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Effect of different adjuvants on protection and side-effects induced by Helicobacter suis whole-cell lysate vaccination

Iris Bosschem (UGent) , Jagadeesh Bayry, Ellen De Bruyne (UGent) , Kim Van Deun (UGent) , Annemieke Smet (UGent) , Griet Vercauteren (UGent) , Richard Ducatelle (UGent) , Freddy Haesebrouck (UGent) and Bram Flahou (UGent)
(2015) PLOS ONE. 10(6).
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Abstract
Helicobacter suis (H. suis) is a widespread porcine gastric pathogen, which is also of zoonotic importance. The first goal of this study was to investigate the efficacy of several vaccine adjuvants (CpG-DNA, Curdlan, Freund's Complete and Incomplete, Cholera toxin), administered either subcutaneously or intranasally along with H. suis whole-cell lysate, to protect against subsequent H. suis challenge in a BALB/c infection model. Subcutaneous immunization with Freund's complete (FC)/lysate and intranasal immunization with Cholera toxin (CT)/lysate were shown to be the best options for vaccination against H. suis, as determined by the amount of colonizing H. suis bacteria in the stomach, although adverse effects such as post-immunization gastritis/pseudo-pyloric metaplasia and increased mortality were observed, respectively. Therefore, we decided to test alternative strategies, including sublingual vaccine administration, to reduce the unwanted side-effects. A CCR4 antagonist that transiently inhibits the migration of regulatory T cells was also included as a new adjuvant in this second study. Results confirmed that immunization with CT (intranasally or sublingually) is among the most effective vaccination protocols, but increased mortality was still observed. In the groups immunized subcutaneously with FC/lysate and CCR4 antagonist/lysate, a significant protection was observed. Compared to the FC/lysate immunized group, gastric pseudo-pyloric metaplasia was less severe or even absent in the CCR4 antagonist/lysate immunized group. In general, an inverse correlation was observed between IFN-gamma, IL-4, IL-17, KC, MIP-2 and LIX mRNA expression and H. suis colonization density, whereas lower IL-10 expression levels were observed in partially protected animals.
Keywords
REGULATORY T-CELLS, PYLORI INFECTION, IMMUNE-RESPONSE, POSTIMMUNIZATION GASTRITIS, BACTERIAL-COLONIZATION, ORAL IMMUNIZATION, MOUSE MODEL, MICE, UREASE, CCR4

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Chicago
Bosschem, Iris, Jagadeesh Bayry, Ellen De Bruyne, Kim Van Deun, Annemieke Smet, Griet Vercauteren, Richard Ducatelle, Freddy Haesebrouck, and Bram Flahou. 2015. “Effect of Different Adjuvants on Protection and Side-effects Induced by Helicobacter Suis Whole-cell Lysate Vaccination.” Plos One 10 (6).
APA
Bosschem, I., Bayry, J., De Bruyne, E., Van Deun, K., Smet, A., Vercauteren, G., Ducatelle, R., et al. (2015). Effect of different adjuvants on protection and side-effects induced by Helicobacter suis whole-cell lysate vaccination. PLOS ONE, 10(6).
Vancouver
1.
Bosschem I, Bayry J, De Bruyne E, Van Deun K, Smet A, Vercauteren G, et al. Effect of different adjuvants on protection and side-effects induced by Helicobacter suis whole-cell lysate vaccination. PLOS ONE. 2015;10(6).
MLA
Bosschem, Iris, Jagadeesh Bayry, Ellen De Bruyne, et al. “Effect of Different Adjuvants on Protection and Side-effects Induced by Helicobacter Suis Whole-cell Lysate Vaccination.” PLOS ONE 10.6 (2015): n. pag. Print.
@article{6969010,
  abstract     = {Helicobacter suis (H. suis) is a widespread porcine gastric pathogen, which is also of zoonotic importance. The first goal of this study was to investigate the efficacy of several vaccine adjuvants (CpG-DNA, Curdlan, Freund's Complete and Incomplete, Cholera toxin), administered either subcutaneously or intranasally along with H. suis whole-cell lysate, to protect against subsequent H. suis challenge in a BALB/c infection model. Subcutaneous immunization with Freund's complete (FC)/lysate and intranasal immunization with Cholera toxin (CT)/lysate were shown to be the best options for vaccination against H. suis, as determined by the amount of colonizing H. suis bacteria in the stomach, although adverse effects such as post-immunization gastritis/pseudo-pyloric metaplasia and increased mortality were observed, respectively. Therefore, we decided to test alternative strategies, including sublingual vaccine administration, to reduce the unwanted side-effects. A CCR4 antagonist that transiently inhibits the migration of regulatory T cells was also included as a new adjuvant in this second study. Results confirmed that immunization with CT (intranasally or sublingually) is among the most effective vaccination protocols, but increased mortality was still observed. In the groups immunized subcutaneously with FC/lysate and CCR4 antagonist/lysate, a significant protection was observed. Compared to the FC/lysate immunized group, gastric pseudo-pyloric metaplasia was less severe or even absent in the CCR4 antagonist/lysate immunized group. In general, an inverse correlation was observed between IFN-gamma, IL-4, IL-17, KC, MIP-2 and LIX mRNA expression and H. suis colonization density, whereas lower IL-10 expression levels were observed in partially protected animals.},
  articleno    = {e0131364},
  author       = {Bosschem, Iris and Bayry, Jagadeesh and De Bruyne, Ellen and Van Deun, Kim and Smet, Annemieke and Vercauteren, Griet and Ducatelle, Richard and Haesebrouck, Freddy and Flahou, Bram},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {6},
  pages        = {19},
  title        = {Effect of different adjuvants on protection and side-effects induced by Helicobacter suis whole-cell lysate vaccination},
  url          = {http://dx.doi.org/10.1371/journal.pone.0131364},
  volume       = {10},
  year         = {2015},
}

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