Advanced search
1 file | 4.10 MB

Ethylene-mediated regulation of A2-type CYCLINs modulates hyponastic growth in Arabidopsis

(2015) PLANT PHYSIOLOGY. 169(1). p.194-208
Author
Organization
Abstract
Upward leaf movement (hyponastic growth) is frequently observed in response to changing environmental conditions and can be induced by the phytohormone ethylene. Hyponasty results from differential growth (i.e. enhanced cell elongation at the proximal abaxial side of the petiole relative to the adaxial side). Here, we characterize Enhanced Hyponasty-D, an activation-tagged Arabidopsis (Arabidopsis thaliana) line with exaggerated hyponasty. This phenotype is associated with overexpression of the mitotic cyclin CYCLINA2;1 (CYCA2;1), which hints at a role for cell divisions in regulating hyponasty. Indeed, mathematical analysis suggested that the observed changes in abaxial cell elongation rates during ethylene treatment should result in a larger hyponastic amplitude than observed, unless a decrease in cell proliferation rate at the proximal abaxial side of the petiole relative to the adaxial side was implemented. Our model predicts that when this differential proliferation mechanism is disrupted by either ectopic overexpression or mutation of CYCA2;1, the hyponastic growth response becomes exaggerated. This is in accordance with experimental observations on CYCA2;1 overexpression lines and cyca2;1 knockouts. We therefore propose a bipartite mechanism controlling leaf movement: ethylene induces longitudinal cell expansion in the abaxial petiole epidermis to induce hyponasty and simultaneously affects its amplitude by controlling cell proliferation through CYCA2;1. Further corroborating the model, we found that ethylene treatment results in transcriptional down-regulation of A2-type CYCLINs and propose that this, and possibly other regulatory mechanisms affecting CYCA2;1, may contribute to this attenuation of hyponastic growth.
Keywords
GENE-EXPRESSION, ROOT-GROWTH, HIGH-TEMPERATURE, SHADE-AVOIDANCE, TRANSCRIPTION FACTORS, CELL-DIVISION, DIFFERENTIAL PETIOLE GROWTH, A-TYPE CYCLIN, MICROTUBULE REORIENTATION, AUXIN TRANSPORT

Downloads

  • Polko et al. 2015 Plant Physiology 169 194.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 4.10 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Polko, Joanna K, Jop A van Rooij, Steffen Vanneste, Ronald Pierik, Ankie MH Ammerlaan, Marleen H Vergeer-van Eijk, Fionn McLoughlin, et al. 2015. “Ethylene-mediated Regulation of A2-type CYCLINs Modulates Hyponastic Growth in Arabidopsis.” Plant Physiology 169 (1): 194–208.
APA
Polko, J. K., van Rooij, J. A., Vanneste, S., Pierik, R., Ammerlaan, A. M., Vergeer-van Eijk, M. H., McLoughlin, F., et al. (2015). Ethylene-mediated regulation of A2-type CYCLINs modulates hyponastic growth in Arabidopsis. PLANT PHYSIOLOGY, 169(1), 194–208.
Vancouver
1.
Polko JK, van Rooij JA, Vanneste S, Pierik R, Ammerlaan AM, Vergeer-van Eijk MH, et al. Ethylene-mediated regulation of A2-type CYCLINs modulates hyponastic growth in Arabidopsis. PLANT PHYSIOLOGY. 2015;169(1):194–208.
MLA
Polko, Joanna K, Jop A van Rooij, Steffen Vanneste, et al. “Ethylene-mediated Regulation of A2-type CYCLINs Modulates Hyponastic Growth in Arabidopsis.” PLANT PHYSIOLOGY 169.1 (2015): 194–208. Print.
@article{6951449,
  abstract     = {Upward leaf movement (hyponastic growth) is frequently observed in response to changing environmental conditions and can be induced by the phytohormone ethylene. Hyponasty results from differential growth (i.e. enhanced cell elongation at the proximal abaxial side of the petiole relative to the adaxial side). Here, we characterize Enhanced Hyponasty-D, an activation-tagged Arabidopsis (Arabidopsis thaliana) line with exaggerated hyponasty. This phenotype is associated with overexpression of the mitotic cyclin CYCLINA2;1 (CYCA2;1), which hints at a role for cell divisions in regulating hyponasty. Indeed, mathematical analysis suggested that the observed changes in abaxial cell elongation rates during ethylene treatment should result in a larger hyponastic amplitude than observed, unless a decrease in cell proliferation rate at the proximal abaxial side of the petiole relative to the adaxial side was implemented. Our model predicts that when this differential proliferation mechanism is disrupted by either ectopic overexpression or mutation of CYCA2;1, the hyponastic growth response becomes exaggerated. This is in accordance with experimental observations on CYCA2;1 overexpression lines and cyca2;1 knockouts. We therefore propose a bipartite mechanism controlling leaf movement: ethylene induces longitudinal cell expansion in the abaxial petiole epidermis to induce hyponasty and simultaneously affects its amplitude by controlling cell proliferation through CYCA2;1. Further corroborating the model, we found that ethylene treatment results in transcriptional down-regulation of A2-type CYCLINs and propose that this, and possibly other regulatory mechanisms affecting CYCA2;1, may contribute to this attenuation of hyponastic growth.},
  author       = {Polko, Joanna K and van Rooij, Jop A and Vanneste, Steffen and Pierik, Ronald and Ammerlaan, Ankie MH and Vergeer-van Eijk, Marleen H and McLoughlin, Fionn and G{\"u}hl, Kerstin and Van Isterdael, Gert and Voesenek, Laurentius ACJ and Millenaar, Frank F and Beeckman, Tom and Peeters, Anton JM and Mar{\'e}e, Athanasius FM and van Zanten, Martijn},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {194--208},
  title        = {Ethylene-mediated regulation of A2-type CYCLINs modulates hyponastic growth in Arabidopsis},
  url          = {http://dx.doi.org/10.1104/pp.15.00343},
  volume       = {169},
  year         = {2015},
}

Altmetric
View in Altmetric
Web of Science
Times cited: