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Genotypic diversity and differentiation among populations of two benthic freshwater diatoms as revealed by microsatellites

Pieter Vanormelingen, Katharine M Evans, David G Mann, Stacey Lance, Ann-Eline Debeer UGent, Sofie D'hondt UGent, Tine Verstraete UGent, Luc De Meester and Wim Vyverman UGent (2015) MOLECULAR ECOLOGY. 24(17). p.4433-4448
abstract
Given their large population sizes and presumed high dispersal capacity, protists are expected to exhibit homogeneous population structure over large spatial scales. On the other hand, the fragmented and short-lived nature of the lentic freshwater habitats that many protists inhabit promotes strong population differentiation. We used microsatellites in two benthic freshwater diatoms, Eunotia bilunaris robust' and Sellaphora capitata, sampled from within a pond and connected ponds, through isolated ponds from the same region to western Europe to determine the spatial scale at which differentiation appears. Because periods of low genotypic diversity contribute to population differentiation, we also assessed genotypic diversity. While genotypic diversity was very high to maximal in most samples of both species, some had a markedly lower diversity, with up to half (Eunotia) and over 90% (Sellaphora) of the strains having the same multilocus genotype. Population differentiation showed an isolation-by-distance pattern with very low standardized F-ST values between samples from the same or connected ponds but high values between isolated ponds, even when situated in the same region. Partial rbcL sequences in Eunotia were consistent with this pattern as isolated ponds in the same region could differ widely in haplotype composition. Populations identified by Structure corresponded to the source ponds, confirming that pond' is the main factor structuring these populations. We conclude that freshwater benthic diatom populations are highly fragmented on a regional scale, reflecting either less dispersal than is often assumed or reduced establishment success of immigrants, so that dispersal does not translate into gene flow.
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author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Eunotia bilunaris "robust', benthic diatoms, genotypic diversity, microsatellites, ponds, population differentiation, rbcL, Sellaphora capitata, MARINE PLANKTONIC DIATOM, SKELETONEMA-COSTATUM BACILLARIOPHYCEAE, DINOFLAGELLATE ALEXANDRIUM-TAMARENSE, NITZSCHIA-PUNGENS BACILLARIOPHYCEAE, SPECIES COMPLEX BACILLARIOPHYTA, GENETIC DIFFERENTIATION, DITYLUM-BRIGHTWELLII, NATURAL-POPULATIONS, COMPUTER-PROGRAM, COASTAL WATERS
journal title
MOLECULAR ECOLOGY
Mol. Ecol.
volume
24
issue
17
pages
4433 - 4448
Web of Science type
Article
Web of Science id
000360445700008
JCR category
ECOLOGY
JCR impact factor
5.947 (2015)
JCR rank
11/149 (2015)
JCR quartile
1 (2015)
ISSN
0962-1083
DOI
10.1111/mec.13336
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
6962408
handle
http://hdl.handle.net/1854/LU-6962408
date created
2015-10-16 15:25:12
date last changed
2016-12-19 15:45:27
@article{6962408,
  abstract     = {Given their large population sizes and presumed high dispersal capacity, protists are expected to exhibit homogeneous population structure over large spatial scales. On the other hand, the fragmented and short-lived nature of the lentic freshwater habitats that many protists inhabit promotes strong population differentiation. We used microsatellites in two benthic freshwater diatoms, Eunotia bilunaris robust' and Sellaphora capitata, sampled from within a pond and connected ponds, through isolated ponds from the same region to western Europe to determine the spatial scale at which differentiation appears. Because periods of low genotypic diversity contribute to population differentiation, we also assessed genotypic diversity. While genotypic diversity was very high to maximal in most samples of both species, some had a markedly lower diversity, with up to half (Eunotia) and over 90\% (Sellaphora) of the strains having the same multilocus genotype. Population differentiation showed an isolation-by-distance pattern with very low standardized F-ST values between samples from the same or connected ponds but high values between isolated ponds, even when situated in the same region. Partial rbcL sequences in Eunotia were consistent with this pattern as isolated ponds in the same region could differ widely in haplotype composition. Populations identified by Structure corresponded to the source ponds, confirming that pond' is the main factor structuring these populations. We conclude that freshwater benthic diatom populations are highly fragmented on a regional scale, reflecting either less dispersal than is often assumed or reduced establishment success of immigrants, so that dispersal does not translate into gene flow.},
  author       = {Vanormelingen, Pieter and Evans, Katharine M and Mann, David G and Lance, Stacey and Debeer, Ann-Eline and D'hondt, Sofie and Verstraete, Tine and De Meester, Luc and Vyverman, Wim},
  issn         = {0962-1083},
  journal      = {MOLECULAR ECOLOGY},
  keyword      = {Eunotia bilunaris {\textacutedbl}robust',benthic diatoms,genotypic diversity,microsatellites,ponds,population differentiation,rbcL,Sellaphora capitata,MARINE PLANKTONIC DIATOM,SKELETONEMA-COSTATUM BACILLARIOPHYCEAE,DINOFLAGELLATE ALEXANDRIUM-TAMARENSE,NITZSCHIA-PUNGENS BACILLARIOPHYCEAE,SPECIES COMPLEX BACILLARIOPHYTA,GENETIC DIFFERENTIATION,DITYLUM-BRIGHTWELLII,NATURAL-POPULATIONS,COMPUTER-PROGRAM,COASTAL WATERS},
  language     = {eng},
  number       = {17},
  pages        = {4433--4448},
  title        = {Genotypic diversity and differentiation among populations of two benthic freshwater diatoms as revealed by microsatellites},
  url          = {http://dx.doi.org/10.1111/mec.13336},
  volume       = {24},
  year         = {2015},
}

Chicago
Vanormelingen, Pieter, Katharine M Evans, David G Mann, Stacey Lance, Ann-Eline Debeer, Sofie D’hondt, Tine Verstraete, Luc De Meester, and Wim Vyverman. 2015. “Genotypic Diversity and Differentiation Among Populations of Two Benthic Freshwater Diatoms as Revealed by Microsatellites.” Molecular Ecology 24 (17): 4433–4448.
APA
Vanormelingen, P., Evans, K. M., Mann, D. G., Lance, S., Debeer, A.-E., D’hondt, S., Verstraete, T., et al. (2015). Genotypic diversity and differentiation among populations of two benthic freshwater diatoms as revealed by microsatellites. MOLECULAR ECOLOGY, 24(17), 4433–4448.
Vancouver
1.
Vanormelingen P, Evans KM, Mann DG, Lance S, Debeer A-E, D’hondt S, et al. Genotypic diversity and differentiation among populations of two benthic freshwater diatoms as revealed by microsatellites. MOLECULAR ECOLOGY. 2015;24(17):4433–48.
MLA
Vanormelingen, Pieter, Katharine M Evans, David G Mann, et al. “Genotypic Diversity and Differentiation Among Populations of Two Benthic Freshwater Diatoms as Revealed by Microsatellites.” MOLECULAR ECOLOGY 24.17 (2015): 4433–4448. Print.