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Development and characterization of a new human hepatic cell line

Eva Ramboer, Bram De Craene, Joey De Kock, Geert Berx UGent, Vera Rogiers, Tamara Vanhaecke and Mathieu Vinken (2015) EXCLI JOURNAL. 14. p.875-889
abstract
The increasing demand and hampered use of primary human hepatocytes for research purposes have urged scientists to search for alternative cell sources, such as immortalized hepatic cell lines. The aim of this study was to develop a human hepatic cell line using the combined overexpression of TERT and the cell cycle regulators cyclin D1 and mutant isoform CDK4R24C. Following transduction of adult human primary hepatocytes with the selected immortalization genes, cell growth was triggered and a cell line was established. When cultured under appropriate conditions, the cell line expressed several hepatocytic markers and liver-enriched transcription factors at the transcriptional and/or translational level, secreted liver-specific proteins and showed glycogen deposition. These results suggest that the immortalization strategy applied to primary human hepatocytes could generate a novel hepatic cell line that seems to retain some key hepatic characteristics.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
hepatic cell line, DNA-SYNTHESIS, immortalization, human hepatocytes, RAT HEPATOCYTES, EPITHELIAL-CELLS, IMMORTALIZED HUMAN HEPATOCYTES, LIVER-FAILURE, IN-VITRO, PROTEIN, ESTABLISHMENT, DEDIFFERENTIATION, RECOMBINATION
journal title
EXCLI JOURNAL
EXCLI J.
volume
14
pages
875 - 889
Web of Science type
Article
Web of Science id
000359202800023
JCR category
BIOLOGY
JCR impact factor
1.292 (2015)
JCR rank
46/86 (2015)
JCR quartile
3 (2015)
ISSN
1611-2156
DOI
10.17179/excli2015-424
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
6970694
handle
http://hdl.handle.net/1854/LU-6970694
date created
2015-10-29 15:56:19
date last changed
2017-05-12 11:59:22
@article{6970694,
  abstract     = {The increasing demand and hampered use of primary human hepatocytes for research purposes have urged scientists to search for alternative cell sources, such as immortalized hepatic cell lines. The aim of this study was to develop a human hepatic cell line using the combined overexpression of TERT and the cell cycle regulators cyclin D1 and mutant isoform CDK4R24C. Following transduction of adult human primary hepatocytes with the selected immortalization genes, cell growth was triggered and a cell line was established. When cultured under appropriate conditions, the cell line expressed several hepatocytic markers and liver-enriched transcription factors at the transcriptional and/or translational level, secreted liver-specific proteins and showed glycogen deposition. These results suggest that the immortalization strategy applied to primary human hepatocytes could generate a novel hepatic cell line that seems to retain some key hepatic characteristics.},
  author       = {Ramboer, Eva and De Craene, Bram and De Kock, Joey and Berx, Geert and Rogiers, Vera and Vanhaecke, Tamara and Vinken, Mathieu},
  issn         = {1611-2156},
  journal      = {EXCLI JOURNAL},
  keyword      = {hepatic cell line,DNA-SYNTHESIS,immortalization,human hepatocytes,RAT HEPATOCYTES,EPITHELIAL-CELLS,IMMORTALIZED HUMAN HEPATOCYTES,LIVER-FAILURE,IN-VITRO,PROTEIN,ESTABLISHMENT,DEDIFFERENTIATION,RECOMBINATION},
  language     = {eng},
  pages        = {875--889},
  title        = {Development and characterization of a new human hepatic cell line},
  url          = {http://dx.doi.org/10.17179/excli2015-424},
  volume       = {14},
  year         = {2015},
}

Chicago
Ramboer, Eva, Bram De Craene, Joey De Kock, Geert Berx, Vera Rogiers, Tamara Vanhaecke, and Mathieu Vinken. 2015. “Development and Characterization of a New Human Hepatic Cell Line.” Excli Journal 14: 875–889.
APA
Ramboer, E., De Craene, B., De Kock, J., Berx, G., Rogiers, V., Vanhaecke, T., & Vinken, M. (2015). Development and characterization of a new human hepatic cell line. EXCLI JOURNAL, 14, 875–889.
Vancouver
1.
Ramboer E, De Craene B, De Kock J, Berx G, Rogiers V, Vanhaecke T, et al. Development and characterization of a new human hepatic cell line. EXCLI JOURNAL. 2015;14:875–89.
MLA
Ramboer, Eva, Bram De Craene, Joey De Kock, et al. “Development and Characterization of a New Human Hepatic Cell Line.” EXCLI JOURNAL 14 (2015): 875–889. Print.