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Quantifying the contribution of entire free-living nematode communities to carbon mineralization under contrasting C and N availability

Mesfin Tsegaye Gebremikael UGent, Hanne Steel UGent, Wim Bert UGent, Peter Maenhout UGent, Steven Sleutel UGent and Stefaan De Neve UGent (2015) PLOS ONE. 10(9).
abstract
To understand the roles of nematodes in organic matter (OM) decomposition, experimental setups should include the entire nematode community, the native soil microflora, and their food sources. Yet, published studies are often based on either simplified experimental setups, using only a few selected species of nematode and their respective prey, despite the multitude of species present in natural soil, or on indirect estimation of the mineralization process using O-2 consumption and the fresh weight of nematodes. We set up a six-month incubation experiment to quantify the contribution of the entire free living nematode community to carbon (C) mineralization under realistic conditions. The following treatments were compared with and without grass-clover amendment: defaunated soil reinoculated with the entire free living nematode communities (+Nem) and defaunated soil that was not reinoculated (-Nem). We also included untreated fresh soil as a control (CTR). Nematode abundances and diversity in +Nem was comparable to the CTR showing the success of the reinoculation. No significant differences in C mineralization were found between + Nem and -Nem treatments of the amended and unamended samples at the end of incubation. Other related parameters such as microbial biomass C and enzymatic activities did not show significant differences between + Nem and -Nem treatments in both amended and unamended samples. These findings show that the collective contribution of the entire nematode community to C mineralization is small. Previous reports in literature based on simplified experimental setups and indirect estimations are contrasting with the findings of the current study and further investigations are needed to elucidate the extent and the mechanisms of nematode involvement in C mineralization.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NITROGEN MINERALIZATION, SOIL MICROBIAL COMMUNITIES, TERRESTRIAL ECOSYSTEMS, POPULATION ENERGETICS, DYNAMICS, BIOMASS, PROTOZOA, ENERGY, DECOMPOSITION, BACTERIAL-FEEDING NEMATODES
journal title
PLOS ONE
PLoS One
volume
10
issue
9
article number
e0136244
pages
17 pages
Web of Science type
Article
Web of Science id
000361792100007
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.057 (2015)
JCR rank
11/63 (2015)
JCR quartile
1 (2015)
ISSN
1932-6203
DOI
10.1371/journal.pone.0136244
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
6988367
handle
http://hdl.handle.net/1854/LU-6988367
date created
2015-11-23 10:45:51
date last changed
2016-12-21 15:41:48
@article{6988367,
  abstract     = {To understand the roles of nematodes in organic matter (OM) decomposition, experimental setups should include the entire nematode community, the native soil microflora, and their food sources. Yet, published studies are often based on either simplified experimental setups, using only a few selected species of nematode and their respective prey, despite the multitude of species present in natural soil, or on indirect estimation of the mineralization process using O-2 consumption and the fresh weight of nematodes. We set up a six-month incubation experiment to quantify the contribution of the entire free living nematode community to carbon (C) mineralization under realistic conditions. The following treatments were compared with and without grass-clover amendment: defaunated soil reinoculated with the entire free living nematode communities (+Nem) and defaunated soil that was not reinoculated (-Nem). We also included untreated fresh soil as a control (CTR). Nematode abundances and diversity in +Nem was comparable to the CTR showing the success of the reinoculation. No significant differences in C mineralization were found between + Nem and -Nem treatments of the amended and unamended samples at the end of incubation. Other related parameters such as microbial biomass C and enzymatic activities did not show significant differences between + Nem and -Nem treatments in both amended and unamended samples. These findings show that the collective contribution of the entire nematode community to C mineralization is small. Previous reports in literature based on simplified experimental setups and indirect estimations are contrasting with the findings of the current study and further investigations are needed to elucidate the extent and the mechanisms of nematode involvement in C mineralization.},
  articleno    = {e0136244},
  author       = {Gebremikael, Mesfin Tsegaye and Steel, Hanne and Bert, Wim and Maenhout, Peter and Sleutel, Steven and De Neve, Stefaan},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {NITROGEN MINERALIZATION,SOIL MICROBIAL COMMUNITIES,TERRESTRIAL ECOSYSTEMS,POPULATION ENERGETICS,DYNAMICS,BIOMASS,PROTOZOA,ENERGY,DECOMPOSITION,BACTERIAL-FEEDING NEMATODES},
  language     = {eng},
  number       = {9},
  pages        = {17},
  title        = {Quantifying the contribution of entire free-living nematode communities to carbon mineralization under contrasting C and N availability},
  url          = {http://dx.doi.org/10.1371/journal.pone.0136244},
  volume       = {10},
  year         = {2015},
}

Chicago
Gebremikael, Mesfin Tsegaye, Hanne Steel, Wim Bert, Peter Maenhout, Steven Sleutel, and Stefaan De Neve. 2015. “Quantifying the Contribution of Entire Free-living Nematode Communities to Carbon Mineralization Under Contrasting C and N Availability.” Plos One 10 (9).
APA
Gebremikael, M. T., Steel, H., Bert, W., Maenhout, P., Sleutel, S., & De Neve, S. (2015). Quantifying the contribution of entire free-living nematode communities to carbon mineralization under contrasting C and N availability. PLOS ONE, 10(9).
Vancouver
1.
Gebremikael MT, Steel H, Bert W, Maenhout P, Sleutel S, De Neve S. Quantifying the contribution of entire free-living nematode communities to carbon mineralization under contrasting C and N availability. PLOS ONE. 2015;10(9).
MLA
Gebremikael, Mesfin Tsegaye, Hanne Steel, Wim Bert, et al. “Quantifying the Contribution of Entire Free-living Nematode Communities to Carbon Mineralization Under Contrasting C and N Availability.” PLOS ONE 10.9 (2015): n. pag. Print.