Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
Add to list
  1. Search results
  2. Next generation sequencing shows West...

Next generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model

Maha Dridi, Toon Rosseel (UGent) , Richard Orton, Paul Johnson, Sylvie Lecollinet, Benoît Muylkens, Bénédicte Lambrecht and Steven Van Borm
(2015) JOURNAL OF GENERAL VIROLOGY. 96(10). p.2999-3009
Author
Organization
Abstract
West Nile virus (WNV) occurs as a population of genetic variants (quasispecies) infecting a single animal. Previous low resolution viral genetic diversity estimates in sampled wild birds and mosquitoes and in multiple passage adaptation studies in vivo or in cell culture, suggest that WNV genetic diversification is mostly limited to the mosquito vector. This study investigates genetic diversification of WNV in avian hosts during a single passage using next generation sequencing. Wild-captured Carrion crows were subcutaneously infected using a clonal Middle-East WNV. Blood samples were collected on 2 and 4 days post-infection. A RT-PCR approach was used to amplify the WNV genome directly from serum samples prior to next generation sequencing resulting in an average depth of at least 700x in each sample. Appropriate controls were sequenced to discriminate biologically relevant low frequency variants from experimentally introduced errors. The WNV populations in the wild crows showed significant diversification away from the inoculum virus quasispecies structure. By contrast, WNV populations in intracerebrally infected day-old chickens did not diversify from that of the inoculum. Where previous studies concluded that WNV genetic diversification is only experimentally demonstrated in its permissive insect vector species, we have experimentally shown significant diversification of WNV populations in a wild bird reservoir species.
Keywords
PURIFYING SELECTION, UNTRANSLATED REGION, SECONDARY STRUCTURE, GENETIC DIVERSITY, RNA REPLICATION, PATHOGENESIS, MOSQUITOS, EVOLUTION, GENOME, BIRDS

Citation

Please use this url to cite or link to this publication:

MLA
Dridi, Maha, et al. “Next Generation Sequencing Shows West Nile Virus Quasispecies Diversification after a Single Passage in a Carrion Crow (Corvus Corone) in Vivo Infection Model.” JOURNAL OF GENERAL VIROLOGY, vol. 96, no. 10, 2015, pp. 2999–3009, doi:10.1099/jgv.0.000231.
APA
Dridi, M., Rosseel, T., Orton, R., Johnson, P., Lecollinet, S., Muylkens, B., … Van Borm, S. (2015). Next generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model. JOURNAL OF GENERAL VIROLOGY, 96(10), 2999–3009. https://doi.org/10.1099/jgv.0.000231
Chicago author-date
Dridi, Maha, Toon Rosseel, Richard Orton, Paul Johnson, Sylvie Lecollinet, Benoît Muylkens, Bénédicte Lambrecht, and Steven Van Borm. 2015. “Next Generation Sequencing Shows West Nile Virus Quasispecies Diversification after a Single Passage in a Carrion Crow (Corvus Corone) in Vivo Infection Model.” JOURNAL OF GENERAL VIROLOGY 96 (10): 2999–3009. https://doi.org/10.1099/jgv.0.000231.
Chicago author-date (all authors)
Dridi, Maha, Toon Rosseel, Richard Orton, Paul Johnson, Sylvie Lecollinet, Benoît Muylkens, Bénédicte Lambrecht, and Steven Van Borm. 2015. “Next Generation Sequencing Shows West Nile Virus Quasispecies Diversification after a Single Passage in a Carrion Crow (Corvus Corone) in Vivo Infection Model.” JOURNAL OF GENERAL VIROLOGY 96 (10): 2999–3009. doi:10.1099/jgv.0.000231.
Vancouver
1.
Dridi M, Rosseel T, Orton R, Johnson P, Lecollinet S, Muylkens B, et al. Next generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model. JOURNAL OF GENERAL VIROLOGY. 2015;96(10):2999–3009.
IEEE
[1]
M. Dridi et al., “Next generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model,” JOURNAL OF GENERAL VIROLOGY, vol. 96, no. 10, pp. 2999–3009, 2015.
@article{6931323,
  abstract     = {{West Nile virus (WNV) occurs as a population of genetic variants (quasispecies) infecting a single animal. Previous low resolution viral genetic diversity estimates in sampled wild birds and mosquitoes and in multiple passage adaptation studies in vivo or in cell culture, suggest that WNV genetic diversification is mostly limited to the mosquito vector. This study investigates genetic diversification of WNV in avian hosts during a single passage using next generation sequencing. Wild-captured Carrion crows were subcutaneously infected using a clonal Middle-East WNV. Blood samples were collected on 2 and 4 days post-infection. A RT-PCR approach was used to amplify the WNV genome directly from serum samples prior to next generation sequencing resulting in an average depth of at least 700x in each sample. Appropriate controls were sequenced to discriminate biologically relevant low frequency variants from experimentally introduced errors. The WNV populations in the wild crows showed significant diversification away from the inoculum virus quasispecies structure. By contrast, WNV populations in intracerebrally infected day-old chickens did not diversify from that of the inoculum. Where previous studies concluded that WNV genetic diversification is only experimentally demonstrated in its permissive insect vector species, we have experimentally shown significant diversification of WNV populations in a wild bird reservoir species.}},
  author       = {{Dridi, Maha and Rosseel, Toon and Orton, Richard and Johnson, Paul and Lecollinet, Sylvie and Muylkens, Benoît and Lambrecht, Bénédicte and Van Borm, Steven}},
  issn         = {{0022-1317}},
  journal      = {{JOURNAL OF GENERAL VIROLOGY}},
  keywords     = {{PURIFYING SELECTION,UNTRANSLATED REGION,SECONDARY STRUCTURE,GENETIC DIVERSITY,RNA REPLICATION,PATHOGENESIS,MOSQUITOS,EVOLUTION,GENOME,BIRDS}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{2999--3009}},
  title        = {{Next generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model}},
  url          = {{http://doi.org/10.1099/jgv.0.000231}},
  volume       = {{96}},
  year         = {{2015}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: