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Osmotic stress modulates the balance between exocytosis and clathrin-mediated endocytosis in Arabidopsis thaliana

Marta Zwiewka, Tomasz Nodzyński, Stephanie Robert UGent, Steffen Vanneste UGent and Jiri Friml (2015) MOLECULAR PLANT. 8(8). p.1175-1187
abstract
The sessile life style of plants creates the need to deal with an often adverse environment, in which water availability can change on a daily basis, challenging the cellular physiology and integrity. Changes in osmotic conditions disrupt the equilibrium of the plasma membrane: hypoosmotic conditions increase and hyperosmotic environment decrease the cell volume. Here, we show that short-term extracellular osmotic treatments are closely followed by a shift in the balance between endocytosis and exocytosis in root meristem cells. Acute hyperosmotic treatments (ionic and nonionic) enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis, whereas hypoosmotic treatments have the opposite effects. In addition to clathrin recruitment to the plasma membrane, components of early endocytic trafficking are essential during hyperosmotic stress responses. Consequently, growth of seedlings defective in elements of clathrin or early endocytic machinery is more sensitive to hyperosmotic treatments. We also found that the endocytotic response to a change of osmotic status in the environment is dominant over the presumably evolutionary more recent regulatory effect of plant hormones, such as auxin. These results imply that osmotic perturbation influences the balance between endocytosis and exocytosis acting through clathrin-mediated endocytosis. We propose that tension on the plasma membrane determines the addition or removal of membranes at the cell surface, thus preserving cell integrity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SALT-STRESS, DEPENDENT ENDOCYTOSIS, PLANT DEVELOPMENT, PLASMA-MEMBRANE, TOBACCO POLLEN TUBES, SURFACE-AREA REGULATION, AUXIN-BINDING PROTEIN-1, auxin, clathrin-mediated endocytosis, protein trafficking, osmotic stress, DUNALIELLA-SALINA, ORGAN DEVELOPMENT, ION HOMEOSTASIS
journal title
MOLECULAR PLANT
Mol. Plant.
volume
8
issue
8
pages
1175 - 1187
Web of Science type
Article
Web of Science id
000359136100006
JCR category
PLANT SCIENCES
JCR impact factor
7.142 (2015)
JCR rank
6/209 (2015)
JCR quartile
1 (2015)
ISSN
1674-2052
DOI
10.1016/j.molp.2015.03.007
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
6929996
handle
http://hdl.handle.net/1854/LU-6929996
date created
2015-09-14 16:18:30
date last changed
2016-12-19 15:40:00
@article{6929996,
  abstract     = {The sessile life style of plants creates the need to deal with an often adverse environment, in which water availability can change on a daily basis, challenging the cellular physiology and integrity. Changes in osmotic conditions disrupt the equilibrium of the plasma membrane: hypoosmotic conditions increase and hyperosmotic environment decrease the cell volume. Here, we show that short-term extracellular osmotic treatments are closely followed by a shift in the balance between endocytosis and exocytosis in root meristem cells. Acute hyperosmotic treatments (ionic and nonionic) enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis, whereas hypoosmotic treatments have the opposite effects. In addition to clathrin recruitment to the plasma membrane, components of early endocytic trafficking are essential during hyperosmotic stress responses. Consequently, growth of seedlings defective in elements of clathrin or early endocytic machinery is more sensitive to hyperosmotic treatments. We also found that the endocytotic response to a change of osmotic status in the environment is dominant over the presumably evolutionary more recent regulatory effect of plant hormones, such as auxin. These results imply that osmotic perturbation influences the balance between endocytosis and exocytosis acting through clathrin-mediated endocytosis. We propose that tension on the plasma membrane determines the addition or removal of membranes at the cell surface, thus preserving cell integrity.},
  author       = {Zwiewka, Marta and Nodzy\'{n}ski, Tomasz and Robert, Stephanie and Vanneste, Steffen and Friml, Jiri},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  keyword      = {SALT-STRESS,DEPENDENT ENDOCYTOSIS,PLANT DEVELOPMENT,PLASMA-MEMBRANE,TOBACCO POLLEN TUBES,SURFACE-AREA REGULATION,AUXIN-BINDING PROTEIN-1,auxin,clathrin-mediated endocytosis,protein trafficking,osmotic stress,DUNALIELLA-SALINA,ORGAN DEVELOPMENT,ION HOMEOSTASIS},
  language     = {eng},
  number       = {8},
  pages        = {1175--1187},
  title        = {Osmotic stress modulates the balance between exocytosis and clathrin-mediated endocytosis in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1016/j.molp.2015.03.007},
  volume       = {8},
  year         = {2015},
}

Chicago
Zwiewka, Marta, Tomasz Nodzyński, Stephanie Robert, Steffen Vanneste, and Jiri Friml. 2015. “Osmotic Stress Modulates the Balance Between Exocytosis and Clathrin-mediated Endocytosis in Arabidopsis Thaliana.” Molecular Plant 8 (8): 1175–1187.
APA
Zwiewka, M., Nodzyński, T., Robert, S., Vanneste, S., & Friml, J. (2015). Osmotic stress modulates the balance between exocytosis and clathrin-mediated endocytosis in Arabidopsis thaliana. MOLECULAR PLANT, 8(8), 1175–1187.
Vancouver
1.
Zwiewka M, Nodzyński T, Robert S, Vanneste S, Friml J. Osmotic stress modulates the balance between exocytosis and clathrin-mediated endocytosis in Arabidopsis thaliana. MOLECULAR PLANT. 2015;8(8):1175–87.
MLA
Zwiewka, Marta, Tomasz Nodzyński, Stephanie Robert, et al. “Osmotic Stress Modulates the Balance Between Exocytosis and Clathrin-mediated Endocytosis in Arabidopsis Thaliana.” MOLECULAR PLANT 8.8 (2015): 1175–1187. Print.