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Steroids in the equine oviduct: synthesis, local concentrations and receptor expression

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grant 1.1.425.10.N.00 of the Research Foundation Flanders
Project
grant GO35511N of the Research Foundation Flanders
Abstract
Steroids play an important role in mammalian reproduction and early pregnancy. Whereas systemic changes in steroid concentrations have been well documented, it is not clear how these correlate with local steroid concentrations in the genital tract. We hypothesized that, in the horse, the pre-implantation embryo may be subjected to high local steroid concentrations for several days. Therefore, we measured progesterone, 17-hydroxyprogesterone, 17β-oestradiol, testosterone and 17α-testosterone concentrations in equine oviductal tissue by means of ultra-high performance liquid chromatography coupled to tandem mass spectrometry and progesterone, 17β-oestradiol, oestrone and testosterone in oviduct fluid using radioimmunoassay, with reference to cycle stage and side of ovulation. Progesterone concentrations were high in oviductal tissue and fluid ipsilateral to the ovulation side during diestrus, whereas other steroid hormone concentrations were not influenced by the side of ovulation. These results suggest that the high ipsilateral progesterone concentration is induced by 1) the contribution from follicular fluid in the oviduct and the diffusion of follicular fluid steroids after ovulation; 2) a local transfer of steroids via blood or lymph, 3) local synthesis of progesterone in the oviduct, as evidenced by the expression of steroidogenic enzymes and, 4) the paracrine contribution from follicular cells. These data provide a basis to study the importance of endocrine and paracrine signalling during early embryonic development in the horse.
Keywords
cytochrome P450scc, aromatase, follicular fluid, 3 beta-hydroxysteroid dehydrogenase, mare, oviductal fluid, progesterone receptor, serum, steroidogenesis, steroidogenic acute regulatory protein, IN-VITRO, EARLY-PREGNANCY, BOVINE OVIDUCT, ESTROUS-CYCLE, PROGESTERONE-RECEPTOR, OVARIAN-STEROIDS, EPITHELIAL-CELLS, SPERM TRANSPORT, GRANULOSA-CELLS, PERITONEAL-CAVITY

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Citation

Please use this url to cite or link to this publication:

Chicago
Nelis, Hilde, Julie Vanden Bussche, Bartosz Wojciechowicz, Anita Franczak, Lynn Vanhaecke, Bart Leemans, Pieter Cornillie, Luc Peelman, Ann Van Soom, and Katrien Smits. 2016. “Steroids in the Equine Oviduct: Synthesis, Local Concentrations and Receptor Expression.” Reproduction Fertility and Development 28 (9): 1390–1404.
APA
Nelis, Hilde, Vanden Bussche, J., Wojciechowicz, B., Franczak, A., Vanhaecke, L., Leemans, B., Cornillie, P., et al. (2016). Steroids in the equine oviduct: synthesis, local concentrations and receptor expression. REPRODUCTION FERTILITY AND DEVELOPMENT, 28(9), 1390–1404.
Vancouver
1.
Nelis H, Vanden Bussche J, Wojciechowicz B, Franczak A, Vanhaecke L, Leemans B, et al. Steroids in the equine oviduct: synthesis, local concentrations and receptor expression. REPRODUCTION FERTILITY AND DEVELOPMENT. 2016;28(9):1390–404.
MLA
Nelis, Hilde, Julie Vanden Bussche, Bartosz Wojciechowicz, et al. “Steroids in the Equine Oviduct: Synthesis, Local Concentrations and Receptor Expression.” REPRODUCTION FERTILITY AND DEVELOPMENT 28.9 (2016): 1390–1404. Print.
@article{6975806,
  abstract     = {Steroids play an important role in mammalian reproduction and early pregnancy. Whereas systemic changes in steroid concentrations have been well documented, it is not clear how these correlate with local steroid concentrations in the genital tract. We hypothesized that, in the horse, the pre-implantation embryo may be subjected to high local steroid concentrations for several days. Therefore, we measured progesterone, 17-hydroxyprogesterone, 17\ensuremath{\beta}-oestradiol, testosterone and 17\ensuremath{\alpha}-testosterone concentrations in equine oviductal tissue by means of ultra-high performance liquid chromatography coupled to tandem mass spectrometry and progesterone, 17\ensuremath{\beta}-oestradiol, oestrone and testosterone in oviduct fluid using radioimmunoassay, with reference to cycle stage and side of ovulation. Progesterone concentrations were high in oviductal tissue and fluid ipsilateral to the ovulation side during diestrus, whereas other steroid hormone concentrations were not influenced by the side of ovulation. These results suggest that the high ipsilateral progesterone concentration is induced by 1) the contribution from follicular fluid in the oviduct and the diffusion of follicular fluid steroids after ovulation; 2) a local transfer of steroids via blood or lymph, 3) local synthesis of progesterone in the oviduct, as evidenced by the expression of steroidogenic enzymes and, 4) the paracrine contribution from follicular cells. These data provide a basis to study the importance of endocrine and paracrine signalling during early embryonic development in the horse.},
  author       = {Nelis, Hilde and Vanden Bussche, Julie and Wojciechowicz, Bartosz and Franczak, Anita and Vanhaecke, Lynn and Leemans, Bart and Cornillie, Pieter and Peelman, Luc and Van Soom, Ann and Smits, Katrien},
  issn         = {1031-3613},
  journal      = {REPRODUCTION FERTILITY AND DEVELOPMENT},
  keyword      = {cytochrome P450scc,aromatase,follicular fluid,3 beta-hydroxysteroid dehydrogenase,mare,oviductal fluid,progesterone receptor,serum,steroidogenesis,steroidogenic acute regulatory protein,IN-VITRO,EARLY-PREGNANCY,BOVINE OVIDUCT,ESTROUS-CYCLE,PROGESTERONE-RECEPTOR,OVARIAN-STEROIDS,EPITHELIAL-CELLS,SPERM TRANSPORT,GRANULOSA-CELLS,PERITONEAL-CAVITY},
  language     = {eng},
  number       = {9},
  pages        = {1390--1404},
  title        = {Steroids in the equine oviduct: synthesis, local concentrations and receptor expression},
  url          = {http://dx.doi.org/10.1071/RD14483},
  volume       = {28},
  year         = {2016},
}

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