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Light-inducible and tissue-specific expression of a chimaeric gene under control of the 5'-flanking sequence of a pea chlorophyll a/b-binding protein gene

(1985) EMBO JOURNAL. 4(11). p.2723-2729
Author
Organization
Abstract
We have investigated the regulatory functions of the 5'-flanking sequences of a chlorophyll a/b-binding protein gene from Pisum sativum, using the neomycin phosphotransferase (II) activity from Tn5 as an enzymatic reporter. We show that 0.4 kb of the upstream flanking sequences of this gene are sufficient for both organ-specific and light-regulated expression of our chimaeric constructs in transformed tobacco plants. In addition, we show that sequences farther upstream have a significant influence on the level of transcription of these constructions.
Keywords
light-inducible promoter, chimaeric gene, tissue-specific expression, Plant cell transformation

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Citation

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MLA
Simpson, June et al. “Light-inducible and Tissue-specific Expression of a Chimaeric Gene Under Control of the 5’-flanking Sequence of a Pea Chlorophyll A/b-binding Protein Gene.” EMBO JOURNAL 4.11 (1985): 2723–2729. Print.
APA
Simpson, J., Timko, M. P., Cashmore, A. R., Schell, J., Van Montagu, M., & Herrera-Estrella, L. (1985). Light-inducible and tissue-specific expression of a chimaeric gene under control of the 5’-flanking sequence of a pea chlorophyll a/b-binding protein gene. EMBO JOURNAL, 4(11), 2723–2729.
Chicago author-date
Simpson, June, Michael P Timko, Anthony R Cashmore, Jeff Schell, Marc Van Montagu, and Luis Herrera-Estrella. 1985. “Light-inducible and Tissue-specific Expression of a Chimaeric Gene Under Control of the 5’-flanking Sequence of a Pea Chlorophyll A/b-binding Protein Gene.” Embo Journal 4 (11): 2723–2729.
Chicago author-date (all authors)
Simpson, June, Michael P Timko, Anthony R Cashmore, Jeff Schell, Marc Van Montagu, and Luis Herrera-Estrella. 1985. “Light-inducible and Tissue-specific Expression of a Chimaeric Gene Under Control of the 5’-flanking Sequence of a Pea Chlorophyll A/b-binding Protein Gene.” Embo Journal 4 (11): 2723–2729.
Vancouver
1.
Simpson J, Timko MP, Cashmore AR, Schell J, Van Montagu M, Herrera-Estrella L. Light-inducible and tissue-specific expression of a chimaeric gene under control of the 5’-flanking sequence of a pea chlorophyll a/b-binding protein gene. EMBO JOURNAL. 1985;4(11):2723–9.
IEEE
[1]
J. Simpson, M. P. Timko, A. R. Cashmore, J. Schell, M. Van Montagu, and L. Herrera-Estrella, “Light-inducible and tissue-specific expression of a chimaeric gene under control of the 5’-flanking sequence of a pea chlorophyll a/b-binding protein gene,” EMBO JOURNAL, vol. 4, no. 11, pp. 2723–2729, 1985.
@article{6974824,
  abstract     = {We have investigated the regulatory functions of the 5'-flanking sequences of a chlorophyll a/b-binding protein gene from Pisum sativum, using the neomycin phosphotransferase (II) activity from Tn5 as an enzymatic reporter. We show that 0.4 kb of the upstream flanking sequences of this gene are sufficient for both organ-specific and light-regulated expression of our chimaeric constructs in transformed tobacco plants. In addition, we show that sequences farther upstream have a significant influence on the level of transcription of these constructions.},
  author       = {Simpson, June and Timko, Michael P and Cashmore, Anthony R and Schell, Jeff and Van Montagu, Marc and Herrera-Estrella, Luis},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  keywords     = {light-inducible promoter,chimaeric gene,tissue-specific expression,Plant cell transformation},
  language     = {eng},
  number       = {11},
  pages        = {2723--2729},
  title        = {Light-inducible and tissue-specific expression of a chimaeric gene under control of the 5'-flanking sequence of a pea chlorophyll a/b-binding protein gene},
  url          = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC554570/},
  volume       = {4},
  year         = {1985},
}