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Development and characterization of a new human hepatic cell line

(2015) EXCLI JOURNAL. 14. p.875-889
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Abstract
The increasing demand and hampered use of primary human hepatocytes for research purposes have urged scientists to search for alternative cell sources, such as immortalized hepatic cell lines. The aim of this study was to develop a human hepatic cell line using the combined overexpression of TERT and the cell cycle regulators cyclin D1 and mutant isoform CDK4R24C. Following transduction of adult human primary hepatocytes with the selected immortalization genes, cell growth was triggered and a cell line was established. When cultured under appropriate conditions, the cell line expressed several hepatocytic markers and liver-enriched transcription factors at the transcriptional and/or translational level, secreted liver-specific proteins and showed glycogen deposition. These results suggest that the immortalization strategy applied to primary human hepatocytes could generate a novel hepatic cell line that seems to retain some key hepatic characteristics.
Keywords
hepatic cell line, DNA-SYNTHESIS, immortalization, human hepatocytes, RAT HEPATOCYTES, EPITHELIAL-CELLS, IMMORTALIZED HUMAN HEPATOCYTES, LIVER-FAILURE, IN-VITRO, PROTEIN, ESTABLISHMENT, DEDIFFERENTIATION, RECOMBINATION

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Citation

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Chicago
Ramboer, Eva, Bram De Craene, Joey De Kock, Geert Berx, Vera Rogiers, Tamara Vanhaecke, and Mathieu Vinken. 2015. “Development and Characterization of a New Human Hepatic Cell Line.” Excli Journal 14: 875–889.
APA
Ramboer, E., De Craene, B., De Kock, J., Berx, G., Rogiers, V., Vanhaecke, T., & Vinken, M. (2015). Development and characterization of a new human hepatic cell line. EXCLI JOURNAL, 14, 875–889.
Vancouver
1.
Ramboer E, De Craene B, De Kock J, Berx G, Rogiers V, Vanhaecke T, et al. Development and characterization of a new human hepatic cell line. EXCLI JOURNAL. 2015;14:875–89.
MLA
Ramboer, Eva, Bram De Craene, Joey De Kock, et al. “Development and Characterization of a New Human Hepatic Cell Line.” EXCLI JOURNAL 14 (2015): 875–889. Print.
@article{6970694,
  abstract     = {The increasing demand and hampered use of primary human hepatocytes for research purposes have urged scientists to search for alternative cell sources, such as immortalized hepatic cell lines. The aim of this study was to develop a human hepatic cell line using the combined overexpression of TERT and the cell cycle regulators cyclin D1 and mutant isoform CDK4R24C. Following transduction of adult human primary hepatocytes with the selected immortalization genes, cell growth was triggered and a cell line was established. When cultured under appropriate conditions, the cell line expressed several hepatocytic markers and liver-enriched transcription factors at the transcriptional and/or translational level, secreted liver-specific proteins and showed glycogen deposition. These results suggest that the immortalization strategy applied to primary human hepatocytes could generate a novel hepatic cell line that seems to retain some key hepatic characteristics.},
  author       = {Ramboer, Eva and De Craene, Bram and De Kock, Joey and Berx, Geert and Rogiers, Vera and Vanhaecke, Tamara and Vinken, Mathieu},
  issn         = {1611-2156},
  journal      = {EXCLI JOURNAL},
  keyword      = {hepatic cell line,DNA-SYNTHESIS,immortalization,human hepatocytes,RAT HEPATOCYTES,EPITHELIAL-CELLS,IMMORTALIZED HUMAN HEPATOCYTES,LIVER-FAILURE,IN-VITRO,PROTEIN,ESTABLISHMENT,DEDIFFERENTIATION,RECOMBINATION},
  language     = {eng},
  pages        = {875--889},
  title        = {Development and characterization of a new human hepatic cell line},
  url          = {http://dx.doi.org/10.17179/excli2015-424},
  volume       = {14},
  year         = {2015},
}

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