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Steroids affect gene expression, ciliary activity, glucose uptake, progesterone receptor expression and immunoreactive steroidogenic protein expression in equine oviduct explants in vitro

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grant GO35511N Research Foundation Flanders
Project
grant 1.1.425.10.N.00 Research Foundation Flanders
Abstract
The oviduct undergoes dramatic functional and morphological changes throughout the estrous cycle of the mare. To unravel the effects of steroids on the morphology, functionality and gene expression of the equine oviduct, an in vitro oviduct explant culture system was stimulated with physiological concentrations of progesterone and 17β-oestradiol. Four conditions were compared: unsupplemented preovulatory explants, preovulatory explants which were stimulated with postovulatory hormone concentrations, unsupplemented postovulatory explants and postovulatory explants which were stimulated with preovulatory hormone concentrations. The modulating effects of both steroids on oviduct explants at different levels were investigated: 1) ciliary activity, 2) glucose consumption and lactate production pattern, 3) ultrastructure, 4) mRNA expression of embryotrophic genes, 5) steroidogenic capacities of oviductal explants, and 6) progesterone receptor expression. The present paper shows that the equine oviduct is an organ with potential steroidogenic capacities which is highly responsive to local changes in progesterone and 17β-oestradiol concentrations at the level of morphology, functionality and gene expression of the oviduct. These data provide a basis to study the importance of endocrine and paracrine signalling during early embryonic development in the horse.
Keywords
mare, StAR, cytochrome P450scc, aromatase, 3-beta-HSD, steroidogenesis

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Chicago
Nelis, Hilde, Bartosz Wojciechowicz, Anita Franczak, Bart Leemans, Katharina D’Herde, Karen Goossens, Pieter Cornillie, Luc Peelman, Ann Van Soom, and Katrien Smits. 2016. “Steroids Affect Gene Expression, Ciliary Activity, Glucose Uptake, Progesterone Receptor Expression and Immunoreactive Steroidogenic Protein Expression in Equine Oviduct Explants in Vitro.” Reproduction Fertility and Development 28 (12): 1926–1944.
APA
Nelis, Hilde, Wojciechowicz, B., Franczak, A., Leemans, B., D’Herde, K., Goossens, K., Cornillie, P., et al. (2016). Steroids affect gene expression, ciliary activity, glucose uptake, progesterone receptor expression and immunoreactive steroidogenic protein expression in equine oviduct explants in vitro. REPRODUCTION FERTILITY AND DEVELOPMENT, 28(12), 1926–1944.
Vancouver
1.
Nelis H, Wojciechowicz B, Franczak A, Leemans B, D’Herde K, Goossens K, et al. Steroids affect gene expression, ciliary activity, glucose uptake, progesterone receptor expression and immunoreactive steroidogenic protein expression in equine oviduct explants in vitro. REPRODUCTION FERTILITY AND DEVELOPMENT. 2016;28(12):1926–44.
MLA
Nelis, Hilde, Bartosz Wojciechowicz, Anita Franczak, et al. “Steroids Affect Gene Expression, Ciliary Activity, Glucose Uptake, Progesterone Receptor Expression and Immunoreactive Steroidogenic Protein Expression in Equine Oviduct Explants in Vitro.” REPRODUCTION FERTILITY AND DEVELOPMENT 28.12 (2016): 1926–1944. Print.
@article{6975828,
  abstract     = {The oviduct undergoes dramatic functional and morphological changes throughout the estrous cycle of the mare. To unravel the effects of steroids on the morphology, functionality and gene expression of the equine oviduct, an in vitro oviduct explant culture system was stimulated with physiological concentrations of progesterone and 17\ensuremath{\beta}-oestradiol. Four conditions were compared: unsupplemented preovulatory explants, preovulatory explants which were stimulated with postovulatory hormone concentrations, unsupplemented postovulatory explants and postovulatory explants which were stimulated with preovulatory hormone concentrations. The modulating effects of both steroids on oviduct explants at different levels were investigated: 1) ciliary activity, 2) glucose consumption and lactate production pattern, 3) ultrastructure, 4) mRNA expression of embryotrophic genes, 5) steroidogenic capacities of oviductal explants, and 6) progesterone receptor expression.
The present paper shows that the equine oviduct is an organ with potential steroidogenic capacities which is highly responsive to local changes in progesterone and 17\ensuremath{\beta}-oestradiol concentrations at the level of morphology, functionality and gene expression of the oviduct. These data provide a basis to study the importance of endocrine and paracrine signalling during early embryonic development in the horse.},
  author       = {Nelis, Hilde and Wojciechowicz, Bartosz and Franczak, Anita and Leemans, Bart and D'Herde, Katharina and Goossens, Karen and Cornillie, Pieter and Peelman, Luc and Van Soom, Ann and Smits, Katrien},
  issn         = {1031-3613},
  journal      = {REPRODUCTION FERTILITY AND DEVELOPMENT},
  keyword      = {mare,StAR,cytochrome P450scc,aromatase,3-beta-HSD,steroidogenesis},
  language     = {eng},
  number       = {12},
  pages        = {1926--1944},
  title        = {Steroids affect gene expression, ciliary activity, glucose uptake, progesterone receptor expression and immunoreactive steroidogenic protein expression in equine oviduct explants in vitro},
  url          = {http://dx.doi.org/10.1071/RD15044},
  volume       = {28},
  year         = {2016},
}

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