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Antagonistic peptide technology for functional dissection of CLE peptides revisited

(2015) JOURNAL OF EXPERIMENTAL BOTANY. 66(17). p.5367-5374
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Abstract
In the Arabidopsis thaliana genome, over 1000 putative genes encoding small, presumably secreted, signalling peptides can be recognized. However, a major obstacle in identifying the function of genes encoding small signalling peptides is the limited number of available loss-of-function mutants. To overcome this, a promising new tool, antagonistic peptide technology, was recently developed. Here, this antagonistic peptide technology was tested on selected CLE peptides and the related IDA peptide and its usefulness in the context of studies of peptide function discussed. Based on the analyses, it was concluded that the antagonistic peptide approach is not the ultimate means to overcome redundancy or lack of loss-of-function lines. However, information collected using antagonistic peptide approaches (in the broad sense) can be very useful, but these approaches do not work in all cases and require a deep insight on the interaction between the ligand and its receptor to be successful. This, as well as peptide ligand structure considerations, should be taken into account before ordering a wide range of synthetic peptide variants and/or generating transgenic plants.
Keywords
NODULATION, CLV3, ROOT-MERISTEM, GENE-EXPRESSION, ARABIDOPSIS-THALIANA, PROTOPHLOEM DIFFERENTIATION, INFLORESCENCE-DEFICIENT, RECEPTOR-LIKE KINASES, STEM-CELL FATE, small signalling peptides, root, peptide variants, peptide structure, IDA, CLE, ABSCISSION

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Chicago
Czyzewicz, Nathan, Mari Wildhagen, Pietro Cattaneo, Yvonne Stahl, Karine Gustavo Pinto, Reidunn B Aalen, Melinka A Butenko, Rüdiger Simon, Christian S Hardtke, and Ive De Smet. 2015. “Antagonistic Peptide Technology for Functional Dissection of CLE Peptides Revisited.” Journal of Experimental Botany 66 (17): 5367–5374.
APA
Czyzewicz, N., Wildhagen, M., Cattaneo, P., Stahl, Y., Pinto, K. G., Aalen, R. B., Butenko, M. A., et al. (2015). Antagonistic peptide technology for functional dissection of CLE peptides revisited. JOURNAL OF EXPERIMENTAL BOTANY, 66(17), 5367–5374.
Vancouver
1.
Czyzewicz N, Wildhagen M, Cattaneo P, Stahl Y, Pinto KG, Aalen RB, et al. Antagonistic peptide technology for functional dissection of CLE peptides revisited. JOURNAL OF EXPERIMENTAL BOTANY. 2015;66(17):5367–74.
MLA
Czyzewicz, Nathan, Mari Wildhagen, Pietro Cattaneo, et al. “Antagonistic Peptide Technology for Functional Dissection of CLE Peptides Revisited.” JOURNAL OF EXPERIMENTAL BOTANY 66.17 (2015): 5367–5374. Print.
@article{6929465,
  abstract     = {In the Arabidopsis thaliana genome, over 1000 putative genes encoding small, presumably secreted, signalling peptides can be recognized. However, a major obstacle in identifying the function of genes encoding small signalling peptides is the limited number of available loss-of-function mutants. To overcome this, a promising new tool, antagonistic peptide technology, was recently developed. Here, this antagonistic peptide technology was tested on selected CLE peptides and the related IDA peptide and its usefulness in the context of studies of peptide function discussed. Based on the analyses, it was concluded that the antagonistic peptide approach is not the ultimate means to overcome redundancy or lack of loss-of-function lines. However, information collected using antagonistic peptide approaches (in the broad sense) can be very useful, but these approaches do not work in all cases and require a deep insight on the interaction between the ligand and its receptor to be successful. This, as well as peptide ligand structure considerations, should be taken into account before ordering a wide range of synthetic peptide variants and/or generating transgenic plants.},
  author       = {Czyzewicz, Nathan and Wildhagen, Mari and Cattaneo, Pietro and Stahl, Yvonne and Pinto, Karine Gustavo and Aalen, Reidunn B and Butenko, Melinka A and Simon, R{\"u}diger and Hardtke, Christian S and De Smet, Ive},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  language     = {eng},
  number       = {17},
  pages        = {5367--5374},
  title        = {Antagonistic peptide technology for functional dissection of CLE peptides revisited},
  url          = {http://dx.doi.org/10.1093/jxb/erv284},
  volume       = {66},
  year         = {2015},
}

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