X-ray fluorescence imaging of biological samples on the (sub)micron scale using specialized sample environments
(2015)
- Author
- Eva Vergucht (UGent)
- Promoter
- Laszlo Vincze (UGent) , Frank Vanhaecke (UGent) and Manfred Burghammer (UGent)
- Organization
- Abstract
- In the bioanalysis research field, single cell studies seek for deeper insights in biological processes through cellular analysis which require quantitative techniques with high elemental and chemical sensitivity combined with high spatial resolution, such as synchrotron radiation based X-ray fluorescence imaging. When performing X-ray micro-analysis of biological with the aim of obtaining results representative to their natural state as closely as possible, two distinct approaches can be followed, i.e. modifying the sample and making it suitable for analysis and/or optimizing the applied analytical methodology. This study aimed at the development and application of two specialized sample measurement environments for the analysis of biological organisms close to the in vivo state. The use of optical tweezers enabled truly free-standing investigation of microscopic samples, combined with performing non-contact sample manipulation and positioning. In this way two-dimensional elemental micro-/nano-analysis can be performed on essentially living biological samples, allowing to step away from the generally accepted trend of applying time-consuming, often invasive sample preparation steps prior to analysis. In addition, biological samples were investigated under cryogenic conditions using a state-of-the-art cryogenic in-vacuum sample environment, thus remaining close to the natural state and reducing the probability of radiation damage due to the ice matrix.
Downloads
-
phd vergucht suppl videos.rar
- full text
- |
- open access
- |
- application/x-rar
- |
- 40.76 MB
-
phd vergucht final.pdf
- full text
- |
- open access
- |
- |
- 105.14 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-6993435
- MLA
- Vergucht, Eva. X-Ray Fluorescence Imaging of Biological Samples on the (Sub)Micron Scale Using Specialized Sample Environments. Ghent University. Faculty of Sciences, 2015.
- APA
- Vergucht, E. (2015). X-ray fluorescence imaging of biological samples on the (sub)micron scale using specialized sample environments. Ghent University. Faculty of Sciences, Ghent, Belgium.
- Chicago author-date
- Vergucht, Eva. 2015. “X-Ray Fluorescence Imaging of Biological Samples on the (Sub)Micron Scale Using Specialized Sample Environments.” Ghent, Belgium: Ghent University. Faculty of Sciences.
- Chicago author-date (all authors)
- Vergucht, Eva. 2015. “X-Ray Fluorescence Imaging of Biological Samples on the (Sub)Micron Scale Using Specialized Sample Environments.” Ghent, Belgium: Ghent University. Faculty of Sciences.
- Vancouver
- 1.Vergucht E. X-ray fluorescence imaging of biological samples on the (sub)micron scale using specialized sample environments. [Ghent, Belgium]: Ghent University. Faculty of Sciences; 2015.
- IEEE
- [1]E. Vergucht, “X-ray fluorescence imaging of biological samples on the (sub)micron scale using specialized sample environments,” Ghent University. Faculty of Sciences, Ghent, Belgium, 2015.
@phdthesis{6993435, abstract = {{In the bioanalysis research field, single cell studies seek for deeper insights in biological processes through cellular analysis which require quantitative techniques with high elemental and chemical sensitivity combined with high spatial resolution, such as synchrotron radiation based X-ray fluorescence imaging. When performing X-ray micro-analysis of biological with the aim of obtaining results representative to their natural state as closely as possible, two distinct approaches can be followed, i.e. modifying the sample and making it suitable for analysis and/or optimizing the applied analytical methodology. This study aimed at the development and application of two specialized sample measurement environments for the analysis of biological organisms close to the in vivo state. The use of optical tweezers enabled truly free-standing investigation of microscopic samples, combined with performing non-contact sample manipulation and positioning. In this way two-dimensional elemental micro-/nano-analysis can be performed on essentially living biological samples, allowing to step away from the generally accepted trend of applying time-consuming, often invasive sample preparation steps prior to analysis. In addition, biological samples were investigated under cryogenic conditions using a state-of-the-art cryogenic in-vacuum sample environment, thus remaining close to the natural state and reducing the probability of radiation damage due to the ice matrix.}}, author = {{Vergucht, Eva}}, language = {{eng}}, pages = {{XIII, 230 + CD-ROM}}, publisher = {{Ghent University. Faculty of Sciences}}, school = {{Ghent University}}, title = {{X-ray fluorescence imaging of biological samples on the (sub)micron scale using specialized sample environments}}, year = {{2015}}, }