Advanced search
1 file | 1.18 MB

Quorum sensing peptides selectively penetrate the blood-brain barrier

(2015) PLOS ONE. 10(11).
Author
Organization
Abstract
Bacteria communicate with each other by the use of signaling molecules, a process called 'quorum sensing'. One group of quorum sensing molecules includes the oligopeptides, which are mainly produced by Gram-positive bacteria. Recently, these quorum sensing peptides were found to biologically influence mammalian cells, promoting i.a. metastasis of cancer cells. Moreover, it was found that bacteria can influence different central nervous system related disorders as well, e.g. anxiety, depression and autism. Research currently focuses on the role of bacterial metabolites in this bacteria-brain interaction, with the role of the quorum sensing peptides not yet known. Here, three chemically diverse quorum sensing peptides were investigated for their brain influx (multiple time regression technique) and efflux properties in an in vivo mouse model (ICR-CD-1) to determine blood-brain transfer properties: PhrCACET1 demonstrated comparatively a very high initial influx into the mouse brain (Kin = 20.87 μl/(g×min)), while brain penetrabilities of BIP-2 and PhrANTH2 were found to be low (Kin = 2.68 μl/(g×min)) and very low (Kin = 0.18 μl/(g×min)), respectively. All three quorum sensing peptides were metabolically stable in plasma (in vitro) during the experimental time frame and no significant brain efflux was observed. Initial tissue distribution data showed remarkably high liver accumulation of BIP-2 as well. Our results thus support the potential role of some quorum sensing peptides in different neurological disorders, thereby enlarging our knowledge about the microbiome-brain axis.
Keywords
Quorum sensing peptides, blood-brain barrier, microbiome, Permeability, neurological diseases, STREPTOCOCCUS-PNEUMONIAE, GUT-BRAIN, MICROBIOME, TRANSPORT, COMMUNICATION, DEPRESSION, ANXIETY, CROSS, RADIOPHARMACEUTICALS, QUANTIFICATION

Downloads

  • pone.0142071.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.18 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Wynendaele, Evelien, Frederick Verbeke, Sofie Stalmans, Bert Gevaert, Yorick Janssens, Christophe Van De Wiele, Kathelijne Peremans, Christian Burvenich, and Bart De Spiegeleer. 2015. “Quorum Sensing Peptides Selectively Penetrate the Blood-brain Barrier.” Plos One 10 (11).
APA
Wynendaele, E., Verbeke, F., Stalmans, S., Gevaert, B., Janssens, Y., Van De Wiele, C., Peremans, K., et al. (2015). Quorum sensing peptides selectively penetrate the blood-brain barrier. PLOS ONE, 10(11).
Vancouver
1.
Wynendaele E, Verbeke F, Stalmans S, Gevaert B, Janssens Y, Van De Wiele C, et al. Quorum sensing peptides selectively penetrate the blood-brain barrier. PLOS ONE. 2015;10(11).
MLA
Wynendaele, Evelien, Frederick Verbeke, Sofie Stalmans, et al. “Quorum Sensing Peptides Selectively Penetrate the Blood-brain Barrier.” PLOS ONE 10.11 (2015): n. pag. Print.
@article{6983661,
  abstract     = {Bacteria communicate with each other by the use of signaling molecules, a process called 'quorum sensing'. One group of quorum sensing molecules includes the oligopeptides, which are mainly produced by Gram-positive bacteria. Recently, these quorum sensing peptides were found to biologically influence mammalian cells, promoting i.a. metastasis of cancer cells. Moreover, it was found that bacteria can influence different central nervous system related disorders as well, e.g. anxiety, depression and autism. Research currently focuses on the role of bacterial metabolites in this bacteria-brain interaction, with the role of the quorum sensing peptides not yet known. Here, three chemically diverse quorum sensing peptides were investigated for their brain influx (multiple time regression technique) and efflux properties in an in vivo mouse model (ICR-CD-1) to determine blood-brain transfer properties: PhrCACET1 demonstrated comparatively a very high initial influx into the mouse brain (Kin = 20.87 \ensuremath{\mu}l/(g{\texttimes}min)), while brain penetrabilities of BIP-2 and PhrANTH2 were found to be low (Kin = 2.68 \ensuremath{\mu}l/(g{\texttimes}min)) and very low (Kin = 0.18 \ensuremath{\mu}l/(g{\texttimes}min)), respectively. All three quorum sensing peptides were metabolically stable in plasma (in vitro) during the experimental time frame and no significant brain efflux was observed. Initial tissue distribution data showed remarkably high liver accumulation of BIP-2 as well. Our results thus support the potential role of some quorum sensing peptides in different neurological disorders, thereby enlarging our knowledge about the microbiome-brain axis.},
  articleno    = {e0142071},
  author       = {Wynendaele, Evelien and Verbeke, Frederick and Stalmans, Sofie and Gevaert, Bert and Janssens, Yorick and Van De Wiele, Christophe and Peremans, Kathelijne and Burvenich, Christian and De Spiegeleer, Bart},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {11},
  pages        = {15},
  title        = {Quorum sensing peptides selectively penetrate the blood-brain barrier},
  url          = {http://dx.doi.org/10.1371/journal.pone.0142071},
  volume       = {10},
  year         = {2015},
}

Altmetric
View in Altmetric
Web of Science
Times cited: