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Eye extract improves cell migration out of lymphoid organ explants of L-vannamei and viability of the primary cell cultures

Wenfeng Li (UGent) , Vo Van Tuan (UGent) , Thuong Van Khuong (UGent) , Peter Bossier (UGent) and Hans Nauwynck (UGent)
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Abstract
Since no cell line from shrimp has been established up till now, an optimization of the primary cell culture protocol is necessary. In this context, the effect of extracts (supernatant of a 1:50 (w/v) suspension) from different shrimp organs (muscle, brain, ganglia, eyestalk, ovary, and eye) on the performance of primary lymphoid cell cultures was evaluated. Ten percent of eye extract and 3% of ovary extract enhanced maximally the migration and survival of cells of the lymphoid organ of Litopenaeus vannamei significantly at 48, 96, and 144 h post seeding. Extracts from the eyestalk (10%), muscle (10%), and brain (1%) significantly promoted the migration and survival of cells at 48 and 96 h post seeding but not anymore at 144 h post seeding. In conclusion, it may be advised to add 10% of eye extract or 3% of ovary extract to cells for the maximal health of primary cell cultures from the lymphoid organ of L. vannamei.
Keywords
L. vannamei, Lymphoid organ, Cell culture, Eye extract, Ovary extract, PENAEUS-MONODON FABRICIUS, SHRIMP, TISSUES, STYLIROSTRIS, MATURATION, SYSTEM, PRAWN, OVARY

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Citation

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Chicago
Li, Wenfeng, Vo Van Tuan, Thuong Van Khuong, Peter Bossier, and Hans Nauwynck. 2015. “Eye Extract Improves Cell Migration Out of Lymphoid Organ Explants of L-vannamei and Viability of the Primary Cell Cultures.” In Vitro Cellular & Developmental Biology-animal 51 (7): 651–654.
APA
Li, W., Tuan, V. V., Khuong, T. V., Bossier, P., & Nauwynck, H. (2015). Eye extract improves cell migration out of lymphoid organ explants of L-vannamei and viability of the primary cell cultures. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51(7), 651–654.
Vancouver
1.
Li W, Tuan VV, Khuong TV, Bossier P, Nauwynck H. Eye extract improves cell migration out of lymphoid organ explants of L-vannamei and viability of the primary cell cultures. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL. 2015;51(7):651–4.
MLA
Li, Wenfeng et al. “Eye Extract Improves Cell Migration Out of Lymphoid Organ Explants of L-vannamei and Viability of the Primary Cell Cultures.” IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 51.7 (2015): 651–654. Print.
@article{6957230,
  abstract     = {Since no cell line from shrimp has been established up till now, an optimization of the primary cell culture protocol is necessary. In this context, the effect of extracts (supernatant of a 1:50 (w/v) suspension) from different shrimp organs (muscle, brain, ganglia, eyestalk, ovary, and eye) on the performance of primary lymphoid cell cultures was evaluated. Ten percent of eye extract and 3% of ovary extract enhanced maximally the migration and survival of cells of the lymphoid organ of Litopenaeus vannamei significantly at 48, 96, and 144 h post seeding. Extracts from the eyestalk (10%), muscle (10%), and brain (1%) significantly promoted the migration and survival of cells at 48 and 96 h post seeding but not anymore at 144 h post seeding. In conclusion, it may be advised to add 10% of eye extract or 3% of ovary extract to cells for the maximal health of primary cell cultures from the lymphoid organ of L. vannamei.},
  author       = {Li, Wenfeng and Tuan, Vo Van and Khuong, Thuong Van and Bossier, Peter and Nauwynck, Hans},
  issn         = {1071-2690},
  journal      = {IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL},
  keywords     = {L. vannamei,Lymphoid organ,Cell culture,Eye extract,Ovary extract,PENAEUS-MONODON FABRICIUS,SHRIMP,TISSUES,STYLIROSTRIS,MATURATION,SYSTEM,PRAWN,OVARY},
  language     = {eng},
  number       = {7},
  pages        = {651--654},
  title        = {Eye extract improves cell migration out of lymphoid organ explants of L-vannamei and viability of the primary cell cultures},
  url          = {http://dx.doi.org/10.1007/s11626-015-9882-2},
  volume       = {51},
  year         = {2015},
}

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