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Three dimensional SR-confocal micro XRF combined with absorption microtomography on Daphnia magna: Instrumental developments and recent advances in dataprocessing

Björn De Samber UGent, Roel Evens UGent, Tom Schoonjans UGent, Geert Silversmit UGent, Bart Vekemans UGent, Karel De Schamphelaere UGent, Colin Janssen UGent, Bert Masschaele UGent, Luc Van Hoorebeke UGent and Karen Rickers, et al. (2008) Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry. p.54-54
abstract
Daphnia magna are being used to study the mechanism of intoxication by metals (Zn, Cu, Ni) and to develop so called biotic ligand models (BLM's). It has been shown that Daphnia magna responds differently to Zn-exposure via the water and/or via the food. In order to distinguish critical tissue from whole body accumulation in these small organisms (3mm size), we explored the potential of combined X-ray techniques as a complementary approach to the above problem. Fast dynamic 2D micro XRF scanning and XRF micro CT experiments on Daphnia magna at Beamline L (HASYLAB, Hamburg, Germany) allowed the semi-quantitative investigation of the accumulation of metals within specific organs with microscopic resolution. Combining these results with micro CT data sets unravelled the tissue specifi 2D/3D distribution of metals within delicate organic sample on the 3-15 mu resolution revel in an essentially non-destructive manner. As demonstrated, recent developments at beamline L, involving a dedicated confocal micro-XRF set-up with a resolution of 5 mu and full 3D dynamic scanning algorithms allowed to explore microscopic 3D substructures within Daphnia magna (also called "top-down" methodology) aiding ecotoxicological research towards the tissue and even cellular level. Three dimensional isosurfaces of the elements of interest were obtained within a 300 mu Daphnia magna egg. Moreover, K-means clustering allowed us to isolate 3D clusters of interest of which the elemental content can be investigated in closer detail. Finally, using laboratory micro-CT, we obtained full 3D correlation between elemental and absorption datasets on the micrometer scale within biological samples.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
confocal micro-XRF
in
Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry
editor
Stjepko Fazinić and Milko Jakšić
pages
54 - 54
publisher
Ruđer Bošković Institute
place of publication
Zagreb, Croatia
conference name
13th European conference on X-Ray Spectrometry (EXRS 2008)
conference location
Cavtat, Dubrovnik, Croatia
conference start
2008-06-16
conference end
2008-08-20
ISBN
9789536690732
language
English
UGent publication?
yes
classification
C3
additional info
abstract no. O6-2
id
698791
handle
http://hdl.handle.net/1854/LU-698791
alternative location
http://exrs2008.irb.hr/EXRS-BOA.pdf
date created
2009-06-15 11:02:03
date last changed
2010-07-06 12:08:00
@inproceedings{698791,
  abstract     = {Daphnia magna are being used to study the mechanism of intoxication by metals (Zn, Cu, Ni) and to develop so called biotic ligand models (BLM's). It has been shown that Daphnia magna responds differently to Zn-exposure via the water and/or via the food.
In order to distinguish critical tissue from whole body accumulation in these small organisms (3mm size), we explored the potential of combined X-ray techniques as a complementary approach to the above problem. Fast dynamic 2D micro XRF scanning and XRF micro CT experiments on Daphnia magna at Beamline L (HASYLAB, Hamburg, Germany) allowed the semi-quantitative investigation of the accumulation of metals within specific organs with microscopic resolution. Combining these results with micro CT data sets unravelled the tissue specifi 2D/3D distribution of metals within delicate organic sample on the 3-15 mu resolution revel in an essentially non-destructive manner.
As demonstrated, recent developments at beamline L, involving a dedicated confocal micro-XRF set-up with a resolution of 5 mu and full 3D dynamic scanning algorithms allowed to explore microscopic 3D substructures within Daphnia magna (also called {\textacutedbl}top-down{\textacutedbl} methodology) aiding ecotoxicological research towards the tissue and even cellular level. Three dimensional isosurfaces of the elements of interest were obtained within a 300 mu Daphnia magna egg. Moreover, K-means clustering allowed us to isolate 3D clusters of interest of which the elemental content can be investigated in closer detail. Finally, using laboratory micro-CT, we obtained full 3D correlation between elemental and absorption datasets on the micrometer scale within biological samples.},
  author       = {De Samber, Bj{\"o}rn and Evens, Roel and Schoonjans, Tom and Silversmit, Geert and Vekemans, Bart and De Schamphelaere, Karel and Janssen, Colin and Masschaele, Bert and Van Hoorebeke, Luc and Rickers, Karen and Falkenberg, Gerald and Vincze, Laszlo},
  booktitle    = {Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry},
  editor       = {Fazini\'{c}, Stjepko and Jak\v{s}i\'{c}, Milko},
  isbn         = {9789536690732},
  keyword      = {confocal micro-XRF},
  language     = {eng},
  location     = {Cavtat, Dubrovnik, Croatia},
  pages        = {54--54},
  publisher    = {Ru\unmatched{0111}er Bo\v{s}kovi\'{c} Institute},
  title        = {Three dimensional SR-confocal micro XRF combined with absorption microtomography on Daphnia magna: Instrumental developments and recent advances in dataprocessing},
  url          = {http://exrs2008.irb.hr/EXRS-BOA.pdf},
  year         = {2008},
}

Chicago
De Samber, Björn, Roel Evens, Tom Schoonjans, Geert Silversmit, Bart Vekemans, Karel De Schamphelaere, Colin Janssen, et al. 2008. “Three Dimensional SR-confocal Micro XRF Combined with Absorption Microtomography on Daphnia Magna: Instrumental Developments and Recent Advances in Dataprocessing.” In Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry, ed. Stjepko Fazinić and Milko Jakšić, 54–54. Zagreb, Croatia: Ruđer Bošković Institute.
APA
De Samber, B., Evens, R., Schoonjans, T., Silversmit, G., Vekemans, B., De Schamphelaere, K., Janssen, C., et al. (2008). Three dimensional SR-confocal micro XRF combined with absorption microtomography on Daphnia magna: Instrumental developments and recent advances in dataprocessing. In S. Fazinić & M. Jakšić (Eds.), Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry (pp. 54–54). Presented at the 13th European conference on X-Ray Spectrometry (EXRS 2008), Zagreb, Croatia: Ruđer Bošković Institute.
Vancouver
1.
De Samber B, Evens R, Schoonjans T, Silversmit G, Vekemans B, De Schamphelaere K, et al. Three dimensional SR-confocal micro XRF combined with absorption microtomography on Daphnia magna: Instrumental developments and recent advances in dataprocessing. In: Fazinić S, Jakšić M, editors. Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry. Zagreb, Croatia: Ruđer Bošković Institute; 2008. p. 54–54.
MLA
De Samber, Björn, Roel Evens, Tom Schoonjans, et al. “Three Dimensional SR-confocal Micro XRF Combined with Absorption Microtomography on Daphnia Magna: Instrumental Developments and Recent Advances in Dataprocessing.” Book of Abstracts : EXRS-2008, European Conference on X-Ray Spectrometry. Ed. Stjepko Fazinić & Milko Jakšić. Zagreb, Croatia: Ruđer Bošković Institute, 2008. 54–54. Print.