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Studying Tissue-Specific Distributions of Metals in Daphnia magna using Scanning X-ray Fluorescence and Absorption Microtomography

Björn De Samber UGent, Geert Silversmit UGent, Tom Schoonjans UGent, Roel Evens UGent, Karel De Schamphelaere UGent, Colin Janssen UGent, Bert Masschaele UGent, Luc Van Hoorebeke UGent, Lieve Balcaen UGent and Frank Vanhaecke UGent, et al. (2007) p.317-317
abstract
Daphnia magna are being used to study the mechanism of intoxication by metals (Zn, Cu, Ni) and develop so called biotic ligand models (BLM). It has been shown that Daphnia's respond differently to Zn-exposure via the water and/or via the food. By means of tracer experiments with stable isotopes it is possible to evaluate the importance of different exposure routes using ICP-MS (inductively coupled plasma mass spectrometry). As a complementary approach to this problem, we explore the potential of combined X-ray micro fluorescence techniques: synchtrotron radiation micro-XRF ( micro-XRF dynamic scanning, micro XRF computed tomography and confocal micro XRF) in order to distinguish critical tissue accumulation from whole body accumulation. Moreover, the combination of these techniques with absorption microtomography reveals tissue specific distribution of metals in Daphnia. Furthermore, comparing measurements om differently exposed Daphnia samples allows us to gain insight in the tissue specific metabolism of heavy metals
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
synchrotron, XRF, Daphnia magna, exotoxicology
pages
1 pages
conference name
EUROAnalysis XIV
conference location
Antwerp, Belgium
conference start
2007-09-09
conference end
2009-09-14
language
English
UGent publication?
yes
classification
C3
id
699240
handle
http://hdl.handle.net/1854/LU-699240
date created
2009-06-15 12:39:54
date last changed
2009-06-29 11:24:02
@inproceedings{699240,
  abstract     = {Daphnia magna are being used to study the mechanism of intoxication by metals (Zn, Cu, Ni) and develop so called biotic ligand models (BLM). It has been shown that Daphnia's respond differently to Zn-exposure via the water and/or via the food. By means of tracer experiments with stable isotopes it is possible to evaluate the importance of different exposure routes using ICP-MS (inductively coupled plasma mass spectrometry).

As a complementary approach to this problem, we explore the potential of combined X-ray micro fluorescence techniques: synchtrotron radiation micro-XRF ( micro-XRF dynamic scanning, micro XRF computed tomography and confocal micro XRF) in order to distinguish critical tissue accumulation from whole body accumulation. Moreover, the combination of these techniques with absorption microtomography reveals tissue specific distribution of metals in Daphnia. Furthermore, comparing measurements om differently exposed Daphnia samples allows us to gain insight in the tissue specific metabolism of heavy metals},
  author       = {De Samber, Bj{\"o}rn and Silversmit, Geert and Schoonjans, Tom and Evens, Roel and De Schamphelaere, Karel and Janssen, Colin and Masschaele, Bert and Van Hoorebeke, Luc and Balcaen, Lieve and Vanhaecke, Frank and Falkenberg, Gerald and Vincze, Laszlo},
  keyword      = {synchrotron,XRF,Daphnia magna,exotoxicology},
  language     = {eng},
  location     = {Antwerp, Belgium},
  pages        = {317--317},
  title        = {Studying Tissue-Specific Distributions of Metals in Daphnia magna using Scanning X-ray Fluorescence and Absorption Microtomography},
  year         = {2007},
}

Chicago
De Samber, Björn, Geert Silversmit, Tom Schoonjans, Roel Evens, Karel De Schamphelaere, Colin Janssen, Bert Masschaele, et al. 2007. “Studying Tissue-Specific Distributions of Metals in Daphnia Magna Using Scanning X-ray Fluorescence and Absorption Microtomography.” In , 317–317.
APA
De Samber, B., Silversmit, G., Schoonjans, T., Evens, R., De Schamphelaere, K., Janssen, C., Masschaele, B., et al. (2007). Studying Tissue-Specific Distributions of Metals in Daphnia magna using Scanning X-ray Fluorescence and Absorption Microtomography (pp. 317–317). Presented at the EUROAnalysis XIV.
Vancouver
1.
De Samber B, Silversmit G, Schoonjans T, Evens R, De Schamphelaere K, Janssen C, et al. Studying Tissue-Specific Distributions of Metals in Daphnia magna using Scanning X-ray Fluorescence and Absorption Microtomography. 2007. p. 317–317.
MLA
De Samber, Björn, Geert Silversmit, Tom Schoonjans, et al. “Studying Tissue-Specific Distributions of Metals in Daphnia Magna Using Scanning X-ray Fluorescence and Absorption Microtomography.” 2007. 317–317. Print.