Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
Add to list
  1. Search results
  2. Three dimensional SR-confocal micro X...

Three dimensional SR-confocal micro XRF combined with absorption microtomography on Daphnia magna: Instrumental developments and recent advances in dataprocessing

Björn De Samber (UGent) , Roel Evens (UGent) , Tom Schoonjans (UGent) , Geert Silversmit (UGent) , Bart Vekemans (UGent) , Karel De Schamphelaere (UGent) , Colin Janssen (UGent) , Bert Masschaele (UGent) , Luc Van Hoorebeke (UGent) , Karen Rickers, et al.
(2008) Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry. p.54-54
Author
Organization
Abstract
Daphnia magna are being used to study the mechanism of intoxication by metals (Zn, Cu, Ni) and to develop so called biotic ligand models (BLM's). It has been shown that Daphnia magna responds differently to Zn-exposure via the water and/or via the food. In order to distinguish critical tissue from whole body accumulation in these small organisms (3mm size), we explored the potential of combined X-ray techniques as a complementary approach to the above problem. Fast dynamic 2D micro XRF scanning and XRF micro CT experiments on Daphnia magna at Beamline L (HASYLAB, Hamburg, Germany) allowed the semi-quantitative investigation of the accumulation of metals within specific organs with microscopic resolution. Combining these results with micro CT data sets unravelled the tissue specifi 2D/3D distribution of metals within delicate organic sample on the 3-15 mu resolution revel in an essentially non-destructive manner. As demonstrated, recent developments at beamline L, involving a dedicated confocal micro-XRF set-up with a resolution of 5 mu and full 3D dynamic scanning algorithms allowed to explore microscopic 3D substructures within Daphnia magna (also called "top-down" methodology) aiding ecotoxicological research towards the tissue and even cellular level. Three dimensional isosurfaces of the elements of interest were obtained within a 300 mu Daphnia magna egg. Moreover, K-means clustering allowed us to isolate 3D clusters of interest of which the elemental content can be investigated in closer detail. Finally, using laboratory micro-CT, we obtained full 3D correlation between elemental and absorption datasets on the micrometer scale within biological samples.
Keywords
confocal micro-XRF

Citation

Please use this url to cite or link to this publication:

MLA
De Samber, Björn, et al. “Three Dimensional SR-Confocal Micro XRF Combined with Absorption Microtomography on Daphnia Magna: Instrumental Developments and Recent Advances in Dataprocessing.” Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry, edited by Stjepko Fazinić and Milko Jakšić, Ruđer Bošković Institute, 2008, pp. 54–54.
APA
De Samber, B., Evens, R., Schoonjans, T., Silversmit, G., Vekemans, B., De Schamphelaere, K., … Vincze, L. (2008). Three dimensional SR-confocal micro XRF combined with absorption microtomography on Daphnia magna: Instrumental developments and recent advances in dataprocessing. In S. Fazinić & M. Jakšić (Eds.), Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry (pp. 54–54). Zagreb, Croatia: Ruđer Bošković Institute.
Chicago author-date
De Samber, Björn, Roel Evens, Tom Schoonjans, Geert Silversmit, Bart Vekemans, Karel De Schamphelaere, Colin Janssen, et al. 2008. “Three Dimensional SR-Confocal Micro XRF Combined with Absorption Microtomography on Daphnia Magna: Instrumental Developments and Recent Advances in Dataprocessing.” In Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry, edited by Stjepko Fazinić and Milko Jakšić, 54–54. Zagreb, Croatia: Ruđer Bošković Institute.
Chicago author-date (all authors)
De Samber, Björn, Roel Evens, Tom Schoonjans, Geert Silversmit, Bart Vekemans, Karel De Schamphelaere, Colin Janssen, Bert Masschaele, Luc Van Hoorebeke, Karen Rickers, Gerald Falkenberg, and Laszlo Vincze. 2008. “Three Dimensional SR-Confocal Micro XRF Combined with Absorption Microtomography on Daphnia Magna: Instrumental Developments and Recent Advances in Dataprocessing.” In Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry, ed by. Stjepko Fazinić and Milko Jakšić, 54–54. Zagreb, Croatia: Ruđer Bošković Institute.
Vancouver
1.
De Samber B, Evens R, Schoonjans T, Silversmit G, Vekemans B, De Schamphelaere K, et al. Three dimensional SR-confocal micro XRF combined with absorption microtomography on Daphnia magna: Instrumental developments and recent advances in dataprocessing. In: Fazinić S, Jakšić M, editors. Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry. Zagreb, Croatia: Ruđer Bošković Institute; 2008. p. 54–54.
IEEE
[1]
B. De Samber et al., “Three dimensional SR-confocal micro XRF combined with absorption microtomography on Daphnia magna: Instrumental developments and recent advances in dataprocessing,” in Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry, Cavtat, Dubrovnik, Croatia, 2008, pp. 54–54.
@inproceedings{698791,
  abstract     = {{Daphnia magna are being used to study the mechanism of intoxication by metals (Zn, Cu, Ni) and to develop so called biotic ligand models (BLM's). It has been shown that Daphnia magna responds differently to Zn-exposure via the water and/or via the food.
In order to distinguish critical tissue from whole body accumulation in these small organisms (3mm size), we explored the potential of combined X-ray techniques as a complementary approach to the above problem. Fast dynamic 2D micro XRF scanning and XRF micro CT experiments on Daphnia magna at Beamline L (HASYLAB, Hamburg, Germany) allowed the semi-quantitative investigation of the accumulation of metals within specific organs with microscopic resolution. Combining these results with micro CT data sets unravelled the tissue specifi 2D/3D distribution of metals within delicate organic sample on the 3-15 mu resolution revel in an essentially non-destructive manner.
As demonstrated, recent developments at beamline L, involving a dedicated confocal micro-XRF set-up with a resolution of 5 mu and full 3D dynamic scanning algorithms allowed to explore microscopic 3D substructures within Daphnia magna (also called "top-down" methodology) aiding ecotoxicological research towards the tissue and even cellular level. Three dimensional isosurfaces of the elements of interest were obtained within a 300 mu Daphnia magna egg. Moreover, K-means clustering allowed us to isolate 3D clusters of interest of which the elemental content can be investigated in closer detail. Finally, using laboratory micro-CT, we obtained full 3D correlation between elemental and absorption datasets on the micrometer scale within biological samples.}},
  author       = {{De Samber, Björn and Evens, Roel and Schoonjans, Tom and Silversmit, Geert and Vekemans, Bart and De Schamphelaere, Karel and Janssen, Colin and Masschaele, Bert and Van Hoorebeke, Luc and Rickers, Karen and Falkenberg, Gerald and Vincze, Laszlo}},
  booktitle    = {{Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry}},
  editor       = {{Fazinić, Stjepko and Jakšić, Milko}},
  isbn         = {{9789536690732}},
  keywords     = {{confocal micro-XRF}},
  language     = {{eng}},
  location     = {{Cavtat, Dubrovnik, Croatia}},
  pages        = {{54--54}},
  publisher    = {{Ruđer Bošković Institute}},
  title        = {{Three dimensional SR-confocal micro XRF combined with absorption microtomography on Daphnia magna: Instrumental developments and recent advances in dataprocessing}},
  url          = {{http://exrs2008.irb.hr/EXRS-BOA.pdf}},
  year         = {{2008}},
}