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Population structure and spatio-temporal transmission dynamics of Plasmodium vivax after radical cure treatment in a rural village of the Peruvian Amazon

Author
Organization
Abstract
Background: Despite the large burden of Plasmodium vivax, little is known about its transmission dynamics. This study explored the population structure and spatio-temporal dynamics of P. vivax recurrent infections after radical cure in a two-year cohort study carried out in a rural community of the Peruvian Amazon. Methods: A total of 37 P. vivax participants recruited in San Carlos community (Peru) between April and December 2008 were treated radically with chloroquine and primaquine and followed up monthly for two years with systematic blood sampling. All samples were screened for malaria parasites and subsequently all P. vivax infections genotyped using 15 microsatellites. Parasite population structure and dynamics were determined by computing different genetic indices and using spatio-temporal statistics. Results: After radical cure, 76% of the study participants experienced one or more recurrent P. vivax infections, most of them sub-patent and asymptomatic. The parasite population displayed limited genetic diversity (He = 0.49) and clonal structure, with most infections (84%) being monoclonal. Spatio-temporal clusters of specific haplotypes were found throughout the study and persistence of highly frequent haplotypes were observed over several months within the same participants/households. Conclusions: In San Carlos community, P. vivax recurrences were commonly observed after radical treatment, and characterized by asymptomatic, sub-patent and clustered infections (within and between individuals from a few neighbouring households). Moreover low genetic diversity as well as parasite inbreeding are likely to define a clonal parasite population which has important implications on the malaria epidemiology of the study area.
Keywords
Malaria, Plasmodium vivax, Genotyping, Microsatellites, Population genetics, Spatio-temporal analysis, Peruvian amazon, MICROSATELLITE MARKERS, MALARIA CONTROL, FALCIPARUM, INFECTION, ELIMINATION, RESISTANCE, REGION, POLYMORPHISM, CHLOROQUINE, COMPLEXITY

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MLA
Delgado-Ratto, Christopher et al. “Population Structure and Spatio-temporal Transmission Dynamics of Plasmodium Vivax After Radical Cure Treatment in a Rural Village of the Peruvian Amazon.” MALARIA JOURNAL 13 (2014): n. pag. Print.
APA
Delgado-Ratto, C., Soto-Calle, V. E., Van den Eede, P., Gamboa, D., Rosas, A., Abatih, E., Rodriguez Ferrucci, H., et al. (2014). Population structure and spatio-temporal transmission dynamics of Plasmodium vivax after radical cure treatment in a rural village of the Peruvian Amazon. MALARIA JOURNAL, 13.
Chicago author-date
Delgado-Ratto, Christopher, Veronica E Soto-Calle, Peter Van den Eede, Dionicia Gamboa, Angel Rosas, Emmanuel Abatih, Hugo Rodriguez Ferrucci, et al. 2014. “Population Structure and Spatio-temporal Transmission Dynamics of Plasmodium Vivax After Radical Cure Treatment in a Rural Village of the Peruvian Amazon.” Malaria Journal 13.
Chicago author-date (all authors)
Delgado-Ratto, Christopher, Veronica E Soto-Calle, Peter Van den Eede, Dionicia Gamboa, Angel Rosas, Emmanuel Abatih, Hugo Rodriguez Ferrucci, Alejandro Llanos-Cuentas, Jean-Pierre Van Geertruyden, Annette Erhart, and Umberto D’Alessandro. 2014. “Population Structure and Spatio-temporal Transmission Dynamics of Plasmodium Vivax After Radical Cure Treatment in a Rural Village of the Peruvian Amazon.” Malaria Journal 13.
Vancouver
1.
Delgado-Ratto C, Soto-Calle VE, Van den Eede P, Gamboa D, Rosas A, Abatih E, et al. Population structure and spatio-temporal transmission dynamics of Plasmodium vivax after radical cure treatment in a rural village of the Peruvian Amazon. MALARIA JOURNAL. 2014;13.
IEEE
[1]
C. Delgado-Ratto et al., “Population structure and spatio-temporal transmission dynamics of Plasmodium vivax after radical cure treatment in a rural village of the Peruvian Amazon,” MALARIA JOURNAL, vol. 13, 2014.
@article{6992418,
  abstract     = {Background: Despite the large burden of Plasmodium vivax, little is known about its transmission dynamics. This study explored the population structure and spatio-temporal dynamics of P. vivax recurrent infections after radical cure in a two-year cohort study carried out in a rural community of the Peruvian Amazon. 
Methods: A total of 37 P. vivax participants recruited in San Carlos community (Peru) between April and December 2008 were treated radically with chloroquine and primaquine and followed up monthly for two years with systematic blood sampling. All samples were screened for malaria parasites and subsequently all P. vivax infections genotyped using 15 microsatellites. Parasite population structure and dynamics were determined by computing different genetic indices and using spatio-temporal statistics. 
Results: After radical cure, 76% of the study participants experienced one or more recurrent P. vivax infections, most of them sub-patent and asymptomatic. The parasite population displayed limited genetic diversity (He = 0.49) and clonal structure, with most infections (84%) being monoclonal. Spatio-temporal clusters of specific haplotypes were found throughout the study and persistence of highly frequent haplotypes were observed over several months within the same participants/households. 
Conclusions: In San Carlos community, P. vivax recurrences were commonly observed after radical treatment, and characterized by asymptomatic, sub-patent and clustered infections (within and between individuals from a few neighbouring households). Moreover low genetic diversity as well as parasite inbreeding are likely to define a clonal parasite population which has important implications on the malaria epidemiology of the study area.},
  articleno    = {8},
  author       = {Delgado-Ratto, Christopher and Soto-Calle, Veronica E and Van den Eede, Peter and Gamboa, Dionicia and Rosas, Angel and Abatih, Emmanuel and Rodriguez Ferrucci, Hugo and Llanos-Cuentas, Alejandro and Van Geertruyden, Jean-Pierre and Erhart, Annette and D'Alessandro, Umberto},
  issn         = {1475-2875},
  journal      = {MALARIA JOURNAL},
  keywords     = {Malaria,Plasmodium vivax,Genotyping,Microsatellites,Population genetics,Spatio-temporal analysis,Peruvian amazon,MICROSATELLITE MARKERS,MALARIA CONTROL,FALCIPARUM,INFECTION,ELIMINATION,RESISTANCE,REGION,POLYMORPHISM,CHLOROQUINE,COMPLEXITY},
  language     = {eng},
  pages        = {12},
  title        = {Population structure and spatio-temporal transmission dynamics of Plasmodium vivax after radical cure treatment in a rural village of the Peruvian Amazon},
  url          = {http://dx.doi.org/10.1186/1475-2875-13-8},
  volume       = {13},
  year         = {2014},
}

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