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RNA interference knockdown of BRASSINOSTEROID INSENSITIVE1 in maize reveals novel functions for brassinosteroid signaling in controlling plant architecture

(2015) PLANT PHYSIOLOGY. 169(1). p.826-839
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Brassinosteroids (BRs) are plant hormones involved in various growth and developmental processes. The BR signaling system is well established in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) but poorly understood in maize (Zea mays). BRASSINOSTEROID INSENSITIVE1 (BRI1) is a BR receptor, and database searches and additional genomic sequencing identified five maize homologs including duplicate copies of BRI1 itself. RNA interference (RNAi) using the extracellular coding region of a maize zmbril complementary DNA knocked down the expression of all five homologs. Decreased response to exogenously applied brassinolide and altered BR marker gene expression demonstrate that zmbriI-RNAi transgenic lines have compromised BR signaling. zmbriI-RNAi plants showed dwarf stature due to shortened internodes, with upper internodes most strongly affected. Leaves of zmbriI-RNAi plants are dark green, upright, and twisted, with decreased auricle formation. Kinematic analysis showed that decreased cell division and cell elongation both contributed to the shortened leaves. A BRASSINOSTEROID INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1-yellow fluorescent protein (BES1-YFP) transgenic line was developed that showed BR-inducible BES1-YFP accumulation in the nucleus, which was decreased in zmbriI-RNAi. Expression of the BES1-YFP reporter was strong in the auricle region of developing leaves, suggesting that localized BR signaling is involved in promoting auricle development, consistent with the zmbriI-RNAi phenotype. The blade-sheath boundary disruption, shorter ligule, and disrupted auricle morphology of RNAi lines resemble KNOTTED1-LIKE HOMEOBOX (KNOX) mutants, consistent with a mechanistic connection between KNOX genes and BR signaling.
Keywords
KNOX GENES, RICE, LEAF GROWTH, CELL-DIVISION, RECEPTOR KINASE BRI1, ARABIDOPSIS-THALIANA, REGULATORY NETWORK, GENE-EXPRESSION, GENOME-WIDE, GRAIN-YIELD

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Citation

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Chicago
Kir, Gokhan, Huaxun Ye, Hilde Nelissen, Anjanasree K Neelakandan, Andree S Kusnandar, Anding Luo, Dirk Inzé, Anne W Sylvester, Yanhai Yin, and Philip W Becraft. 2015. “RNA Interference Knockdown of BRASSINOSTEROID INSENSITIVE1 in Maize Reveals Novel Functions for Brassinosteroid Signaling in Controlling Plant Architecture.” Plant Physiology 169 (1): 826–839.
APA
Kir, G., Ye, H., Nelissen, H., Neelakandan, A. K., Kusnandar, A. S., Luo, A., Inzé, D., et al. (2015). RNA interference knockdown of BRASSINOSTEROID INSENSITIVE1 in maize reveals novel functions for brassinosteroid signaling in controlling plant architecture. PLANT PHYSIOLOGY, 169(1), 826–839.
Vancouver
1.
Kir G, Ye H, Nelissen H, Neelakandan AK, Kusnandar AS, Luo A, et al. RNA interference knockdown of BRASSINOSTEROID INSENSITIVE1 in maize reveals novel functions for brassinosteroid signaling in controlling plant architecture. PLANT PHYSIOLOGY. 2015;169(1):826–39.
MLA
Kir, Gokhan, Huaxun Ye, Hilde Nelissen, et al. “RNA Interference Knockdown of BRASSINOSTEROID INSENSITIVE1 in Maize Reveals Novel Functions for Brassinosteroid Signaling in Controlling Plant Architecture.” PLANT PHYSIOLOGY 169.1 (2015): 826–839. Print.
@article{6951401,
  abstract     = {Brassinosteroids (BRs) are plant hormones involved in various growth and developmental processes. The BR signaling system is well established in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) but poorly understood in maize (Zea mays). BRASSINOSTEROID INSENSITIVE1 (BRI1) is a BR receptor, and database searches and additional genomic sequencing identified five maize homologs including duplicate copies of BRI1 itself. RNA interference (RNAi) using the extracellular coding region of a maize zmbril complementary DNA knocked down the expression of all five homologs. Decreased response to exogenously applied brassinolide and altered BR marker gene expression demonstrate that zmbriI-RNAi transgenic lines have compromised BR signaling. zmbriI-RNAi plants showed dwarf stature due to shortened internodes, with upper internodes most strongly affected. Leaves of zmbriI-RNAi plants are dark green, upright, and twisted, with decreased auricle formation. Kinematic analysis showed that decreased cell division and cell elongation both contributed to the shortened leaves. A BRASSINOSTEROID INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1-yellow fluorescent protein (BES1-YFP) transgenic line was developed that showed BR-inducible BES1-YFP accumulation in the nucleus, which was decreased in zmbriI-RNAi. Expression of the BES1-YFP reporter was strong in the auricle region of developing leaves, suggesting that localized BR signaling is involved in promoting auricle development, consistent with the zmbriI-RNAi phenotype. The blade-sheath boundary disruption, shorter ligule, and disrupted auricle morphology of RNAi lines resemble KNOTTED1-LIKE HOMEOBOX (KNOX) mutants, consistent with a mechanistic connection between KNOX genes and BR signaling.},
  author       = {Kir, Gokhan and Ye, Huaxun and Nelissen, Hilde and Neelakandan, Anjanasree K and Kusnandar, Andree S and Luo, Anding and Inz{\'e}, Dirk and Sylvester, Anne W and Yin, Yanhai and Becraft, Philip W},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {KNOX GENES,RICE,LEAF GROWTH,CELL-DIVISION,RECEPTOR KINASE BRI1,ARABIDOPSIS-THALIANA,REGULATORY NETWORK,GENE-EXPRESSION,GENOME-WIDE,GRAIN-YIELD},
  language     = {eng},
  number       = {1},
  pages        = {826--839},
  title        = {RNA interference knockdown of BRASSINOSTEROID INSENSITIVE1 in maize reveals novel functions for brassinosteroid signaling in controlling plant architecture},
  url          = {http://dx.doi.org/10.1104/pp.15.00367},
  volume       = {169},
  year         = {2015},
}

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