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Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in vivo equine skin wound model

(2015) CYTOTHERAPY. 17(10). p.1434-1446
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Abstract
Background aims. Several studies report beneficial effects of autologous and allogeneic stem cells on wound healing. However, no comparison between autologous versus allogeneic epithelial-like stem cells (EpSCs) has been made so far. For this reason, we first hypothesize that both EpSC types enhance wound healing in comparison to vehicle treatment and untreated controls. Second, on the basis of other studies, we hypothesized that there would be no difference between autologous and allogeneic EpSCs. Methods. Twelve full-thickness skin wounds were created in six horses. Each horse was subjected to (i) autologous EpSCs, (ii) allogeneic EpSCs, (iii) vehicle treatment or (iv) untreated control. Wound evaluation was performed at day 3, 7 and 14 through wound exudates and at week 1, 2 and 5 through biopsies. Results. Wound circumference and surface were significantly smaller in autologous EpSC-treated wounds. A significantly lower amount of total granulation tissue (overall) and higher vascularization (week 1) was observed after both EpSC treatments. Significantly more major histocompatibility complex II-positive and CD20-positive cells were noticed in EpSC-treated wounds at week 2. In autologous and allogeneic groups, the number of EpSCs in center biopsies was low after 1 week (11.7% and 6.1%), decreased to 7.6% and 1.7%, respectively (week 2), and became undetectable at week 5. Conclusions. These results confirm the first hypothesis and partially support the second hypothesis. Besides macroscopic improvements, both autologous and allogeneic EpSCs had similar effects on granulation tissue formation, vascularization and early cellular immune response.
Keywords
autologous, horse, allogeneic, skin, stem cells, MESENCHYMAL STROMAL CELLS, DIGITAL FLEXOR TENDON, UMBILICAL-CORD BLOOD, BONE-MARROW, STEM/PROGENITOR CELLS, PERIPHERAL-BLOOD, IMMUNE-RESPONSE, LIP REPAIR, HORSES, VITRO

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Citation

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Chicago
Broeckx, Sarah Y, Bizunesh Mideksa Borena, Lore Van Hecke, Koen Chiers, Sofie Maes, Deborah J Guest, Evelyne Meyer, Luc Duchateau, Ann Martens, and Jan H Spaas. 2015. “Comparison of Autologous Versus Allogeneic Epithelial-like Stem Cell Treatment in an in Vivo Equine Skin Wound Model.” Cytotherapy 17 (10): 1434–1446.
APA
Broeckx, S. Y., Borena, B. M., Van Hecke, L., Chiers, K., Maes, S., Guest, D. J., Meyer, E., et al. (2015). Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in vivo equine skin wound model. CYTOTHERAPY, 17(10), 1434–1446.
Vancouver
1.
Broeckx SY, Borena BM, Van Hecke L, Chiers K, Maes S, Guest DJ, et al. Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in vivo equine skin wound model. CYTOTHERAPY. 2015;17(10):1434–46.
MLA
Broeckx, Sarah Y, Bizunesh Mideksa Borena, Lore Van Hecke, et al. “Comparison of Autologous Versus Allogeneic Epithelial-like Stem Cell Treatment in an in Vivo Equine Skin Wound Model.” CYTOTHERAPY 17.10 (2015): 1434–1446. Print.
@article{6968722,
  abstract     = {Background aims. Several studies report beneficial effects of autologous and allogeneic stem cells on wound healing. However, no comparison between autologous versus allogeneic epithelial-like stem cells (EpSCs) has been made so far. For this reason, we first hypothesize that both EpSC types enhance wound healing in comparison to vehicle treatment and untreated controls. Second, on the basis of other studies, we hypothesized that there would be no difference between autologous and allogeneic EpSCs.
Methods. Twelve full-thickness skin wounds were created in six horses. Each horse was subjected to (i) autologous EpSCs, (ii) allogeneic EpSCs, (iii) vehicle treatment or (iv) untreated control. Wound evaluation was performed at day 3, 7 and 14 through wound exudates and at week 1, 2 and 5 through biopsies.
Results. Wound circumference and surface were significantly smaller in autologous EpSC-treated wounds. A significantly lower amount of total granulation tissue (overall) and higher vascularization (week 1) was observed after both EpSC treatments. Significantly more major histocompatibility complex II-positive and CD20-positive cells were noticed in EpSC-treated wounds at week 2. In autologous and allogeneic groups, the number of EpSCs in center biopsies was low after 1 week (11.7\% and 6.1\%), decreased to 7.6\% and 1.7\%, respectively (week 2), and became undetectable at week 5.
Conclusions. These results confirm the first hypothesis and partially support the second hypothesis. Besides macroscopic improvements, both autologous and allogeneic EpSCs had similar effects on granulation tissue formation, vascularization and early cellular immune response.},
  author       = {Broeckx, Sarah Y and Borena, Bizunesh Mideksa and Van Hecke, Lore and Chiers, Koen and Maes, Sofie and Guest, Deborah J and Meyer, Evelyne and Duchateau, Luc and Martens, Ann and Spaas, Jan H},
  issn         = {1465-3249},
  journal      = {CYTOTHERAPY},
  language     = {eng},
  number       = {10},
  pages        = {1434--1446},
  title        = {Comparison of autologous versus allogeneic epithelial-like stem cell treatment in an in vivo equine skin wound model},
  url          = {http://dx.doi.org/10.1016/j.jcyt.2015.06.004},
  volume       = {17},
  year         = {2015},
}

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