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The effect of neuropeptide FF in the amygdala kindling model

(2016) ACTA NEUROLOGICA SCANDINAVICA. 134(3). p.181-188
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Abstract
Objective: Neuropeptide FF (NPFF) and its receptors (NPFF1R and NPFF2R) are differentially distributed throughout the central nervous system. NPFF reduces cortical excitability in rats when administered intracerebroventricularly (i.c.v.), and both NPFF and NPFF1R antagonists attenuate pilocarpine-induced limbic seizures. In this study, our aim was to determine whether NPFF exerts anticonvulsant or anti-epileptogenic effects in the rat amygdala kindling model for temporal lobe seizures. Methods: Male Wistar rats were implanted with a recording/stimulation electrode in the right amygdala and a cannula in the left lateral ventricle. In a first group of animals, the afterdischarge threshold (ADT) was determined after a single i.c.v. infusion of saline (n = 8) or NPFF (1 nmol/h for 2 h; n = 10). Subsequently, daily infusion of saline (n = 8) or NPFF (1 nmol/h for 2 h; i.c.v.; n = 9) was performed, followed by a kindling stimulus (ADT+200 A). Afterdischarge duration and seizure severity were evaluated after every kindling stimulus. A second group of rats (n = 7) were fully kindled, and the effect of saline or a high dose of NPFF (10 nmol/h for 2 h, i.c.v.) on ADT and the generalized seizure threshold (GST) was subsequently determined. Results: In naive rats, NPFF significantly increased the ADT compared to control (435 72 A vs 131 +/- 23 A [P < 0.05]). When rats underwent daily stimulations above the ADT, NPFF did not delay or prevent kindling acquisition. Furthermore, a high dose of NPFF did not alter ADT or GST in fully kindled rats. Conclusions: I.c.v. administration of NPFF reduced excitability in the amygdala in naive, but not in fully kindled rats, and had no effect on kindling acquisition.
Keywords
afterdischarge threshold, amygdala kindling model, generalized seizure threshold, kindling acquisition, neuropeptide FF, TEMPORAL-LOBE EPILEPSY, REDUCES FOOD-INTAKE, RAT HIPPOCAMPUS, ELECTRICAL-STIMULATION, STATUS EPILEPTICUS, NPFF RECEPTORS, ANIMAL-MODELS, IN-VIVO, ANTICONVULSANT, ANALGESIA

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Chicago
Buffel, Ine, Alfred Meurs, Jeanelle Portelli, Robrecht Raedt, Veerle De Herdt, Lindsay Poppe, V De Meulenaere, et al. 2016. “The Effect of Neuropeptide FF in the Amygdala Kindling Model.” Acta Neurologica Scandinavica 134 (3): 181–188.
APA
Buffel, I., Meurs, A., Portelli, J., Raedt, R., De Herdt, V., Poppe, L., De Meulenaere, V., et al. (2016). The effect of neuropeptide FF in the amygdala kindling model. ACTA NEUROLOGICA SCANDINAVICA, 134(3), 181–188.
Vancouver
1.
Buffel I, Meurs A, Portelli J, Raedt R, De Herdt V, Poppe L, et al. The effect of neuropeptide FF in the amygdala kindling model. ACTA NEUROLOGICA SCANDINAVICA. 2016;134(3):181–8.
MLA
Buffel, Ine, Alfred Meurs, Jeanelle Portelli, et al. “The Effect of Neuropeptide FF in the Amygdala Kindling Model.” ACTA NEUROLOGICA SCANDINAVICA 134.3 (2016): 181–188. Print.
@article{6986654,
  abstract     = {Objective: Neuropeptide FF (NPFF) and its receptors (NPFF1R and NPFF2R) are differentially distributed throughout the central nervous system. NPFF reduces cortical excitability in rats when administered intracerebroventricularly (i.c.v.), and both NPFF and NPFF1R antagonists attenuate pilocarpine-induced limbic seizures. In this study, our aim was to determine whether NPFF exerts anticonvulsant or anti-epileptogenic effects in the rat amygdala kindling model for temporal lobe seizures. 
Methods: Male Wistar rats were implanted with a recording/stimulation electrode in the right amygdala and a cannula in the left lateral ventricle. In a first group of animals, the afterdischarge threshold (ADT) was determined after a single i.c.v. infusion of saline (n = 8) or NPFF (1 nmol/h for 2 h; n = 10). Subsequently, daily infusion of saline (n = 8) or NPFF (1 nmol/h for 2 h; i.c.v.; n = 9) was performed, followed by a kindling stimulus (ADT+200 A). Afterdischarge duration and seizure severity were evaluated after every kindling stimulus. A second group of rats (n = 7) were fully kindled, and the effect of saline or a high dose of NPFF (10 nmol/h for 2 h, i.c.v.) on ADT and the generalized seizure threshold (GST) was subsequently determined. 
Results: In naive rats, NPFF significantly increased the ADT compared to control (435 72 A vs 131 +/- 23 A [P {\textlangle} 0.05]). When rats underwent daily stimulations above the ADT, NPFF did not delay or prevent kindling acquisition. Furthermore, a high dose of NPFF did not alter ADT or GST in fully kindled rats. 
Conclusions: I.c.v. administration of NPFF reduced excitability in the amygdala in naive, but not in fully kindled rats, and had no effect on kindling acquisition.},
  author       = {Buffel, Ine and Meurs, Alfred and Portelli, Jeanelle and Raedt, Robrecht and De Herdt, Veerle and Poppe, Lindsay and De Meulenaere, V and Wadman, Wytse and Bihel, F and Schmitt, M and Vonck, Kristl and  Bourguignon, J-J and Simonin, F and Smolders, I and Boon, Paul},
  issn         = {0001-6314},
  journal      = {ACTA NEUROLOGICA SCANDINAVICA},
  keyword      = {afterdischarge threshold,amygdala kindling model,generalized seizure threshold,kindling acquisition,neuropeptide FF,TEMPORAL-LOBE EPILEPSY,REDUCES FOOD-INTAKE,RAT HIPPOCAMPUS,ELECTRICAL-STIMULATION,STATUS EPILEPTICUS,NPFF RECEPTORS,ANIMAL-MODELS,IN-VIVO,ANTICONVULSANT,ANALGESIA},
  language     = {eng},
  number       = {3},
  pages        = {181--188},
  title        = {The effect of neuropeptide FF in the amygdala kindling model},
  url          = {http://dx.doi.org/10.1111/ane.12526},
  volume       = {134},
  year         = {2016},
}

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