Advanced search
1 file | 3.56 MB Add to list

Genetic characterization of the Belgian nephropathogenic infectious bronchitis virus (NIBV) reference strain B1648

(2015) VIRUSES-BASEL. 7(8). p.4488-4506
Author
Organization
Abstract
The virulent nephropathogenic infectious bronchitis virus (NIBV) strain B1648 was first isolated in 1984, in Flanders, Belgium. Despite intensive vaccination, B1648 and its variants are still circulating in Europe and North Africa. Here, the full-length genome of this Belgian NIBV reference strain was determined by next generation sequencing (NGS) to understand its evolutionary relationship with other IBV strains, and to identify possible genetic factors that may be associated with the nephropathogenicity. Thirteen open reading frames (ORFs) were predicted in the B1648 strain (51UTR-1a-1b-S-3a-3b-E-M-4b-4c-5a-5b-N-6b-31UTR). ORFs 4b, 4c and 6b, which have been rarely reported in literature, were present in B1648 and most of the other IBV complete genomes. According to phylogenetic analysis of the full-length genome, replicase transcriptase complex, spike protein, partial S1 gene and M protein, B1648 strain clustered with the non-Massachusetts type strains NGA/A116E7/2006, UKr 27-11, QX-like ITA/90254/2005, QX-like CK/SWE/0658946/10, TN20/00, RF-27/99, RF/06/2007 and SLO/266/05. Based on the partial S1 fragment, B1648 clustered with the strains TN20/00, RF-27/99, RF/06/2007 and SLO/266/05 and, further designated as B1648 genotype. The full-length genome of B1648 shared the highest sequence homology with UKr 27-11, Gray, JMK, and NGA/A116E7/2006 (91.2% to 91.6%) and was least related with the reference Beaudette and Massachusetts strains (89.7%). Nucleotide and amino acid sequence analyses indicated that B1648 strain may have played an important role in the evolution of IBV in Europe and North Africa. Further, the nephropathogenicity determinants might be located on the 1a, spike, M and accessory proteins (3a, 3b, 4b, 4c, 5a, 5b and 6b). Overall, strain B1648 is distinct from all the strains reported so far in Europe and other parts of the world.
Keywords
B1648, IBV, nephropathogenic, chicken, nonstructural protein, MOUSE HEPATITIS-VIRUS, CORONAVIRUS MERS-COV, TURKEY CORONAVIRUS, CHICKENS, PATHOGENICITY, PROTECTION, SEQUENCE, IDENTIFICATION, EMERGENCE, GENOTYPES

Downloads

  • Genetic Characterization of the Belgian Nephropathogenic Infectious Bronchitis Virus NIBV Reference Strain B1648.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 3.56 MB

Citation

Please use this url to cite or link to this publication:

MLA
Avalakuppa Papi Reddy, Vishwanatha Reddy et al. “Genetic Characterization of the Belgian Nephropathogenic Infectious Bronchitis Virus (NIBV) Reference Strain B1648.” VIRUSES-BASEL 7.8 (2015): 4488–4506. Print.
APA
Avalakuppa Papi Reddy, V. R., Theuns, S., Roukaerts, I., Zeller, M., Matthijnssens, J., & Nauwynck, H. (2015). Genetic characterization of the Belgian nephropathogenic infectious bronchitis virus (NIBV) reference strain B1648. VIRUSES-BASEL, 7(8), 4488–4506.
Chicago author-date
Avalakuppa Papi Reddy, Vishwanatha Reddy, Sebastiaan Theuns, Inge Roukaerts, Mark Zeller, Jelle Matthijnssens, and Hans Nauwynck. 2015. “Genetic Characterization of the Belgian Nephropathogenic Infectious Bronchitis Virus (NIBV) Reference Strain B1648.” Viruses-basel 7 (8): 4488–4506.
Chicago author-date (all authors)
Avalakuppa Papi Reddy, Vishwanatha Reddy, Sebastiaan Theuns, Inge Roukaerts, Mark Zeller, Jelle Matthijnssens, and Hans Nauwynck. 2015. “Genetic Characterization of the Belgian Nephropathogenic Infectious Bronchitis Virus (NIBV) Reference Strain B1648.” Viruses-basel 7 (8): 4488–4506.
Vancouver
1.
Avalakuppa Papi Reddy VR, Theuns S, Roukaerts I, Zeller M, Matthijnssens J, Nauwynck H. Genetic characterization of the Belgian nephropathogenic infectious bronchitis virus (NIBV) reference strain B1648. VIRUSES-BASEL. 2015;7(8):4488–506.
IEEE
[1]
V. R. Avalakuppa Papi Reddy, S. Theuns, I. Roukaerts, M. Zeller, J. Matthijnssens, and H. Nauwynck, “Genetic characterization of the Belgian nephropathogenic infectious bronchitis virus (NIBV) reference strain B1648,” VIRUSES-BASEL, vol. 7, no. 8, pp. 4488–4506, 2015.
@article{6930954,
  abstract     = {The virulent nephropathogenic infectious bronchitis virus (NIBV) strain B1648 was first isolated in 1984, in Flanders, Belgium. Despite intensive vaccination, B1648 and its variants are still circulating in Europe and North Africa. Here, the full-length genome of this Belgian NIBV reference strain was determined by next generation sequencing (NGS) to understand its evolutionary relationship with other IBV strains, and to identify possible genetic factors that may be associated with the nephropathogenicity. Thirteen open reading frames (ORFs) were predicted in the B1648 strain (51UTR-1a-1b-S-3a-3b-E-M-4b-4c-5a-5b-N-6b-31UTR). ORFs 4b, 4c and 6b, which have been rarely reported in literature, were present in B1648 and most of the other IBV complete genomes. According to phylogenetic analysis of the full-length genome, replicase transcriptase complex, spike protein, partial S1 gene and M protein, B1648 strain clustered with the non-Massachusetts type strains NGA/A116E7/2006, UKr 27-11, QX-like ITA/90254/2005, QX-like CK/SWE/0658946/10, TN20/00, RF-27/99, RF/06/2007 and SLO/266/05. Based on the partial S1 fragment, B1648 clustered with the strains TN20/00, RF-27/99, RF/06/2007 and SLO/266/05 and, further designated as B1648 genotype. The full-length genome of B1648 shared the highest sequence homology with UKr 27-11, Gray, JMK, and NGA/A116E7/2006 (91.2% to 91.6%) and was least related with the reference Beaudette and Massachusetts strains (89.7%). Nucleotide and amino acid sequence analyses indicated that B1648 strain may have played an important role in the evolution of IBV in Europe and North Africa. Further, the nephropathogenicity determinants might be located on the 1a, spike, M and accessory proteins (3a, 3b, 4b, 4c, 5a, 5b and 6b). Overall, strain B1648 is distinct from all the strains reported so far in Europe and other parts of the world.},
  author       = {Avalakuppa Papi Reddy, Vishwanatha Reddy and Theuns, Sebastiaan and Roukaerts, Inge and Zeller, Mark and Matthijnssens, Jelle and Nauwynck, Hans},
  issn         = {1999-4915},
  journal      = {VIRUSES-BASEL},
  keywords     = {B1648,IBV,nephropathogenic,chicken,nonstructural protein,MOUSE HEPATITIS-VIRUS,CORONAVIRUS MERS-COV,TURKEY CORONAVIRUS,CHICKENS,PATHOGENICITY,PROTECTION,SEQUENCE,IDENTIFICATION,EMERGENCE,GENOTYPES},
  language     = {eng},
  number       = {8},
  pages        = {4488--4506},
  title        = {Genetic characterization of the Belgian nephropathogenic infectious bronchitis virus (NIBV) reference strain B1648},
  url          = {http://dx.doi.org/10.3390/v7082827},
  volume       = {7},
  year         = {2015},
}

Altmetric
View in Altmetric
Web of Science
Times cited: