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A Top-Down Approach using X-ray Imaging Techniques: Instrumental Developments and Applications in Life Science

Björn De Samber UGent, Tom Schoonjans UGent, Geert Silversmit UGent, Bart Vekemans UGent, Laszlo Vincze UGent, Roel Evens UGent, Karel De Schamphelaere UGent, Colin Janssen UGent, Bert Masschaele UGent and Luc Van Hoorebeke UGent, et al. (2008) p.100-100
abstract
Synchrotron radiation X-ray fluorescence micro- and nanobeam techniques at second- and third generation SR sources offer the potential of non-destructive multi-element analysis down to trace concentration levels with unrivalled spatial resolution among X-ray based analytical techniques. At these sources, relative detection limits at the sub-ppm (fg/ng) level can be achieved. With respect to absolute detection limits (DL), sub-micron sized X-ray beams can offer DLs below 1 ag for the most efficiently excited transition elements, with a potential lateral resolution level better than 100 nm. These characteristics of micro/nanobeam SR-XRF allow spatially resolved multi-element determination of major, minor and trace constituents in microscopic sub areas and volumes within biological specimens in an essentially non-destructive/non-invasive manner. However, the complexity of performing such an experiment is often quite considerable, involving dedicated sample preparation, transportation towards and experimenting at the synchrotron facility, installing an appropriate experimental set-up and performing a thorough data analysis on large amounts of spectral data. The ecotoxicological research on Daphnia magna, a frequently used model organism for investigating the mechanisms of toxicity of metals, has often been difficult because many analytical techniques are not able to investigate trace metal distributions in a spatially resolved manner at a (sub)microscopic resolution. As illustrated by this presentation, SR-XRF microanalysis allows to fill this gap and moreover, due to the variety in sizes of X-ray beams available, this research can be performed from the organism level towards the tissue and cellular level, representing a top-down approach.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
micro-XRF, Daphnia magna, ecotoxicology, synchrotron
pages
1 pages
conference name
2008 Denver X-ray conference
conference location
Denver Marriot Tech Center Hotel
conference start
2008-08-04
conference end
2008-08-08
language
English
UGent publication?
yes
classification
C3
id
698487
handle
http://hdl.handle.net/1854/LU-698487
date created
2009-06-15 10:22:29
date last changed
2009-06-26 16:12:12
@inproceedings{698487,
  abstract     = {Synchrotron radiation X-ray fluorescence micro- and nanobeam techniques at second- and third generation SR sources offer the potential of non-destructive multi-element analysis down to trace concentration levels with unrivalled spatial resolution among X-ray based analytical techniques. At these sources, relative detection limits at the sub-ppm (fg/ng) level can be achieved. With respect to absolute detection limits (DL), sub-micron sized X-ray beams can offer DLs below 1 ag for the most efficiently excited transition elements, with a potential lateral resolution level better than 100 nm.

These characteristics of micro/nanobeam SR-XRF allow spatially resolved multi-element determination of major, minor and trace constituents in microscopic sub areas and volumes within biological specimens in an essentially non-destructive/non-invasive manner. However, the complexity of performing such an experiment is often quite considerable, involving dedicated sample preparation, transportation towards and experimenting at the synchrotron facility, installing an appropriate experimental set-up and performing a thorough data analysis on large amounts of spectral data.

The ecotoxicological research on Daphnia magna, a frequently used model organism for investigating the mechanisms of toxicity of metals, has often been difficult because many analytical techniques are not able to investigate trace metal distributions in a spatially resolved manner at a (sub)microscopic resolution. As illustrated by this presentation, SR-XRF microanalysis allows to fill this gap and moreover, due to the variety in sizes of X-ray beams available, this research can be performed from the organism level towards the tissue and cellular level, representing a top-down approach.},
  author       = {De Samber, Bj{\"o}rn and Schoonjans, Tom and Silversmit, Geert and Vekemans, Bart and Vincze, Laszlo and Evens, Roel and De Schamphelaere, Karel and Janssen, Colin and Masschaele, Bert and Van Hoorebeke, Luc and Bohic, Sylvain and Rickers, Karen and Falkenberg, Gerald},
  keyword      = {micro-XRF,Daphnia magna,ecotoxicology,synchrotron},
  language     = {eng},
  location     = {Denver Marriot Tech Center Hotel},
  pages        = {100--100},
  title        = {A Top-Down Approach using X-ray Imaging Techniques: Instrumental Developments and Applications in Life Science},
  year         = {2008},
}

Chicago
De Samber, Björn, Tom Schoonjans, Geert Silversmit, Bart Vekemans, Laszlo Vincze, Roel Evens, Karel De Schamphelaere, et al. 2008. “A Top-Down Approach Using X-ray Imaging Techniques: Instrumental Developments and Applications in Life Science.” In , 100–100.
APA
De Samber, B., Schoonjans, T., Silversmit, G., Vekemans, B., Vincze, L., Evens, R., De Schamphelaere, K., et al. (2008). A Top-Down Approach using X-ray Imaging Techniques: Instrumental Developments and Applications in Life Science (pp. 100–100). Presented at the 2008 Denver X-ray conference.
Vancouver
1.
De Samber B, Schoonjans T, Silversmit G, Vekemans B, Vincze L, Evens R, et al. A Top-Down Approach using X-ray Imaging Techniques: Instrumental Developments and Applications in Life Science. 2008. p. 100–100.
MLA
De Samber, Björn, Tom Schoonjans, Geert Silversmit, et al. “A Top-Down Approach Using X-ray Imaging Techniques: Instrumental Developments and Applications in Life Science.” 2008. 100–100. Print.