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Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer in plants

(1985) NUCLEIC ACIDS RESEARCH. 13(13). p.4777-4788
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Abstract
A two-component cloning system to transfer foreign DNA into plants was derived from the octopine Ti plasmid pTiB6S3. pGV2260 is a non-oncogenic Ti plasmid from which the T-region is deleted and substituted by pBR322. pGV831 is a streptomycin-resistant pBR325 derivative that contains a kanamycin resistance marker gene for plant cells and a site for cloning foreign genes between the 25-bp border sequences of the octopine T-region. Conjugative transfer of pGV831 derivatives to Agrobacterium and cointegration by homologous recombination between the pBR322 sequences present on pGV831 and pGV2260, can be obtained in a single step. Strains carrying the resulting cointegrated plasmids transfer and integrate T-DNA into the genome of tobacco protoplasts, and transformed tobacco calli are readily selected as resistant to kanamycin. Intact plants containing the entire DNA region between the T-DNA borders have been regenerated from such clones. In view of these properties we present pGV831 and its derivatives as vectors for efficient integration of foreign genes into plants.

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Chicago
Deblaere, Rolf, Benny Bytebier, Henri De Greve, Francine Deboeck, Jeff Schell, Marc Van Montagu, and Jan Leeman. 1985. “Efficient Octopine Ti Plasmid-derived Vectors for Agrobacterium-mediated Gene Transfer in Plants.” Nucleic Acids Research 13 (13): 4777–4788.
APA
Deblaere, R., Bytebier, B., De Greve, H., Deboeck, F., Schell, J., Van Montagu, M., & Leeman, J. (1985). Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer in plants. NUCLEIC ACIDS RESEARCH, 13(13), 4777–4788.
Vancouver
1.
Deblaere R, Bytebier B, De Greve H, Deboeck F, Schell J, Van Montagu M, et al. Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer in plants. NUCLEIC ACIDS RESEARCH. 1985;13(13):4777–88.
MLA
Deblaere, Rolf, Benny Bytebier, Henri De Greve, et al. “Efficient Octopine Ti Plasmid-derived Vectors for Agrobacterium-mediated Gene Transfer in Plants.” NUCLEIC ACIDS RESEARCH 13.13 (1985): 4777–4788. Print.
@article{6974774,
  abstract     = {A two-component cloning system to transfer foreign DNA into plants was derived from the octopine Ti plasmid pTiB6S3. pGV2260 is a non-oncogenic Ti plasmid from which the T-region is deleted and substituted by pBR322. pGV831 is a streptomycin-resistant pBR325 derivative that contains a kanamycin resistance marker gene for plant cells and a site for cloning foreign genes between the 25-bp border sequences of the octopine T-region. Conjugative transfer of pGV831 derivatives to Agrobacterium and cointegration by homologous recombination between the pBR322 sequences present on pGV831 and pGV2260, can be obtained in a single step. Strains carrying the resulting cointegrated plasmids transfer and integrate T-DNA into the genome of tobacco protoplasts, and transformed tobacco calli are readily selected as resistant to kanamycin. Intact plants containing the entire DNA region between the T-DNA borders have been regenerated from such clones. In view of these properties we present pGV831 and its derivatives as vectors for efficient integration of foreign genes into plants.},
  author       = {Deblaere, Rolf and Bytebier, Benny and De Greve, Henri and Deboeck, Francine and Schell, Jeff and Van Montagu, Marc and Leeman, Jan},
  issn         = {0305-1048},
  journal      = {NUCLEIC ACIDS RESEARCH},
  language     = {eng},
  number       = {13},
  pages        = {4777--4788},
  title        = {Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer in plants},
  url          = {http://dx.doi.org/10.1093/nar/13.13.4777},
  volume       = {13},
  year         = {1985},
}

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