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Myosin light chain phosphatase activation is involved in the hydrogen sulfide-induced relaxation in mouse gastric fundus

Ingeborg Dhaese UGent and Romain Lefebvre UGent (2009) European Journal of Pharmacology. 606(1-3). p.180-186
abstract
The relaxant effect of hydrogen sulfide (H2S) in the vascular tree is well established but its influence and mechanism of action in gastrointestinal smooth muscle was hardly investigated. The influence of H2S on contractility in mouse gastric fundus was therefore examined. Sodium hydrogen sulfide (NaHS; H2S donor) was administered to prostaglandin F2α (PGF2α)-contracted circular muscle strips of mouse gastric fundus, before and after incubation with interfering drugs. NaHS caused a concentration-dependent relaxation of the pre-contracted mouse gastric fundus strips. The K+ channels blockers glibenclamide, apamin, charybdotoxin, 4-aminopyridin and barium chloride had no influence on the NaHS-induced relaxation. The relaxation by NaHS was also not influenced by L-NAME, ODQ and SQ 22536, inhibitors of the cGMP and cAMP pathway, by nerve blockers capsazepine, ω-conotoxin and tetrodotoxin or by several channel and receptor blockers (ouabain, nifedipine, 2-aminoethyl diphenylborinate, ryanodine and thapsigargin). The myosin light chain phosphatase (MLCP) inhibitor calyculin-A reduced the NaHS-induced relaxation, but the Rho-kinase inhibitor Y-27632 had no influence.We showthat NaHS is able to relax PGF2α-contractedmouse gastric fundus strips. The results suggest that in the mouse gastric fundus, H2S causes relaxation at least partially via activation of MLCP.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Hydrogen sulfide, Sodium hydrogen sulfide, Calcium desensitization, Myosin light chain phosphatase, Rho-kinase, Calyculin-A, Y-27632, Mouse gastric fundus
journal title
European Journal of Pharmacology
Eur. J. Pharmacol.
volume
606
issue
1-3
pages
180 - 186
publisher
Elsevier
Web of Science type
Article
Web of Science id
000264988900026
JCR category
PHARMACOLOGY & PHARMACY
JCR impact factor
2.585 (2009)
JCR rank
102/236 (2009)
JCR quartile
2 (2009)
ISSN
0014-2999
DOI
10.1016/j.ephar.2009.01.011
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
694382
handle
http://hdl.handle.net/1854/LU-694382
date created
2009-06-12 15:13:19
date last changed
2009-06-23 10:13:12
@article{694382,
  abstract     = {The relaxant effect of hydrogen sulfide (H2S) in the vascular tree is well established but its influence and
mechanism of action in gastrointestinal smooth muscle was hardly investigated. The influence of H2S on
contractility in mouse gastric fundus was therefore examined. Sodium hydrogen sulfide (NaHS; H2S donor)
was administered to prostaglandin F2\ensuremath{\alpha} (PGF2\ensuremath{\alpha})-contracted circular muscle strips of mouse gastric fundus,
before and after incubation with interfering drugs. NaHS caused a concentration-dependent relaxation of the
pre-contracted mouse gastric fundus strips. The K+ channels blockers glibenclamide, apamin, charybdotoxin,
4-aminopyridin and barium chloride had no influence on the NaHS-induced relaxation. The relaxation by
NaHS was also not influenced by L-NAME, ODQ and SQ 22536, inhibitors of the cGMP and cAMP pathway, by
nerve blockers capsazepine, \ensuremath{\omega}-conotoxin and tetrodotoxin or by several channel and receptor blockers
(ouabain, nifedipine, 2-aminoethyl diphenylborinate, ryanodine and thapsigargin). The myosin light chain
phosphatase (MLCP) inhibitor calyculin-A reduced the NaHS-induced relaxation, but the Rho-kinase inhibitor
Y-27632 had no influence.We showthat NaHS is able to relax PGF2\ensuremath{\alpha}-contractedmouse gastric fundus strips. The
results suggest that in the mouse gastric fundus, H2S causes relaxation at least partially via activation of MLCP.},
  author       = {Dhaese, Ingeborg and Lefebvre, Romain},
  issn         = {0014-2999},
  journal      = {European Journal of Pharmacology},
  keyword      = {Hydrogen sulfide,Sodium hydrogen sulfide,Calcium desensitization,Myosin light chain phosphatase,Rho-kinase,Calyculin-A,Y-27632,Mouse gastric fundus},
  language     = {eng},
  number       = {1-3},
  pages        = {180--186},
  publisher    = {Elsevier},
  title        = {Myosin light chain phosphatase activation is involved in the hydrogen sulfide-induced relaxation in mouse gastric fundus},
  url          = {http://dx.doi.org/10.1016/j.ephar.2009.01.011},
  volume       = {606},
  year         = {2009},
}

Chicago
Dhaese, Ingeborg, and Romain Lefebvre. 2009. “Myosin Light Chain Phosphatase Activation Is Involved in the Hydrogen Sulfide-induced Relaxation in Mouse Gastric Fundus.” European Journal of Pharmacology 606 (1-3): 180–186.
APA
Dhaese, I., & Lefebvre, R. (2009). Myosin light chain phosphatase activation is involved in the hydrogen sulfide-induced relaxation in mouse gastric fundus. European Journal of Pharmacology, 606(1-3), 180–186.
Vancouver
1.
Dhaese I, Lefebvre R. Myosin light chain phosphatase activation is involved in the hydrogen sulfide-induced relaxation in mouse gastric fundus. European Journal of Pharmacology. Elsevier; 2009;606(1-3):180–6.
MLA
Dhaese, Ingeborg, and Romain Lefebvre. “Myosin Light Chain Phosphatase Activation Is Involved in the Hydrogen Sulfide-induced Relaxation in Mouse Gastric Fundus.” European Journal of Pharmacology 606.1-3 (2009): 180–186. Print.