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The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation

(2015) JOURNAL OF EXPERIMENTAL BOTANY. 66(17). p.5245-5256
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Abstract
Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses. In addition, our previous report suggested that they also participate in the formation of lateral roots. Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots. To investigate the cause of this phenotype we analysed primordium development in gain-of-function (gof) mutants and found that GLV6 induces supernumerary pericycle divisions, hindering the formation of a dome-shaped primordium, a prerequisite for successful emergence. The GLV6 phenotype could be reproduced by ectopic expression of the gene only in xylem-pole pericycle cells. Furthermore, GLV6 seems to function at the very beginning of lateral root initiation because GLV6 excess-either gene overexpression or peptide treatment-disrupts the first asymmetric cell divisions required for proper primordium formation. Our results suggest that GLV6 acts during lateral root initiation controlling the patterning of the first pericycle divisions.
Keywords
SECRETED PEPTIDE, AUXIN TRANSPORT, PLANT DEVELOPMENT, ARABIDOPSIS-THALIANA, SMALL SIGNALING PEPTIDES, secreted peptides, root growth factors, primordium initiation, lateral root development, GOLVEN, CLE-like, Arabidopsis thaliana, Asymmetric division, FOUNDER CELLS, EXPRESSION, MOLECULES, MARKER, GROWTH

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Chicago
Fernandez Salina, Ana, Andrzej Drozdzecki, Kurt Hoogewijs, Valya Vassileva, Annemieke Madder, Tom Beeckman, and Pierre Hilson. 2015. “The GLV6/RGF8/CLEL2 Peptide Regulates Early Pericycle Divisions During Lateral Root Initiation.” Journal of Experimental Botany 66 (17): 5245–5256.
APA
Fernandez Salina, A., Drozdzecki, A., Hoogewijs, K., Vassileva, V., Madder, A., Beeckman, T., & Hilson, P. (2015). The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation. JOURNAL OF EXPERIMENTAL BOTANY, 66(17), 5245–5256.
Vancouver
1.
Fernandez Salina A, Drozdzecki A, Hoogewijs K, Vassileva V, Madder A, Beeckman T, et al. The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation. JOURNAL OF EXPERIMENTAL BOTANY. 2015;66(17):5245–56.
MLA
Fernandez Salina, Ana et al. “The GLV6/RGF8/CLEL2 Peptide Regulates Early Pericycle Divisions During Lateral Root Initiation.” JOURNAL OF EXPERIMENTAL BOTANY 66.17 (2015): 5245–5256. Print.
@article{6929583,
  abstract     = {Small peptides of the Arabidopsis GLV/RGF/CLEL family are involved in different developmental programmes, including meristem maintenance and gravitropic responses. In addition, our previous report suggested that they also participate in the formation of lateral roots. Specifically, GLV6 is transcribed during the first stages of primordium development and GLV6 overexpression results in a strong reduction of emerged lateral roots. To investigate the cause of this phenotype we analysed primordium development in gain-of-function (gof) mutants and found that GLV6 induces supernumerary pericycle divisions, hindering the formation of a dome-shaped primordium, a prerequisite for successful emergence. The GLV6 phenotype could be reproduced by ectopic expression of the gene only in xylem-pole pericycle cells. Furthermore, GLV6 seems to function at the very beginning of lateral root initiation because GLV6 excess-either gene overexpression or peptide treatment-disrupts the first asymmetric cell divisions required for proper primordium formation. Our results suggest that GLV6 acts during lateral root initiation controlling the patterning of the first pericycle divisions.},
  author       = {Fernandez Salina, Ana and Drozdzecki, Andrzej and Hoogewijs, Kurt and Vassileva, Valya and Madder, Annemieke and Beeckman, Tom and Hilson, Pierre},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keywords     = {SECRETED PEPTIDE,AUXIN TRANSPORT,PLANT DEVELOPMENT,ARABIDOPSIS-THALIANA,SMALL SIGNALING PEPTIDES,secreted peptides,root growth factors,primordium initiation,lateral root development,GOLVEN,CLE-like,Arabidopsis thaliana,Asymmetric division,FOUNDER CELLS,EXPRESSION,MOLECULES,MARKER,GROWTH},
  language     = {eng},
  number       = {17},
  pages        = {5245--5256},
  title        = {The GLV6/RGF8/CLEL2 peptide regulates early pericycle divisions during lateral root initiation},
  url          = {http://dx.doi.org/10.1093/jxb/erv329},
  volume       = {66},
  year         = {2015},
}

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