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Functional genetic diversity analysis and identification of associated simple sequence repeats and amplified fragment length polymorphism markers to drought tolerance in lentil (Lens culinaris ssp. culinaris Medicus) landraces

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Abstract
Genetic diversity of 70 Mediterranean lentil (Lens culinaris ssp. culinaris Medicus) landraces was assessed using simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). These landraces were also assessed for variation in root and shoot traits and drought tolerance as estimated by relative water content (RWC), water losing rate (WLR) and wilting score (WS). Genetic diversity and clear differentiation of Moroccan landraces from those from northern Mediterranean regions (Italy, Turkey and Greece) were found. High genetic variation in root and shoot traits and traits related to drought tolerance was also observed. No relationship was found between drought tolerance of landraces and their geographic origin. Landraces with higher dry root biomass, chlorophyll content and root–shoot ratio were drought tolerant as evidenced by higher RWCand lower WLR and wilting severity. Kruskal–Wallis non-parametric test (KW) was used to find SSRs and AFLPs associated with RWC, WLR and WS. Regression analysis showed six SSR and AFLP alleles explaining the highest phenotypic variation of RWC, WLR and WS (ranging from 21 to 50 % for SSRs and from 14 to 33 % for AFLPs). Functional genetic diversity analysis showed relationships between drought response of landraces and linked SSR and AFLP alleles to RWC, WLR and WS according to K-W test using canonical discriminant analysis. Our results confirm the feasibility of using association mapping to find DNA markers associated with drought tolerance in larger numbers of lentil landraces.
Keywords
Stress ecophysiology, Marker–trait association, Drought, AFLP, SSR, Lentil landraces, Genetic diversity, CICER-ARIETINUM L., AVOIDANCE ROOT TRAITS, WATER DEFICITS, RESISTANCE, ADAPTATION, COLLECTION, ORIGINS, MEDIK., AFLP, POPULATIONS

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MLA
Idrissi, Omar et al. “Functional Genetic Diversity Analysis and Identification of Associated Simple Sequence Repeats and Amplified Fragment Length Polymorphism Markers to Drought Tolerance in Lentil (Lens Culinaris Ssp. Culinaris Medicus) Landraces.” PLANT MOLECULAR BIOLOGY REPORTER 34.3 (2016): 659–680. Print.
APA
Idrissi, O., Udupa, M. S., De Keyser, E., Van Damme, P., & De Riek, J. (2016). Functional genetic diversity analysis and identification of associated simple sequence repeats and amplified fragment length polymorphism markers to drought tolerance in lentil (Lens culinaris ssp. culinaris Medicus) landraces. PLANT MOLECULAR BIOLOGY REPORTER, 34(3), 659–680.
Chicago author-date
Idrissi, Omar, M Sripada Udupa, Ellen De Keyser, Patrick Van Damme, and Jan De Riek. 2016. “Functional Genetic Diversity Analysis and Identification of Associated Simple Sequence Repeats and Amplified Fragment Length Polymorphism Markers to Drought Tolerance in Lentil (Lens Culinaris Ssp. Culinaris Medicus) Landraces.” Plant Molecular Biology Reporter 34 (3): 659–680.
Chicago author-date (all authors)
Idrissi, Omar, M Sripada Udupa, Ellen De Keyser, Patrick Van Damme, and Jan De Riek. 2016. “Functional Genetic Diversity Analysis and Identification of Associated Simple Sequence Repeats and Amplified Fragment Length Polymorphism Markers to Drought Tolerance in Lentil (Lens Culinaris Ssp. Culinaris Medicus) Landraces.” Plant Molecular Biology Reporter 34 (3): 659–680.
Vancouver
1.
Idrissi O, Udupa MS, De Keyser E, Van Damme P, De Riek J. Functional genetic diversity analysis and identification of associated simple sequence repeats and amplified fragment length polymorphism markers to drought tolerance in lentil (Lens culinaris ssp. culinaris Medicus) landraces. PLANT MOLECULAR BIOLOGY REPORTER. 2016;34(3):659–80.
IEEE
[1]
O. Idrissi, M. S. Udupa, E. De Keyser, P. Van Damme, and J. De Riek, “Functional genetic diversity analysis and identification of associated simple sequence repeats and amplified fragment length polymorphism markers to drought tolerance in lentil (Lens culinaris ssp. culinaris Medicus) landraces,” PLANT MOLECULAR BIOLOGY REPORTER, vol. 34, no. 3, pp. 659–680, 2016.
@article{6965610,
  abstract     = {Genetic diversity of 70 Mediterranean lentil (Lens culinaris ssp. culinaris Medicus) landraces was assessed using simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). These landraces were also assessed for variation in root and shoot traits and drought tolerance as estimated by relative water content (RWC), water losing rate (WLR) and wilting score (WS). Genetic diversity and clear differentiation of Moroccan landraces from those from northern Mediterranean regions (Italy, Turkey and Greece) were found. High genetic variation in root and shoot traits and traits related to drought tolerance was also observed. No relationship was found between drought tolerance of landraces and their geographic origin. Landraces with higher dry root biomass, chlorophyll content and root–shoot ratio were drought tolerant as evidenced by higher RWCand lower WLR and wilting severity. Kruskal–Wallis non-parametric test (KW) was used to find SSRs and AFLPs associated with RWC, WLR and WS. Regression analysis showed six SSR and AFLP alleles explaining the highest phenotypic variation of RWC, WLR and WS (ranging from 21 to 50 % for SSRs and from 14 to 33 % for AFLPs). Functional genetic diversity analysis showed relationships between drought response of landraces and linked SSR and AFLP alleles to RWC, WLR and WS according to K-W test using canonical discriminant analysis. Our results confirm the feasibility of using association mapping to find DNA markers associated with drought tolerance in larger numbers of lentil landraces.},
  author       = {Idrissi, Omar and Udupa, M Sripada and De Keyser, Ellen and Van Damme, Patrick and De Riek, Jan},
  issn         = {0735-9640},
  journal      = {PLANT MOLECULAR BIOLOGY REPORTER},
  keywords     = {Stress ecophysiology,Marker–trait association,Drought,AFLP,SSR,Lentil landraces,Genetic diversity,CICER-ARIETINUM L.,AVOIDANCE ROOT TRAITS,WATER DEFICITS,RESISTANCE,ADAPTATION,COLLECTION,ORIGINS,MEDIK.,AFLP,POPULATIONS},
  language     = {eng},
  number       = {3},
  pages        = {659--680},
  title        = {Functional genetic diversity analysis and identification of associated simple sequence repeats and amplified fragment length polymorphism markers to drought tolerance in lentil (Lens culinaris ssp. culinaris Medicus) landraces},
  url          = {http://dx.doi.org/10.1007/s11105-015-0940-4},
  volume       = {34},
  year         = {2016},
}

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