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Laboratory and Synchrotron Radiation Micro and Nano X-ray Fluorescence

Björn De Samber UGent, Roel Evens UGent, Karel De Schamphelaere UGent, Bert Masschaele UGent, Geert Silversmit UGent, Tom Schoonjans UGent, Bart Vekemans UGent, Luc Van Hoorebeke UGent, Frank Vanhaecke UGent and Colin Janssen UGent, et al. (2009) p.45-46
abstract
Micro X-ray Fluorescence (µ-XRF) is a rapidly evolving analytical technique which allows visualizing the trace level metal distributions within a specimen in an essentially non-destructive manner. Using a laboratory µ-XRF spectrometer, ppm detection limits can be obtained with a spatial resolution of 10-20 µm. However, at second- and third generation synchrotron radiation (SR) sources, detection limits at the sub-ppm level can be obtained with a potential lateral resolution level better than 100 nm. These characteristics of micro/nanobeam SR-XRF allow spatially resolved multi-element determination of major, minor and trace constituents in microscopic sub-areas and volumes within biological specimens in an essentially non-destructive/non-invasive manner. However, the complexity of performing such an experiment is often quite considerable, involving dedicated sample preparation, transportation towards and experimenting at the synchrotron facility, installing an appropriate experimental set-up and performing a thorough data analysis on large amounts of spectral data. The ecotoxicological research on Daphnia magna, a frequently used model organism for investigating the mechanisms of toxicity of metals, has often been difficult because many analytical techniques are not able to investigate trace metal distributions in a spatially resolved manner at a (sub)microscopic resolution. A laboratory µ-XRF spectrometer (EDAX Eagle III) allowed us to precharacterize the major/minor element distributions within Daphnia magna with a moderate spatial resolution of approximately 20 µm. However, synchrotron radiation µ-XRF experiments were necessary (Beamline L, HASYLAB) with substantially increased elemental sensitivities to “virtually dissect” the tissue specific Zn accumulation within Daphnia magna. This work demonstrates the use of combined X-ray techniques, including two-dimensional (2D) µ-XRF, XRF micro-CT, confocal µ-XRF and absorption microtomography under conventional and cryogenic sample environments.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
ecotoxicology, synchrotron, Daphnia magna, micro-XRF
editor
Kouichi Tsuji
pages
2 pages
publisher
Sowa Information Control Center
place of publication
2-2-7, Higashi-Tenma, Kita-ku, Osaka, Japan
conference name
JST Symposium on "Micro and Trace X-ray Analysis"
conference location
Media Center, Osaka City University
conference start
2009-02-12
conference end
2009-02-14
language
English
UGent publication?
yes
classification
C3
copyright statement
I don't know the status of the copyright for this publication
id
698551
handle
http://hdl.handle.net/1854/LU-698551
date created
2009-06-15 10:35:33
date last changed
2009-06-26 16:08:21
@inproceedings{698551,
  abstract     = {Micro X-ray Fluorescence ({\textmu}-XRF) is a rapidly evolving analytical technique which allows visualizing the trace level metal distributions within a specimen in an essentially non-destructive manner. Using a laboratory {\textmu}-XRF spectrometer, ppm detection limits can be obtained with a spatial resolution of 10-20 {\textmu}m. However, at second- and third generation synchrotron radiation (SR) sources, detection limits at the sub-ppm level can be obtained with a potential lateral resolution level better than 100 nm. 

These characteristics of micro/nanobeam SR-XRF allow spatially resolved multi-element determination of major, minor and trace constituents in microscopic sub-areas and volumes within biological specimens in an essentially non-destructive/non-invasive manner. However, the complexity of performing such an experiment is often quite considerable, involving dedicated sample preparation, transportation towards and experimenting at the synchrotron facility, installing an appropriate experimental set-up and performing a thorough data analysis on large amounts of spectral data.

The ecotoxicological research on Daphnia magna, a frequently used model organism for investigating the mechanisms of toxicity of metals, has often been difficult because many analytical techniques are not able to investigate trace metal distributions in a spatially resolved manner at a (sub)microscopic resolution. A laboratory {\textmu}-XRF spectrometer (EDAX Eagle III) allowed us to precharacterize the major/minor element distributions within Daphnia magna with a moderate spatial resolution of approximately 20 {\textmu}m. However, synchrotron radiation {\textmu}-XRF experiments were necessary (Beamline L, HASYLAB) with substantially increased elemental sensitivities to {\textquotedblleft}virtually dissect{\textquotedblright} the tissue specific Zn accumulation within Daphnia magna. This work demonstrates the use of combined X-ray techniques, including two-dimensional (2D) {\textmu}-XRF, XRF micro-CT, confocal {\textmu}-XRF and absorption microtomography under conventional and cryogenic sample environments.},
  author       = {De Samber, Bj{\"o}rn and Evens, Roel and De Schamphelaere, Karel and Masschaele, Bert and Silversmit, Geert and Schoonjans, Tom and Vekemans, Bart and Van Hoorebeke, Luc and Vanhaecke, Frank and Janssen, Colin and Bohic, Sylvain and Wellenreuther, Gerd and Rickers, Karen and Falkenberg, Gerald and Vincze, Laszlo},
  editor       = {Tsuji, Kouichi},
  keyword      = {ecotoxicology,synchrotron,Daphnia magna,micro-XRF},
  language     = {eng},
  location     = {Media Center, Osaka City University},
  pages        = {45--46},
  publisher    = {Sowa Information Control Center},
  title        = {Laboratory and Synchrotron Radiation Micro and Nano X-ray Fluorescence},
  year         = {2009},
}

Chicago
De Samber, Björn, Roel Evens, Karel De Schamphelaere, Bert Masschaele, Geert Silversmit, Tom Schoonjans, Bart Vekemans, et al. 2009. “Laboratory and Synchrotron Radiation Micro and Nano X-ray Fluorescence.” In , ed. Kouichi Tsuji, 45–46. 2-2-7, Higashi-Tenma, Kita-ku, Osaka, Japan: Sowa Information Control Center.
APA
De Samber, B., Evens, R., De Schamphelaere, K., Masschaele, B., Silversmit, G., Schoonjans, T., Vekemans, B., et al. (2009). Laboratory and Synchrotron Radiation Micro and Nano X-ray Fluorescence. In K. Tsuji (Ed.), (pp. 45–46). Presented at the JST Symposium on “Micro and Trace X-ray Analysis,” 2-2-7, Higashi-Tenma, Kita-ku, Osaka, Japan: Sowa Information Control Center.
Vancouver
1.
De Samber B, Evens R, De Schamphelaere K, Masschaele B, Silversmit G, Schoonjans T, et al. Laboratory and Synchrotron Radiation Micro and Nano X-ray Fluorescence. In: Tsuji K, editor. 2-2-7, Higashi-Tenma, Kita-ku, Osaka, Japan: Sowa Information Control Center; 2009. p. 45–6.
MLA
De Samber, Björn, Roel Evens, Karel De Schamphelaere, et al. “Laboratory and Synchrotron Radiation Micro and Nano X-ray Fluorescence.” Ed. Kouichi Tsuji. 2-2-7, Higashi-Tenma, Kita-ku, Osaka, Japan: Sowa Information Control Center, 2009. 45–46. Print.