Ghent University Academic Bibliography

Advanced

Hematopoietic plakophilin-3 regulates acute tissue-specific and systemic inflammation in mice

Tatyana Sklyarova UGent, Jolanda van Hengel UGent, Elien Van Wonterghem UGent, Claude Libert UGent, Frans Van Roy UGent and Roosmarijn Vandenbroucke UGent (2015) EUROPEAN JOURNAL OF IMMUNOLOGY. 45(10). p.2898-2910
abstract
Plakophilin-3 (PKP3) is a member of the armadillo protein family, which is important in cell-cell contacts and signaling during development and tumorigenesis. In conventional facilities, PKP3-deficient mice (PKP3(-/-)) develop spontaneous dermatitis, indicating a possible involvement of PKP3 in inflammatory responses. Here, we show that PKP3 deficiency sensitizes mice to irritant contact dermatitis induced by phorbol myristate acetate (PMA). This sensitization occurred in mice with PKP3 deficiency in the hematopoietic system (PKP3(-/-hem)), but not if the deficiency was specific to skin keratinocytes (PKP3(-/-ker)). In a model of dextran sulfate sodium induced colitis, ubiquitous PKP3 deletion, but not intestinal epithelial PKP3 deficiency (PKP3(-/-IEC)), impaired survival from disease. Interestingly, PKP3(-/-hem) mice also displayed increased sensitivity to dextran sulfate sodium induced colitis. Finally, PKP3(-/-) mice were more sensitive to the lethality of lipopolysaccharide (LPS) injection than wild-type (WT) mice, and this phenotype was associated with increased intestinal permeability. PKP3(-/-IEC) mice did not reproduce the enhanced endotoxin reactivity of PKP3(-/-) mice, in contrast to PKP3(-/-hem) mice. Finally, in vitro stimulation of WT neutrophils with LPS or PMA increased Pkp3 expression. In conclusion, our data highlight a novel role for hematopoietic PKP3 in the regulation of both locally and systemically induced immune responses. Nonetheless, further research is needed to unravel the underlying mechanism.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Dermatitis, Hematopoietic cell, Colitis, Acute inflammation, Intestine, Plakophilin-3, Sepsis, Skin, CRE-MEDIATED RECOMBINATION, RNA-BINDING PROTEINS, IN-VIVO, INTESTINAL INFLAMMATION, ATOPIC-DERMATITIS, EPITHELIAL-CELLS, BARRIER FUNCTION, TRAUMA PATIENTS, INJURY, GUT
journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
Eur. J. Immunol.
volume
45
issue
10
pages
2898 - 2910
Web of Science type
Article
Web of Science id
000362554800020
JCR category
IMMUNOLOGY
JCR impact factor
4.179 (2015)
JCR rank
42/150 (2015)
JCR quartile
2 (2015)
ISSN
0014-2980
DOI
10.1002/eji.201445440
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
6973767
handle
http://hdl.handle.net/1854/LU-6973767
date created
2015-11-03 16:56:45
date last changed
2016-12-19 15:41:36
@article{6973767,
  abstract     = {Plakophilin-3 (PKP3) is a member of the armadillo protein family, which is important in cell-cell contacts and signaling during development and tumorigenesis. In conventional facilities, PKP3-deficient mice (PKP3(-/-)) develop spontaneous dermatitis, indicating a possible involvement of PKP3 in inflammatory responses. Here, we show that PKP3 deficiency sensitizes mice to irritant contact dermatitis induced by phorbol myristate acetate (PMA). This sensitization occurred in mice with PKP3 deficiency in the hematopoietic system (PKP3(-/-hem)), but not if the deficiency was specific to skin keratinocytes (PKP3(-/-ker)). In a model of dextran sulfate sodium induced colitis, ubiquitous PKP3 deletion, but not intestinal epithelial PKP3 deficiency (PKP3(-/-IEC)), impaired survival from disease. Interestingly, PKP3(-/-hem) mice also displayed increased sensitivity to dextran sulfate sodium induced colitis. Finally, PKP3(-/-) mice were more sensitive to the lethality of lipopolysaccharide (LPS) injection than wild-type (WT) mice, and this phenotype was associated with increased intestinal permeability. PKP3(-/-IEC) mice did not reproduce the enhanced endotoxin reactivity of PKP3(-/-) mice, in contrast to PKP3(-/-hem) mice. Finally, in vitro stimulation of WT neutrophils with LPS or PMA increased Pkp3 expression. In conclusion, our data highlight a novel role for hematopoietic PKP3 in the regulation of both locally and systemically induced immune responses. Nonetheless, further research is needed to unravel the underlying mechanism.},
  author       = {Sklyarova, Tatyana and van Hengel, Jolanda and Van Wonterghem, Elien and Libert, Claude and Van Roy, Frans and Vandenbroucke, Roosmarijn},
  issn         = {0014-2980},
  journal      = {EUROPEAN JOURNAL OF IMMUNOLOGY},
  keyword      = {Dermatitis,Hematopoietic cell,Colitis,Acute inflammation,Intestine,Plakophilin-3,Sepsis,Skin,CRE-MEDIATED RECOMBINATION,RNA-BINDING PROTEINS,IN-VIVO,INTESTINAL INFLAMMATION,ATOPIC-DERMATITIS,EPITHELIAL-CELLS,BARRIER FUNCTION,TRAUMA PATIENTS,INJURY,GUT},
  language     = {eng},
  number       = {10},
  pages        = {2898--2910},
  title        = {Hematopoietic plakophilin-3 regulates acute tissue-specific and systemic inflammation in mice},
  url          = {http://dx.doi.org/10.1002/eji.201445440},
  volume       = {45},
  year         = {2015},
}

Chicago
Sklyarova, Tatyana, Jolanda van Hengel, Elien Van Wonterghem, Claude Libert, Frans Van Roy, and Roosmarijn Vandenbroucke. 2015. “Hematopoietic Plakophilin-3 Regulates Acute Tissue-specific and Systemic Inflammation in Mice.” European Journal of Immunology 45 (10): 2898–2910.
APA
Sklyarova, T., van Hengel, J., Van Wonterghem, E., Libert, C., Van Roy, F., & Vandenbroucke, R. (2015). Hematopoietic plakophilin-3 regulates acute tissue-specific and systemic inflammation in mice. EUROPEAN JOURNAL OF IMMUNOLOGY, 45(10), 2898–2910.
Vancouver
1.
Sklyarova T, van Hengel J, Van Wonterghem E, Libert C, Van Roy F, Vandenbroucke R. Hematopoietic plakophilin-3 regulates acute tissue-specific and systemic inflammation in mice. EUROPEAN JOURNAL OF IMMUNOLOGY. 2015;45(10):2898–910.
MLA
Sklyarova, Tatyana, Jolanda van Hengel, Elien Van Wonterghem, et al. “Hematopoietic Plakophilin-3 Regulates Acute Tissue-specific and Systemic Inflammation in Mice.” EUROPEAN JOURNAL OF IMMUNOLOGY 45.10 (2015): 2898–2910. Print.