia compared to the vehicle-treated controls. This resulted in a significantly lower percentage of apoptotic follicles in sphingosine-1-phosphate-treated transplants. We conclude that while sphingosine-1-phosphate promotes neoangiogenesis in ovarian transplants and reduces ischemic reperfusion injury, sphingosine-1-phosphate receptor agonists appear to functionally antagonize this process. Sphingosine-1-phosphate holds great promise to clinically enhance the survival and longevity of human autologous ovarian transplants.},
  articleno    = {e19475},
  author       = {Soleimani, Reza and Heytens, Elke and Oktay, Kutluk},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {ENDOTHELIAL-CELLS,EXTRACELLULAR-MATRIX,RECEPTOR EDG-1,TRANSPLANTATION,FTY720,ANGIOGENESIS,ACTIVIN-A,LYSOPHOSPHATIDIC ACID,SPHINGOSINE 1-PHOSPHATE,ISCHEMIA-REPERFUSION INJURY},
  language     = {eng},
  number       = {4},
  pages        = {8},
  title        = {Enhancement of neoangiogenesis and follicle survival by sphingosine-1-phosphate in human ovarian tissue xenotransplants},
  url          = {http://dx.doi.org/10.1371/journal.pone.0019475},
  volume       = {6},
  year         = {2011},
}

Altmetric
View in Altmetric
Web of Science
Times cited: