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An integrated targeted and untargeted approach for the analysis of ergot alkaloids in cereals using UHPLC - hybrid quadrupole time-of-flight mass spectrometry

(2015) WORLD MYCOTOXIN JOURNAL. 8(5). p.653-666
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Abstract
An ultra-high performance liquid chromatography hybrid quadrupole time of flight (Q-TOF) mass spectrometry (MS) method is described for the simultaneous quantitative determination of common ergot alkaloids and the screening, detection and identification of unexpected (less studied or novel) members of this class of toxic fungal secondary metabolites. The employed analytical strategy involves an untargeted data acquisition (consisting of full scan TOF MS survey and information dependent acquisition MS/MS scans) and the processing of data using both targeted and untargeted approaches. Method performance characteristics for the quantitative analysis of 6 common ergot alkaloids i.e. ergometrine, ergosine, ergotamine, ergocornine, ergocristine, ergokryptine and their corresponding epimers in rye were comparable to those previously reported for triple-quadrupole (QqQ) MS/MS. The method limits of quantification (LOQ) were in the range from 3 to 19 mu g/kg, and good linearity was observed for the different ergot alkaloids in the range from LOQ to 1000 mu g/kg. Furthermore, the method demonstrated good precision (relative standard deviations at 50 mu g/kg not higher than 14.6 and 16.2% for the intra-day and inter-day precision, respectively), and the trueness values at different concentration levels were all between 89 and 115%. The method was applied for the analysis of a set of 17 rye samples and demonstrated the presence of these ergot alkaloids in the range from <LOQ to 2,811 mu g/kg. Further mining of the same data based on a 'non-targeted peak finding' algorithm and the use of full MS and MS/MS accurate mass data allowed the detection and identification of 19 ergot alkaloids that are commonly not included in most analytical methods using QqQ instruments. Some of these alkaloids are reported for the first time in naturally contaminated samples.
Keywords
GRAINS, SAMPLES, EXCHANGE, EXTRACTION, MYCOTOXINS, PRODUCTS, RYE FLOUR, ELECTROSPRAY-IONIZATION, LIQUID-CHROMATOGRAPHY, LC-MS/MS METHOD, TripleTOF, IDA MS/MS, TOF-MS, ergot alkaloid identification, targeted and untargeted analysis

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Citation

Please use this url to cite or link to this publication:

Chicago
Arroyo Manzanares, Natalia, José Diana Di Mavungu, Valdet Uka, Laura Gámiz-Gracia, Ana M García-Campaña, and Sarah De Saeger. 2015. “An Integrated Targeted and Untargeted Approach for the Analysis of Ergot Alkaloids in Cereals Using UHPLC - Hybrid Quadrupole Time-of-flight Mass Spectrometry.” World Mycotoxin Journal 8 (5): 653–666.
APA
Arroyo Manzanares, N., Diana Di Mavungu, J., Uka, V., Gámiz-Gracia, L., García-Campaña, A. M., & De Saeger, S. (2015). An integrated targeted and untargeted approach for the analysis of ergot alkaloids in cereals using UHPLC - hybrid quadrupole time-of-flight mass spectrometry. WORLD MYCOTOXIN JOURNAL, 8(5), 653–666.
Vancouver
1.
Arroyo Manzanares N, Diana Di Mavungu J, Uka V, Gámiz-Gracia L, García-Campaña AM, De Saeger S. An integrated targeted and untargeted approach for the analysis of ergot alkaloids in cereals using UHPLC - hybrid quadrupole time-of-flight mass spectrometry. WORLD MYCOTOXIN JOURNAL. 2015;8(5):653–66.
MLA
Arroyo Manzanares, Natalia, José Diana Di Mavungu, Valdet Uka, et al. “An Integrated Targeted and Untargeted Approach for the Analysis of Ergot Alkaloids in Cereals Using UHPLC - Hybrid Quadrupole Time-of-flight Mass Spectrometry.” WORLD MYCOTOXIN JOURNAL 8.5 (2015): 653–666. Print.
@article{6985308,
  abstract     = {An ultra-high performance liquid chromatography hybrid quadrupole time of flight (Q-TOF) mass spectrometry (MS) method is described for the simultaneous quantitative determination of common ergot alkaloids and the screening, detection and identification of unexpected (less studied or novel) members of this class of toxic fungal secondary metabolites. The employed analytical strategy involves an untargeted data acquisition (consisting of full scan TOF MS survey and information dependent acquisition MS/MS scans) and the processing of data using both targeted and untargeted approaches. Method performance characteristics for the quantitative analysis of 6 common ergot alkaloids i.e. ergometrine, ergosine, ergotamine, ergocornine, ergocristine, ergokryptine and their corresponding epimers in rye were comparable to those previously reported for triple-quadrupole (QqQ) MS/MS. The method limits of quantification (LOQ) were in the range from 3 to 19 mu g/kg, and good linearity was observed for the different ergot alkaloids in the range from LOQ to 1000 mu g/kg. Furthermore, the method demonstrated good precision (relative standard deviations at 50 mu g/kg not higher than 14.6 and 16.2\% for the intra-day and inter-day precision, respectively), and the trueness values at different concentration levels were all between 89 and 115\%. The method was applied for the analysis of a set of 17 rye samples and demonstrated the presence of these ergot alkaloids in the range from {\textlangle}LOQ to 2,811 mu g/kg. Further mining of the same data based on a 'non-targeted peak finding' algorithm and the use of full MS and MS/MS accurate mass data allowed the detection and identification of 19 ergot alkaloids that are commonly not included in most analytical methods using QqQ instruments. Some of these alkaloids are reported for the first time in naturally contaminated samples.},
  author       = {Arroyo Manzanares, Natalia and Diana Di Mavungu, Jos{\'e} and Uka, Valdet and G{\'a}miz-Gracia, Laura and Garc{\'i}a-Campa{\~n}a, Ana M and De Saeger, Sarah},
  issn         = {1875-0710},
  journal      = {WORLD MYCOTOXIN JOURNAL},
  language     = {eng},
  number       = {5},
  pages        = {653--666},
  title        = {An integrated targeted and untargeted approach for the analysis of ergot alkaloids in cereals using UHPLC - hybrid quadrupole time-of-flight mass spectrometry},
  url          = {http://dx.doi.org/10.3920/WMJ2015.1900},
  volume       = {8},
  year         = {2015},
}

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