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Inoculum selection influences the biochemical methane potential of agro-industrial substrates

(2015) MICROBIAL BIOTECHNOLOGY. 8(5). p.776-786
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Abstract
Obtaining a reliable estimation of the methane potential of organic waste streams in anaerobic digestion, for which a biochemical methane potential (BMP) test is often used, is of high importance. Standardization of this BMP test is required to ensure inter-laboratory repeatability and accuracy of the BMP results. Therefore, guidelines were set out; yet, these do not provide sufficient information concerning origin of and the microbial community in the test inoculum. Here, the specific contribution of the methanogenic community on the BMP test results was evaluated. The biomethane potential of four different substrates (molasses, bio-refinery waste, liquid manure and high-rate activated sludge) was determined by means of four different inocula from full-scale anaerobic digestion plants. A significant effect of the selected inoculum on the BMP result was observed for two out of four substrates. This inoculum effect could be attributed to the abundance of methanogens and a potential inhibiting effect in the inoculum itself, demonstrating the importance of inoculum selection for BMP testing. We recommend the application of granular sludge as an inoculum, because of its higher methanogenic abundance and activity, and protection from bulk solutions, compared with other inocula.
Keywords
MANURE, SLUDGE, CO-DIGESTION, MICROBIAL COMMUNITY, WASTE-WATER TREATMENT, ANAEROBIC-DIGESTION, INHIBITION, BIODEGRADABILITY, AMMONIA, BIOGAS

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Chicago
De Vrieze, Jo, Linde Raport, Bernard Willems, Silke Verbrugge, Eveline Volcke, Erik Meers, Largus T Angenent, and Nico Boon. 2015. “Inoculum Selection Influences the Biochemical Methane Potential of Agro-industrial Substrates.” Microbial Biotechnology 8 (5): 776–786.
APA
De Vrieze, Jo, Raport, L., Willems, B., Verbrugge, S., Volcke, E., Meers, E., Angenent, L. T., et al. (2015). Inoculum selection influences the biochemical methane potential of agro-industrial substrates. MICROBIAL BIOTECHNOLOGY, 8(5), 776–786.
Vancouver
1.
De Vrieze J, Raport L, Willems B, Verbrugge S, Volcke E, Meers E, et al. Inoculum selection influences the biochemical methane potential of agro-industrial substrates. MICROBIAL BIOTECHNOLOGY. 2015;8(5):776–86.
MLA
De Vrieze, Jo, Linde Raport, Bernard Willems, et al. “Inoculum Selection Influences the Biochemical Methane Potential of Agro-industrial Substrates.” MICROBIAL BIOTECHNOLOGY 8.5 (2015): 776–786. Print.
@article{6967545,
  abstract     = {Obtaining a reliable estimation of the methane potential of organic waste streams in anaerobic digestion, for which a biochemical methane potential (BMP) test is often used, is of high importance. Standardization of this BMP test is required to ensure inter-laboratory repeatability and accuracy of the BMP results. Therefore, guidelines were set out; yet, these do not provide sufficient information concerning origin of and the microbial community in the test inoculum. Here, the specific contribution of the methanogenic community on the BMP test results was evaluated. The biomethane potential of four different substrates (molasses, bio-refinery waste, liquid manure and high-rate activated sludge) was determined by means of four different inocula from full-scale anaerobic digestion plants. A significant effect of the selected inoculum on the BMP result was observed for two out of four substrates. This inoculum effect could be attributed to the abundance of methanogens and a potential inhibiting effect in the inoculum itself, demonstrating the importance of inoculum selection for BMP testing. We recommend the application of granular sludge as an inoculum, because of its higher methanogenic abundance and activity, and protection from bulk solutions, compared with other inocula.},
  author       = {De Vrieze, Jo and Raport, Linde and Willems, Bernard and Verbrugge, Silke and Volcke, Eveline and Meers, Erik and Angenent, Largus T and Boon, Nico},
  issn         = {1751-7907},
  journal      = {MICROBIAL BIOTECHNOLOGY},
  keyword      = {MANURE,SLUDGE,CO-DIGESTION,MICROBIAL COMMUNITY,WASTE-WATER TREATMENT,ANAEROBIC-DIGESTION,INHIBITION,BIODEGRADABILITY,AMMONIA,BIOGAS},
  language     = {eng},
  number       = {5},
  pages        = {776--786},
  title        = {Inoculum selection influences the biochemical methane potential of agro-industrial substrates},
  url          = {http://dx.doi.org/10.1111/1751-7915.12268},
  volume       = {8},
  year         = {2015},
}

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