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Studying Tissue-Specific Distributions of Metals in Daphnia magna using Scanning X-ray Fluorescence and Absorption Microtomography

(2007) p.317-317
Author
Organization
Abstract
Daphnia magna are being used to study the mechanism of intoxication by metals (Zn, Cu, Ni) and develop so called biotic ligand models (BLM). It has been shown that Daphnia's respond differently to Zn-exposure via the water and/or via the food. By means of tracer experiments with stable isotopes it is possible to evaluate the importance of different exposure routes using ICP-MS (inductively coupled plasma mass spectrometry). As a complementary approach to this problem, we explore the potential of combined X-ray micro fluorescence techniques: synchtrotron radiation micro-XRF ( micro-XRF dynamic scanning, micro XRF computed tomography and confocal micro XRF) in order to distinguish critical tissue accumulation from whole body accumulation. Moreover, the combination of these techniques with absorption microtomography reveals tissue specific distribution of metals in Daphnia. Furthermore, comparing measurements om differently exposed Daphnia samples allows us to gain insight in the tissue specific metabolism of heavy metals
Keywords
synchrotron, XRF, Daphnia magna, exotoxicology

Citation

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Chicago
De Samber, Björn, Geert Silversmit, Tom Schoonjans, Roel Evens, Karel De Schamphelaere, Colin Janssen, Bert Masschaele, et al. 2007. “Studying Tissue-Specific Distributions of Metals in Daphnia Magna Using Scanning X-ray Fluorescence and Absorption Microtomography.” In , 317–317.
APA
De Samber, B., Silversmit, G., Schoonjans, T., Evens, R., De Schamphelaere, K., Janssen, C., Masschaele, B., et al. (2007). Studying Tissue-Specific Distributions of Metals in Daphnia magna using Scanning X-ray Fluorescence and Absorption Microtomography (pp. 317–317). Presented at the EUROAnalysis XIV.
Vancouver
1.
De Samber B, Silversmit G, Schoonjans T, Evens R, De Schamphelaere K, Janssen C, et al. Studying Tissue-Specific Distributions of Metals in Daphnia magna using Scanning X-ray Fluorescence and Absorption Microtomography. 2007. p. 317–317.
MLA
De Samber, Björn, Geert Silversmit, Tom Schoonjans, et al. “Studying Tissue-Specific Distributions of Metals in Daphnia Magna Using Scanning X-ray Fluorescence and Absorption Microtomography.” 2007. 317–317. Print.
@inproceedings{699240,
  abstract     = {Daphnia magna are being used to study the mechanism of intoxication by metals (Zn, Cu, Ni) and develop so called biotic ligand models (BLM). It has been shown that Daphnia's respond differently to Zn-exposure via the water and/or via the food. By means of tracer experiments with stable isotopes it is possible to evaluate the importance of different exposure routes using ICP-MS (inductively coupled plasma mass spectrometry).

As a complementary approach to this problem, we explore the potential of combined X-ray micro fluorescence techniques: synchtrotron radiation micro-XRF ( micro-XRF dynamic scanning, micro XRF computed tomography and confocal micro XRF) in order to distinguish critical tissue accumulation from whole body accumulation. Moreover, the combination of these techniques with absorption microtomography reveals tissue specific distribution of metals in Daphnia. Furthermore, comparing measurements om differently exposed Daphnia samples allows us to gain insight in the tissue specific metabolism of heavy metals},
  author       = {De Samber, Bj{\"o}rn and Silversmit, Geert and Schoonjans, Tom and Evens, Roel and De Schamphelaere, Karel and Janssen, Colin and Masschaele, Bert and Van Hoorebeke, Luc and Balcaen, Lieve and Vanhaecke, Frank and Falkenberg, Gerald and Vincze, Laszlo},
  keyword      = {synchrotron,XRF,Daphnia magna,exotoxicology},
  language     = {eng},
  location     = {Antwerp, Belgium},
  pages        = {317--317},
  title        = {Studying Tissue-Specific Distributions of Metals in Daphnia magna using Scanning X-ray Fluorescence and Absorption Microtomography},
  year         = {2007},
}