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Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles

(2015) JOURNAL OF EXPERIMENTAL BOTANY. 66(16). p.5055-5065
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Abstract
The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identified based on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxinbinding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations. Their analysis revealed that an intact auxin-binding pocket is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association with membranes for endocytosis regulation. In planta analyses demonstrated the importance of the auxin binding pocket for all known ABP1-mediated postembryonic developmental processes, including morphology of leaf epidermal cells, root growth and root meristem activity, and vascular tissue differentiation. Taken together, these findings suggest that auxin binding to ABP1 is central to its function, supporting the role of ABP1 as auxin receptor.
Keywords
TRAFFICKING, PROTOPLASTS, COMPLEX, RECEPTOR, EXPANSION, ENDOCYTOSIS, EFFLUX, Site-directed mutagenesis, PIN proteins, BOX PROTEIN TIR1, PLASMA-MEMBRANE, ARABIDOPSIS, Auxin binding, ABP1, Auxin

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Citation

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Chicago
Grones, Peter, Xu Chen, Sibu Simon, Walter A Kaufmann, Riet De Rycke, Tomasz Nodzyński, Eva Zažímalová, and Jiri Friml. 2015. “Auxin-binding Pocket of ABP1 Is Crucial for Its Gain-of-function Cellular and Developmental Roles.” Journal of Experimental Botany 66 (16): 5055–5065.
APA
Grones, P., Chen, X., Simon, S., Kaufmann, W. A., De Rycke, R., Nodzyński, T., Zažímalová, E., et al. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. JOURNAL OF EXPERIMENTAL BOTANY, 66(16), 5055–5065.
Vancouver
1.
Grones P, Chen X, Simon S, Kaufmann WA, De Rycke R, Nodzyński T, et al. Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. JOURNAL OF EXPERIMENTAL BOTANY. 2015;66(16):5055–65.
MLA
Grones, Peter, Xu Chen, Sibu Simon, et al. “Auxin-binding Pocket of ABP1 Is Crucial for Its Gain-of-function Cellular and Developmental Roles.” JOURNAL OF EXPERIMENTAL BOTANY 66.16 (2015): 5055–5065. Print.
@article{6929642,
  abstract     = {The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identified based on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxinbinding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations. Their analysis revealed that an intact auxin-binding pocket is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association with membranes for endocytosis regulation. In planta analyses demonstrated the importance of the auxin binding pocket for all known ABP1-mediated postembryonic developmental processes, including morphology of leaf epidermal cells, root growth and root meristem activity, and vascular tissue differentiation. Taken together, these findings suggest that auxin binding to ABP1 is central to its function, supporting the role of ABP1 as auxin receptor.},
  author       = {Grones, Peter and Chen, Xu and Simon, Sibu and Kaufmann, Walter A and De Rycke, Riet and Nodzy\'{n}ski, Tomasz and Za\v{z}{\'i}malov{\'a}, Eva and Friml, Jiri},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keyword      = {TRAFFICKING,PROTOPLASTS,COMPLEX,RECEPTOR,EXPANSION,ENDOCYTOSIS,EFFLUX,Site-directed mutagenesis,PIN proteins,BOX PROTEIN TIR1,PLASMA-MEMBRANE,ARABIDOPSIS,Auxin binding,ABP1,Auxin},
  language     = {eng},
  number       = {16},
  pages        = {5055--5065},
  title        = {Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles},
  url          = {http://dx.doi.org/10.1093/jxb/erv177},
  volume       = {66},
  year         = {2015},
}

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