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Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Vanessa Meganck (UGent) , Bruno Goddeeris (UGent) , S De Campeneere, Miel Hostens (UGent) , Mieke Van Eetvelde (UGent) , Sofie Piepers (UGent) , Eric Cox (UGent) and Geert Opsomer (UGent)
(2015) JOURNAL OF DAIRY SCIENCE. 98(10). p.6782-6791
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Abstract
In neonatal calves, the ingestion of colostrum is imperative for preventing infectious diseases. Investigations into the transfer of passive immunity of cattle have primarily focused on the importance of colostral immunoglobulins, with a recent increase in focus on understanding the role of colostral leukocytes. The main objective of the present study was to measure the influence of parity, body condition score, serum nonesterified fatty acids, and serum beta-hydroxybutyrate concentrations of periparturient cows on phenotype and mitogen-and antigen-induced proliferative capacity of bovine colostral leukocytes. Holstein-Friesian cows (n = 141) were intramuscularly vaccinated at 60 and 30 d before the expected parturition date with a tetanus toxoid vaccine. Of these 141 animals, 28 primiparous and 72 multiparous cows were sampled immediately. after parturition. Colostrum mononuclear cell populations were identified by flow cytometry using bovine cluster of differentiation markers, and the proliferative capacity of these cells was determined using a H-3-thymidine proliferation assay. Under-conditioned cows had a significantly higher percentage of colostral macrophages than normal-conditioned animals, whereas over-conditioned cows had significantly more colostral B-lymphocytes. Serum beta-hydroxybutyrate was significantly associated with higher numbers of colostral T-lymphocytes and macrophages. Heifers had significantly higher mitogen- and antigen-induced proliferation of their colostral leukocytes than third parity or older cows. In conclusion, body condition score, parity, and serum beta-hydroxybutyrate concentration of periparturient high-yielding dairy cows were shown to influence the number of colostral macrophages or the mitogen-and antigen-induced proliferation of colostral leukocytes, possibly influencing the cellular immunity of the newborn calf.
Keywords
negative energy balance, leukocytes, BOVINE POLYMORPHONUCLEAR LEUKOCYTES, MAMMARY-GLAND SECRETIONS, MILK T-LYMPHOCYTES, PERIPHERAL-BLOOD, PERIPARTURIENT PERIOD, NEONATAL CALVES, NEWBORN CALVES, dairy cows, colostrum, NEUTROPHIL FUNCTION, MITOGENIC RESPONSES, IGG CONCENTRATION

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Chicago
Meganck, Vanessa, Bruno Goddeeris, S De Campeneere, Miel Hostens, Mieke Van Eetvelde, Sofie Piepers, Eric Cox, and Geert Opsomer. 2015. “Effect of Β-hydroxybutyric Acid, Parity, and Body Condition Score on Phenotype and Proliferative Capacity of Colostral Mononuclear Leukocytes of High-yielding Dairy Cows.” Journal of Dairy Science 98 (10): 6782–6791.
APA
Meganck, V., Goddeeris, B., De Campeneere, S., Hostens, M., Van Eetvelde, M., Piepers, S., Cox, E., et al. (2015). Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows. JOURNAL OF DAIRY SCIENCE, 98(10), 6782–6791.
Vancouver
1.
Meganck V, Goddeeris B, De Campeneere S, Hostens M, Van Eetvelde M, Piepers S, et al. Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows. JOURNAL OF DAIRY SCIENCE. 2015;98(10):6782–91.
MLA
Meganck, Vanessa, Bruno Goddeeris, S De Campeneere, et al. “Effect of Β-hydroxybutyric Acid, Parity, and Body Condition Score on Phenotype and Proliferative Capacity of Colostral Mononuclear Leukocytes of High-yielding Dairy Cows.” JOURNAL OF DAIRY SCIENCE 98.10 (2015): 6782–6791. Print.
@article{6977049,
  abstract     = {In neonatal calves, the ingestion of colostrum is imperative for preventing infectious diseases. Investigations into the transfer of passive immunity of cattle have primarily focused on the importance of colostral immunoglobulins, with a recent increase in focus on understanding the role of colostral leukocytes. The main objective of the present study was to measure the influence of parity, body condition score, serum nonesterified fatty acids, and serum beta-hydroxybutyrate concentrations of periparturient cows on phenotype and mitogen-and antigen-induced proliferative capacity of bovine colostral leukocytes. Holstein-Friesian cows (n = 141) were intramuscularly vaccinated at 60 and 30 d before the expected parturition date with a tetanus toxoid vaccine. Of these 141 animals, 28 primiparous and 72 multiparous cows were sampled immediately. after parturition. Colostrum mononuclear cell populations were identified by flow cytometry using bovine cluster of differentiation markers, and the proliferative capacity of these cells was determined using a H-3-thymidine proliferation assay. Under-conditioned cows had a significantly higher percentage of colostral macrophages than normal-conditioned animals, whereas over-conditioned cows had significantly more colostral B-lymphocytes. Serum beta-hydroxybutyrate was significantly associated with higher numbers of colostral T-lymphocytes and macrophages. Heifers had significantly higher mitogen- and antigen-induced proliferation of their colostral leukocytes than third parity or older cows. In conclusion, body condition score, parity, and serum beta-hydroxybutyrate concentration of periparturient high-yielding dairy cows were shown to influence the number of colostral macrophages or the mitogen-and antigen-induced proliferation of colostral leukocytes, possibly influencing the cellular immunity of the newborn calf.},
  author       = {Meganck, Vanessa and Goddeeris, Bruno and De Campeneere, S and Hostens, Miel and Van Eetvelde, Mieke and Piepers, Sofie and Cox, Eric and Opsomer, Geert},
  issn         = {0022-0302},
  journal      = {JOURNAL OF DAIRY SCIENCE},
  keyword      = {negative energy balance,leukocytes,BOVINE POLYMORPHONUCLEAR LEUKOCYTES,MAMMARY-GLAND SECRETIONS,MILK T-LYMPHOCYTES,PERIPHERAL-BLOOD,PERIPARTURIENT PERIOD,NEONATAL CALVES,NEWBORN CALVES,dairy cows,colostrum,NEUTROPHIL FUNCTION,MITOGENIC RESPONSES,IGG CONCENTRATION},
  language     = {eng},
  number       = {10},
  pages        = {6782--6791},
  title        = {Effect of \ensuremath{\beta}-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows},
  url          = {http://dx.doi.org/10.3168/jds.2014-8780},
  volume       = {98},
  year         = {2015},
}

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