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Cyclodextrin-peptide conjugates for sequence specific DNA binding

(2015) ORGANIC & BIOMOLECULAR CHEMISTRY. 13(18). p.5273-5278
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Abstract
Synthetic models of bZIP transcription factors have been developed with the capability of specific DNA recognition. Our design is based on the CuAAC mediated conjugation of basic region Leucine Zipper peptides to different derivatives of alpha,beta and gamma-cyclodextrins equipped with azide functionalities. Thorough optimization of reaction conditions allowed convergent and simultaneous conjugation of two long unprotected cationic peptides to cyclodextrin-bis azide derivatives. The resulting constructs were shown to specifically recognize their cognate DNA sequence with nM affinities. In comparison with previously developed TF models, the derivatives described here combine the enhanced DNA binding capabilities with an easy and convergent synthetic route.
Keywords
DIMER, DISCOVERY, GENE, RECOGNITION, CLICK CHEMISTRY, INCLUSION COMPLEX, CRYSTAL-STRUCTURE, THERAPY, TARGETS, CELLS

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Citation

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MLA
Ruiz Garcia, Yara et al. “Cyclodextrin-peptide Conjugates for Sequence Specific DNA Binding.” ORGANIC & BIOMOLECULAR CHEMISTRY 13.18 (2015): 5273–5278. Print.
APA
Ruiz Garcia, Y., Zelenka, J., Pabon, Y. V., Iyer, A., Buděšínský, M., Kraus, T., Smith, C. E., et al. (2015). Cyclodextrin-peptide conjugates for sequence specific DNA binding. ORGANIC & BIOMOLECULAR CHEMISTRY, 13(18), 5273–5278.
Chicago author-date
Ruiz Garcia, Yara, Jan Zelenka, Y Vladimir Pabon, Abhishek Iyer, Miloš Buděšínský, Tomáš Kraus, CI Edvard Smith, and Annemieke Madder. 2015. “Cyclodextrin-peptide Conjugates for Sequence Specific DNA Binding.” Organic & Biomolecular Chemistry 13 (18): 5273–5278.
Chicago author-date (all authors)
Ruiz Garcia, Yara, Jan Zelenka, Y Vladimir Pabon, Abhishek Iyer, Miloš Buděšínský, Tomáš Kraus, CI Edvard Smith, and Annemieke Madder. 2015. “Cyclodextrin-peptide Conjugates for Sequence Specific DNA Binding.” Organic & Biomolecular Chemistry 13 (18): 5273–5278.
Vancouver
1.
Ruiz Garcia Y, Zelenka J, Pabon YV, Iyer A, Buděšínský M, Kraus T, et al. Cyclodextrin-peptide conjugates for sequence specific DNA binding. ORGANIC & BIOMOLECULAR CHEMISTRY. 2015;13(18):5273–8.
IEEE
[1]
Y. Ruiz Garcia et al., “Cyclodextrin-peptide conjugates for sequence specific DNA binding,” ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 13, no. 18, pp. 5273–5278, 2015.
@article{6931351,
  abstract     = {Synthetic models of bZIP transcription factors have been developed with the capability of specific DNA recognition. Our design is based on the CuAAC mediated conjugation of basic region Leucine Zipper peptides to different derivatives of alpha,beta and gamma-cyclodextrins equipped with azide functionalities. Thorough optimization of reaction conditions allowed convergent and simultaneous conjugation of two long unprotected cationic peptides to cyclodextrin-bis azide derivatives. The resulting constructs were shown to specifically recognize their cognate DNA sequence with nM affinities. In comparison with previously developed TF models, the derivatives described here combine the enhanced DNA binding capabilities with an easy and convergent synthetic route.},
  author       = {Ruiz Garcia, Yara and Zelenka, Jan and Pabon, Y Vladimir and Iyer, Abhishek and Buděšínský, Miloš and Kraus, Tomáš and Smith, CI Edvard and Madder, Annemieke},
  issn         = {1477-0520},
  journal      = {ORGANIC & BIOMOLECULAR CHEMISTRY},
  keywords     = {DIMER,DISCOVERY,GENE,RECOGNITION,CLICK CHEMISTRY,INCLUSION COMPLEX,CRYSTAL-STRUCTURE,THERAPY,TARGETS,CELLS},
  language     = {eng},
  number       = {18},
  pages        = {5273--5278},
  title        = {Cyclodextrin-peptide conjugates for sequence specific DNA binding},
  url          = {http://dx.doi.org/10.1039/c5ob00609k},
  volume       = {13},
  year         = {2015},
}

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