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Wolbachia induces strong cytoplasmic incompatibility in the predatory bug Macrolophus pygmaeus

(2009) INSECT MOLECULAR BIOLOGY. 18(3). p.373-381
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Abstract
Macrolophus pygmaeus is a heteropteran predator that is widely used in European glasshouses for the biological control of whiteflies, aphids, thrips and spider mites. We have demonstrated that the insect is infected with the endosymbiotic bacterium Wolbachia pipientis. Several gene fragments of the endosymbiont were sequenced and subsequently used for phylogenetic analysis, revealing that it belongs to the Wolbachia supergroup B. The endosymbiont was visualized within the ovarioles using immunolocalization. Tetracycline treatments were used to cure M. pygmaeus from its infection. Although a completely cured line could not be obtained by this approach, the application of a constant antibiotic pressure over 13 generations resulted in a line with a significantly reduced Wolbachia concentration. Crosses performed with this tetracycline-treated line revealed that the endosymbiont causes severe cytoplasmic incompatibility. This is the first report of a reproductive effect induced by Wolbachia in an economically important heteropteran predator that may have vital implications for its commercial production and use in biological control.
Keywords
PHYLOGENETIC ANALYSIS, CALIGINOSUS HETEROPTERA, SEQUENCE TYPING SYSTEM, immunolocalization, BACTERIA, INFECTION, EVOLUTION, cytoplasmic incompatibility, Macrolophus pygmaeus, Wolbachia, STRAINS, MIRIDAE, DROSOPHILA, TRICHOGRAMMA

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Chicago
Machtelinckx, Thijs, Thomas Van Leeuwen, Bartel Vanholme, Bjorn Gehesquière, Wannes Dermauw, Bjorn Vandekerkhove, Godelieve Gheysen, and Patrick De Clercq. 2009. “Wolbachia Induces Strong Cytoplasmic Incompatibility in the Predatory Bug Macrolophus Pygmaeus.” Insect Molecular Biology 18 (3): 373–381.
APA
Machtelinckx, T., Van Leeuwen, T., Vanholme, B., Gehesquière, B., Dermauw, W., Vandekerkhove, B., Gheysen, G., et al. (2009). Wolbachia induces strong cytoplasmic incompatibility in the predatory bug Macrolophus pygmaeus. INSECT MOLECULAR BIOLOGY, 18(3), 373–381.
Vancouver
1.
Machtelinckx T, Van Leeuwen T, Vanholme B, Gehesquière B, Dermauw W, Vandekerkhove B, et al. Wolbachia induces strong cytoplasmic incompatibility in the predatory bug Macrolophus pygmaeus. INSECT MOLECULAR BIOLOGY. 2009;18(3):373–81.
MLA
Machtelinckx, Thijs, Thomas Van Leeuwen, Bartel Vanholme, et al. “Wolbachia Induces Strong Cytoplasmic Incompatibility in the Predatory Bug Macrolophus Pygmaeus.” INSECT MOLECULAR BIOLOGY 18.3 (2009): 373–381. Print.
@article{695267,
  abstract     = {Macrolophus pygmaeus is a heteropteran predator that is widely used in European glasshouses for the biological control of whiteflies, aphids, thrips and spider mites. We have demonstrated that the insect is infected with the endosymbiotic bacterium Wolbachia pipientis. Several gene fragments of the endosymbiont were sequenced and subsequently used for phylogenetic analysis, revealing that it belongs to the Wolbachia supergroup B. The endosymbiont was visualized within the ovarioles using immunolocalization. Tetracycline treatments were used to cure M. pygmaeus from its infection. Although a completely cured line could not be obtained by this approach, the application of a constant antibiotic pressure over 13 generations resulted in a line with a significantly reduced Wolbachia concentration. Crosses performed with this tetracycline-treated line revealed that the endosymbiont causes severe cytoplasmic incompatibility. This is the first report of a reproductive effect induced by Wolbachia in an economically important heteropteran predator that may have vital implications for its commercial production and use in biological control.},
  author       = {Machtelinckx, Thijs and Van Leeuwen, Thomas and Vanholme, Bartel and Gehesqui{\`e}re, Bjorn and Dermauw, Wannes and Vandekerkhove, Bjorn and Gheysen, Godelieve and De Clercq, Patrick},
  issn         = {0962-1075},
  journal      = {INSECT MOLECULAR BIOLOGY},
  keyword      = {PHYLOGENETIC ANALYSIS,CALIGINOSUS HETEROPTERA,SEQUENCE TYPING SYSTEM,immunolocalization,BACTERIA,INFECTION,EVOLUTION,cytoplasmic incompatibility,Macrolophus pygmaeus,Wolbachia,STRAINS,MIRIDAE,DROSOPHILA,TRICHOGRAMMA},
  language     = {eng},
  number       = {3},
  pages        = {373--381},
  title        = {Wolbachia induces strong cytoplasmic incompatibility in the predatory bug Macrolophus pygmaeus},
  url          = {http://dx.doi.org/10.1111/j.1365-2583.2009.00877.x},
  volume       = {18},
  year         = {2009},
}

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