Ghent University Academic Bibliography

Advanced

Selection for improved energy use efficiency and drought tolerance in canola results in distinct transcriptome and epigenome changes

Aurine Verkest, Marina Byzova, Cindy Martens UGent, Patrick Willems UGent, Tom Verwulgen, Bram Slabbinck, Debbie Rombaut UGent, Jan Van de Velde, Klaas Vandepoele UGent, Evi Standaert, et al. (2015) PLANT PHYSIOLOGY. 168(4). p.1338-1350
abstract
To increase both the yield potential and stability of crops, integrated breeding strategies are used that have mostly a direct genetic basis, but the utility of epigenetics to improve complex traits is unclear. A better understanding of the status of the epigenome and its contribution to agronomic performance would help in developing approaches to incorporate the epigenetic component of complex traits into breeding programs. Starting from isogenic canola (Brassica napus) lines, epilines were generated by selecting, repeatedly for three generations, for increased energy use efficiency and drought tolerance. These epilines had an enhanced energy use efficiency, drought tolerance, and nitrogen use efficiency. Transcriptome analysis of the epilines and a line selected for its energy use efficiency solely revealed common differentially expressed genes related to the onset of stress tolerance-regulating signaling events. Genes related to responses to salt, osmotic, abscisic acid, and drought treatments were specifically differentially expressed in the drought-tolerant epilines. The status of the epigenome, scored as differential trimethylation of lysine-4 of histone 3, further supported the phenotype by targeting drought-responsive genes and facilitating the transcription of the differentially expressed genes. From these results, we conclude that the canola epigenome can be shaped by selection to increase energy use efficiency and stress tolerance. Hence, these findings warrant the further development of strategies to incorporate epigenetics into breeding.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
H3 LYSINE 4, MODEL DATA-ANALYSIS, ARABIDOPSIS-THALIANA, HISTONE H3, GENE-EXPRESSION, ABSCISIC-ACID, EPIGENETIC MUTATION, OSMOTIC-STRESS, PROBE LEVEL DATA, SEQUENCE-ANALYSIS
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
168
issue
4
pages
1338 - 1350
Web of Science type
Article
Web of Science id
000359317400015
JCR category
PLANT SCIENCES
JCR impact factor
6.28 (2015)
JCR rank
8/209 (2015)
JCR quartile
1 (2015)
ISSN
0032-0889
DOI
10.1104/pp.15.00155
project
Biotechnology for a sustainable economy (Bio-Economy)
project
Bioinformatics: from nucleotids to networks (N2N)
language
English
UGent publication?
yes
classification
A1
additional info
the last two authors contributed equally to this work
copyright statement
I have retained and own the full copyright for this publication
id
6929884
handle
http://hdl.handle.net/1854/LU-6929884
date created
2015-09-14 15:46:25
date last changed
2017-02-22 14:41:21
@article{6929884,
  abstract     = {To increase both the yield potential and stability of crops, integrated breeding strategies are used that have mostly a direct genetic basis, but the utility of epigenetics to improve complex traits is unclear. A better understanding of the status of the epigenome and its contribution to agronomic performance would help in developing approaches to incorporate the epigenetic component of complex traits into breeding programs. Starting from isogenic canola (Brassica napus) lines, epilines were generated by selecting, repeatedly for three generations, for increased energy use efficiency and drought tolerance. These epilines had an enhanced energy use efficiency, drought tolerance, and nitrogen use efficiency. Transcriptome analysis of the epilines and a line selected for its energy use efficiency solely revealed common differentially expressed genes related to the onset of stress tolerance-regulating signaling events. Genes related to responses to salt, osmotic, abscisic acid, and drought treatments were specifically differentially expressed in the drought-tolerant epilines. The status of the epigenome, scored as differential trimethylation of lysine-4 of histone 3, further supported the phenotype by targeting drought-responsive genes and facilitating the transcription of the differentially expressed genes. From these results, we conclude that the canola epigenome can be shaped by selection to increase energy use efficiency and stress tolerance. Hence, these findings warrant the further development of strategies to incorporate epigenetics into breeding.},
  author       = {Verkest, Aurine and Byzova, Marina and Martens, Cindy and Willems, Patrick and Verwulgen, Tom and Slabbinck, Bram and Rombaut, Debbie and Van de Velde, Jan and Vandepoele, Klaas and Standaert, Evi and Peeters, Marrit and Van Lijsebettens, Maria and Van Breusegem, Frank and De Block, Marc},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {H3 LYSINE 4,MODEL DATA-ANALYSIS,ARABIDOPSIS-THALIANA,HISTONE H3,GENE-EXPRESSION,ABSCISIC-ACID,EPIGENETIC MUTATION,OSMOTIC-STRESS,PROBE LEVEL DATA,SEQUENCE-ANALYSIS},
  language     = {eng},
  number       = {4},
  pages        = {1338--1350},
  title        = {Selection for improved energy use efficiency and drought tolerance in canola results in distinct transcriptome and epigenome changes},
  url          = {http://dx.doi.org/10.1104/pp.15.00155},
  volume       = {168},
  year         = {2015},
}

Chicago
Verkest, Aurine, Marina Byzova, Cindy Martens, Patrick Willems, Tom Verwulgen, Bram Slabbinck, Debbie Rombaut, et al. 2015. “Selection for Improved Energy Use Efficiency and Drought Tolerance in Canola Results in Distinct Transcriptome and Epigenome Changes.” Plant Physiology 168 (4): 1338–1350.
APA
Verkest, A., Byzova, M., Martens, C., Willems, P., Verwulgen, T., Slabbinck, B., Rombaut, D., et al. (2015). Selection for improved energy use efficiency and drought tolerance in canola results in distinct transcriptome and epigenome changes. PLANT PHYSIOLOGY, 168(4), 1338–1350.
Vancouver
1.
Verkest A, Byzova M, Martens C, Willems P, Verwulgen T, Slabbinck B, et al. Selection for improved energy use efficiency and drought tolerance in canola results in distinct transcriptome and epigenome changes. PLANT PHYSIOLOGY. 2015;168(4):1338–50.
MLA
Verkest, Aurine, Marina Byzova, Cindy Martens, et al. “Selection for Improved Energy Use Efficiency and Drought Tolerance in Canola Results in Distinct Transcriptome and Epigenome Changes.” PLANT PHYSIOLOGY 168.4 (2015): 1338–1350. Print.