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Staphylococcus devriesei sp. nov., isolated from teat apices and milk of dairy cows

Karlien Supré UGent, Sarne De Vliegher UGent, Ilse Cleenwerck UGent, Katrien Engelbeen UGent, Stefanie Van Trappen UGent, Sofie Piepers UGent, Otlis Sampimon, Ruth Zadoks, Paul De Vos UGent and Freddy Haesebrouck UGent (2010) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 60(12). p.2739-2744
abstract
Ten non-motile, Gram-stain-positive, coagulase-negative staphylococci were isolated from bovine milk and teat apices. All isolates were catalase-positive, with anteiso-C-15:0, iso-C-15:0, anteiso-C-17:0, iso-C-17:0 and C-18:0 as predominant fatty acids and diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. The results of sequence analysis of the 16S rRNA gene and four housekeeping genes (rpoB, hsp60, tuf and dnaJ) in combination with tRNA-intergenic spacer length analysis showed that the isolates form a separate branch within the genus Staphylococcus. Based on 16S rRNA gene sequencing, the phylogenetically most closely related species are Staphylococcus haemolyticus, S. hominis and S. lugdunensis, with >98.7% sequence similarity. The DNA G+C content varies from 33.3 to 33.7 mol%, and DNA-DNA hybridization with the nearest neighbours, based on 16S rRNA gene sequences, confirmed that the isolates represent a novel Staphylococcus species. All isolates induced a small zone of complete haemolysis on Columbia agar with 5% sheep blood and exhibited a homogeneous biochemical fingerprint that is discriminative from the phylogenetically most closely related species. Based on these results, it is proposed to classify the ten isolates as Staphylococcus devriesei sp. nov., with strain KS-SP 60(T) (=LMG 25332(T) =CCUG 58238(T)) as the type strain.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CHROMOGENES, HYBRIDIZATION, SPECIES IDENTIFICATION, COAGULASE-NEGATIVE STAPHYLOCOCCI, DEOXYRIBONUCLEIC-ACID, MASTITIS PATHOGENS, LIPID-COMPOSITION, CLASSIFICATION, FATTY-ACIDS, PREVALENCE
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
60
issue
12
pages
2739 - 2744
Web of Science type
Article
Web of Science id
000286169100009
JCR category
MICROBIOLOGY
JCR impact factor
1.93 (2010)
JCR rank
68/103 (2010)
JCR quartile
3 (2010)
ISSN
1466-5026
DOI
10.1099/ijs.0.015982-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
698200
handle
http://hdl.handle.net/1854/LU-698200
date created
2009-06-15 09:06:16
date last changed
2011-05-27 16:25:15
@article{698200,
  abstract     = {Ten non-motile, Gram-stain-positive, coagulase-negative staphylococci were isolated from bovine milk and teat apices. All isolates were catalase-positive, with anteiso-C-15:0, iso-C-15:0, anteiso-C-17:0, iso-C-17:0 and C-18:0 as predominant fatty acids and diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. The results of sequence analysis of the 16S rRNA gene and four housekeeping genes (rpoB, hsp60, tuf and dnaJ) in combination with tRNA-intergenic spacer length analysis showed that the isolates form a separate branch within the genus Staphylococcus. Based on 16S rRNA gene sequencing, the phylogenetically most closely related species are Staphylococcus haemolyticus, S. hominis and S. lugdunensis, with {\textrangle}98.7\% sequence similarity. The DNA G+C content varies from 33.3 to 33.7 mol\%, and DNA-DNA hybridization with the nearest neighbours, based on 16S rRNA gene sequences, confirmed that the isolates represent a novel Staphylococcus species. All isolates induced a small zone of complete haemolysis on Columbia agar with 5\% sheep blood and exhibited a homogeneous biochemical fingerprint that is discriminative from the phylogenetically most closely related species. Based on these results, it is proposed to classify the ten isolates as Staphylococcus devriesei sp. nov., with strain KS-SP 60(T) (=LMG 25332(T) =CCUG 58238(T)) as the type strain.},
  author       = {Supr{\'e}, Karlien and De Vliegher, Sarne and Cleenwerck, Ilse and Engelbeen, Katrien and Van Trappen, Stefanie and Piepers, Sofie and Sampimon, Otlis and Zadoks, Ruth and De Vos, Paul and Haesebrouck, Freddy},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {CHROMOGENES,HYBRIDIZATION,SPECIES IDENTIFICATION,COAGULASE-NEGATIVE STAPHYLOCOCCI,DEOXYRIBONUCLEIC-ACID,MASTITIS PATHOGENS,LIPID-COMPOSITION,CLASSIFICATION,FATTY-ACIDS,PREVALENCE},
  language     = {eng},
  number       = {12},
  pages        = {2739--2744},
  title        = {Staphylococcus devriesei sp. nov., isolated from teat apices and milk of dairy cows},
  url          = {http://dx.doi.org/10.1099/ijs.0.015982-0},
  volume       = {60},
  year         = {2010},
}

Chicago
Supré, Karlien, Sarne De Vliegher, Ilse Cleenwerck, Katrien Engelbeen, Stefanie Van Trappen, Sofie Piepers, Otlis Sampimon, Ruth Zadoks, Paul De Vos, and Freddy Haesebrouck. 2010. “Staphylococcus Devriesei Sp. Nov., Isolated from Teat Apices and Milk of Dairy Cows.” International Journal of Systematic and Evolutionary Microbiology 60 (12): 2739–2744.
APA
Supré, K., De Vliegher, S., Cleenwerck, I., Engelbeen, K., Van Trappen, S., Piepers, S., Sampimon, O., et al. (2010). Staphylococcus devriesei sp. nov., isolated from teat apices and milk of dairy cows. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 60(12), 2739–2744.
Vancouver
1.
Supré K, De Vliegher S, Cleenwerck I, Engelbeen K, Van Trappen S, Piepers S, et al. Staphylococcus devriesei sp. nov., isolated from teat apices and milk of dairy cows. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2010;60(12):2739–44.
MLA
Supré, Karlien, Sarne De Vliegher, Ilse Cleenwerck, et al. “Staphylococcus Devriesei Sp. Nov., Isolated from Teat Apices and Milk of Dairy Cows.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 60.12 (2010): 2739–2744. Print.