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Light regulation of plant gene expression by an upstream enhancer-like element

(1985) NATURE. 318(6046). p.579-582
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Abstract
Light regulates many varied physiological and developmental phenomena during plant growth and differentiation, including the formation of a photosynthetically competent chloroplast from a proplastid1. The expression of ribulose 1,5-bisphosphate carboxylase small subunit (rbcS) genes is regulated by light in a development- and tissue-specific manner2,3. In some plant species, phytochrome has been demonstrated to mediate this response4–7, and photoregulation of rbcS expression occurs at least in part at the level of transcription8,9. We have shown previously that a 5′-noncoding fragment (4–973 base pairs (bp) upstream of the messenger RNA cap site) of the pea rbcS ss3.6 gene contains all of the nucleotide sequence information necessary to direct the photoregulated expression of a bacterial chloramphenicol acetyltransferase (cat) gene in tobacco10. Consistent with these findings, Morelli et al. 11 have shown by deletion analysis of a second rbcS gene promoter, that the sequences required for photo-regulated expression of rbcS E9 reside within the 5′-noncoding region. They identified an upstream region of ~700 bp needed for maximum transcription but not light–dark regulation, and a region from −35 to −2 bp which included the TATA box and contained the necessary information for light responsiveness. We now demonstrate that regulatory sequences 5′ distal to the rbcS ss3.6 TATA box and transcriptional start site not only contain the information necessary for maximum expression, but also confer photoregulation. These upstream regulatory sequences function independently of orientation when fused to their homologous promoter or a heterologous promoter.

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MLA
Timko, Michael P., et al. “Light Regulation of Plant Gene Expression by an Upstream Enhancer-like Element.” NATURE, vol. 318, no. 6046, 1985, pp. 579–82, doi:10.1038/318579a0.
APA
Timko, M. P., Kausch, A. P., Castresana, C., Fassler, J., Herrera-Estrella, L., Van den Broeck, G., … Cashmore, A. R. (1985). Light regulation of plant gene expression by an upstream enhancer-like element. NATURE, 318(6046), 579–582. https://doi.org/10.1038/318579a0
Chicago author-date
Timko, Michael P, Albert P Kausch, Carmen Castresana, Jan Fassler, Luis Herrera-Estrella, Guido Van den Broeck, Marc Van Montagu, Jeff Schell, and Anthony R Cashmore. 1985. “Light Regulation of Plant Gene Expression by an Upstream Enhancer-like Element.” NATURE 318 (6046): 579–82. https://doi.org/10.1038/318579a0.
Chicago author-date (all authors)
Timko, Michael P, Albert P Kausch, Carmen Castresana, Jan Fassler, Luis Herrera-Estrella, Guido Van den Broeck, Marc Van Montagu, Jeff Schell, and Anthony R Cashmore. 1985. “Light Regulation of Plant Gene Expression by an Upstream Enhancer-like Element.” NATURE 318 (6046): 579–582. doi:10.1038/318579a0.
Vancouver
1.
Timko MP, Kausch AP, Castresana C, Fassler J, Herrera-Estrella L, Van den Broeck G, et al. Light regulation of plant gene expression by an upstream enhancer-like element. NATURE. 1985;318(6046):579–82.
IEEE
[1]
M. P. Timko et al., “Light regulation of plant gene expression by an upstream enhancer-like element,” NATURE, vol. 318, no. 6046, pp. 579–582, 1985.
@article{6974783,
  abstract     = {{Light regulates many varied physiological and developmental phenomena during plant growth and differentiation, including the formation of a photosynthetically competent chloroplast from a proplastid1. The expression of ribulose 1,5-bisphosphate carboxylase small subunit (rbcS) genes is regulated by light in a development- and tissue-specific manner2,3. In some plant species, phytochrome has been demonstrated to mediate this response4–7, and photoregulation of rbcS expression occurs at least in part at the level of transcription8,9. We have shown previously that a 5′-noncoding fragment (4–973 base pairs (bp) upstream of the messenger RNA cap site) of the pea rbcS ss3.6 gene contains all of the nucleotide sequence information necessary to direct the photoregulated expression of a bacterial chloramphenicol acetyltransferase (cat) gene in tobacco10. Consistent with these findings, Morelli et al. 11 have shown by deletion analysis of a second rbcS gene promoter, that the sequences required for photo-regulated expression of rbcS E9 reside within the 5′-noncoding region. They identified an upstream region of ~700 bp needed for maximum transcription but not light–dark regulation, and a region from −35 to −2 bp which included the TATA box and contained the necessary information for light responsiveness. We now demonstrate that regulatory sequences 5′ distal to the rbcS ss3.6 TATA box and transcriptional start site not only contain the information necessary for maximum expression, but also confer photoregulation. These upstream regulatory sequences function independently of orientation when fused to their homologous promoter or a heterologous promoter.}},
  author       = {{Timko, Michael P and Kausch, Albert P and Castresana, Carmen and Fassler, Jan and Herrera-Estrella, Luis and Van den Broeck, Guido and Van Montagu, Marc and Schell, Jeff and Cashmore, Anthony R}},
  issn         = {{0028-0836}},
  journal      = {{NATURE}},
  language     = {{eng}},
  number       = {{6046}},
  pages        = {{579--582}},
  title        = {{Light regulation of plant gene expression by an upstream enhancer-like element}},
  url          = {{http://doi.org/10.1038/318579a0}},
  volume       = {{318}},
  year         = {{1985}},
}

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